The relationships between microbiome diversity and epidemiology in domestic species of malaria-mediated mosquitoes of Korea

Abstract

Microbiota in the mosquito plays an important role in their behavior and vector competence. The composition of their microbiome is strongly influenced by the environment, especially their habitat. The microbiome profiles of adult female Anopheles sinensis mosquitoes from malaria hyperendemic and hypoendemic areas in Republic of Korea were compared using 16S rRNA Illumina sequencing. In different epidemiology groups, the alpha and beta diversity analyses were significant. The major bacterial phylum was Proteobacteria. The most abundant species in the microbiome of hyperendemic mosquitoes were the genera Staphylococcus, Erwinia, Serratia, and Pantoea. Notably, a distinct microbiome profile characterized by the dominance of Pseudomonas synxantha was identified in the hypoendemic area, suggesting a potential correlation between the microbiome profiles and the incidence of malaria cases.

Geographical Characteristics of Culex tritaeniorhynchus and Culex orientalis Microbiomes in Korea

“…The mosquito microbiome comprises a core microbiome essential for basic physiological functions and an environmental microbiome specific to particular habitats [ 35 ]. These characteristics of the mosquito microbiome were utilized to identify species biomarkers by considering the regional specificity and abundance of microorganisms [ 23 ]. These species biomarkers will be used as crucial biological clues for tracing mosquito movement.…”

“…Microbial community composition is influenced by the host’s genetic background, prolonged interactions with bacteria, and relationships between the microbiome and infectious pathogens. Previous studies have demonstrated distinct profiles in mosquito microbiomes in areas with and without a high prevalence of malaria [ 23 ]. Given the past detection of JEV in Cx.…”

“…DNA sequence data were analyzed using QIIME2, as described previously [ 23 , 25 ]. The reads were denoised and trimmed using the DADA2 pipeline [ 26 ].…”

“…Taxonomic biomarkers were searched as previously reported [ 23 ]. The abundance of data from each mosquito species was analyzed for the presence/absence of microbes.…”

Gut Microbiota of Apis mellifera at Selected Ontogenetic Stages and Their Immunogenic Potential during Summer

“…The Kruskal–Wallis H test was used to compare the results among developmental stages. To discover significant differences in their bacterial profiles, pairwise PERMANOVA was performed with the “beta-group-significance” tool in QIIME2 [ 44 ].…”