1981
DOI: 10.1016/0003-9861(81)90121-1
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The relationship of the quaternary structure of allophycocyanin to its spectrum

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Cited by 54 publications
(29 citation statements)
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“…The fluorescence maximum of the 6s fraction of APC I in its form (a,8)3y', where y' is the 35 kD degradation product of the 95 kD polypeptide, remains at 675 nm with decreased ionic strength; this is not surprising, as there was no indication ofionic strength dependence ofthe 675 nm fluorescence of this component in former studies (20). These (13,18) and with monomeric to trimeric association of APC itself (9).…”
Section: Methodsmentioning
confidence: 81%
“…The fluorescence maximum of the 6s fraction of APC I in its form (a,8)3y', where y' is the 35 kD degradation product of the 95 kD polypeptide, remains at 675 nm with decreased ionic strength; this is not surprising, as there was no indication ofionic strength dependence ofthe 675 nm fluorescence of this component in former studies (20). These (13,18) and with monomeric to trimeric association of APC itself (9).…”
Section: Methodsmentioning
confidence: 81%
“…2B, lane 2, was evaluated, it was found that 100% of ApcB was chromophorylated, while only ϳ40% of HT-ApcA was chromophorylated. At sufficiently high protein concentrations and under appropriate ionic strength and pH conditions, AP forms (␣␤) 3 trimers that result in a characteristic red shift in the absorbance ( 653 ) and fluorescence ( 663 ) properties of the protein (6,30,31,39). When the linker protein ApcC was present, it also sharpens the absorbance spectrum of trimers (47).…”
Section: Resultsmentioning
confidence: 99%
“…The concentration of the complex was adjusted to 6*10 Ϫ8 M, far above the dissociation constant of approx. 5*10 Ϫ10 M [24,29]. The fluorescence was excited at a wavelength of 647 nm and the emission detected in the range above 665 nm, identical to the parameter setting in the CLSM measurements.…”
Section: ϫ5mentioning
confidence: 99%