The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in<i>c-kit</i>Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells

Sailasuta, Achariya; Ketpun, Dettachai; Piyaviriyakul, Prapruddee; Theerawatanasirikul, Sirin; Theewasutrakul, Patrakrit; Rungsipipat, Anudep

doi:10.1155/2014/787498

Cited by 23 publications

(20 citation statements)

References 19 publications

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“…Some intermediate-grade tumors have a benign behavior, while others exhibit aggressive growth and a high frequency of local and distant recurrence [23]. CD117 (KIT) is a tyrosine kinase receptor protein expressed on the surface of bone marrow precursors (myeloid, erythroid, mouse but not human pro B-blast and pro T-blast cells) and mature mast cells [25][26][27], and it is a useful marker in the diagnosis of mast cell disorders and gastrointestinal stromal tumors [26].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Diagnostic procedures for improving of the KIT (CD117) expressed allele burden for the liver metastases from uterus mast cell tumors: prognostic value of the metastatic pattern and tumor biology

et al. 2014

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The activating KIT marker plays a central role in the pathogenesis, diagnosis, and targeted treatment of systemic mastocytosis (SM). Recent studies have identified the KIT (CD117) as a marker that distinguishes nonneoplastic from neoplastic mast cells in human systemic mastocytosis. In this study, we conclude that immunohistopathology assays for KIT staining pattern are useful complimentary tools for diagnosis and evaluation of prognosis in uterus mast cell tumor (MCT) metastasis to the liver in 10 patients. Uterine and hepatic cytology revealed mast cell neoplasia, which was confirmed as visceral mast cell tumor on postmortem examination. Histological changes of densely packed, poorly differentiated neoplastic mast cells, sheets of neoplastic round to pleomorphic cells that formed nonencapsulated nodules, high mitotic figures, necrosis, and fibrosis were found. In addition, eosinophils were scattered among the mast cells at the periphery of the nodules. These findings indicate tumors of high-grade malignancy with infiltrative cells resembling the uterus MCT in the intraparenchymal and periparenchymal areas of the liver. Immunohistochemically, tumors were positive for KIT. The histopathologic features coupled with the KIT immunoreactivity led to diagnosis of high-grade uterus MCTs. Taken together, these findings suggest that CD117 may play a critical role in early uterus MCT development and may be a stimulatory factor in grade 3 MCT. Therefore, the result has supported our hypothesis that there was an increased opportunity to observe a higher CD117 staining pattern in high-grade MCTs.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Diagnostic procedures for improving of the KIT (CD117) expressed allele burden for the liver metastases from uterus mast cell tumors: prognostic value of the metastatic pattern and tumor biology

et al. 2014

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“…The impact of genetic factors cannot be excluded either. The presence of a c-kit gene mutation in the nuclear genome in mast cell tumours with a higher malignancy grade has been reported (Zemke et al 2002, Sailasuta et al 2014. Moreover, as demonstrated by Zemke et al 2002, there was no relationship between the presence of the mutation and the Epidemiological study of canine mast cell tumours...dog's breed.…”

Section: Discussionmentioning

confidence: 97%

Epidemiological Study of Canine Mast Cell Tumours According to the Histological Malignancy Grade

Śmiech¹,

Ślaska²,

Łopuszyński³

et al. 2017

Polish Journal of Veterinary Sciences

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The aim of the study was to identify significant relationships between the tumour malignancy grade and dogs' age, breed, sex, size, and location of mast cell tumours (MCTs). MCTs accounted for 13.27% of all diagnosed canine skin tumours. The highest incidence was recorded among Boxers, Labrador Retrievers, American Staffordshire Terriers, and Golden Retrievers. Statistical analysis revealed significantly higher probability of occurrence of the grade I mast cell tumour in the French Bulldog in the head, neck, torso, and limb regions, the grade-II mast cell tumour in Boxer, Doberman, Dachshund, shepherds, and setters in the scrotal region, and the grade III mast cell tumour in Shar-Pei in the axilla region. In the group of the oldest dogs aged 11-16, there was higher risk of development of MCTs grade II and III. Young dogs (aged 2-3 and 4-6) were found to be more prone to development of MCTs grade I. There was no correlation between MCTs grade and dogs' sex and size. To the authors' knowledge this is the first report on statistical relationships between the degree of mast cell tumour malignancy and dogs' phenotypic traits, age and tumour location. This analysis indicate predilections for development of the particular mast cell tumour malignancy degrees in certain dog breeds, age, and anatomical location.

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“…C-kit mutations are mostly the characteristic of CMCTs of higher histological grade [9,15,23,24]. It has been suggested that these mutations play a role in the malignant transformation of CMCTs [24].…”

Section: Discussionmentioning

confidence: 99%

Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation

et al. 2016

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Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classifi cation. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for fi nal histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfi xed and paraffi n-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffi n blocks and subsequent polymerase chain reaction amplifi cation was performed using PE1 and PE2 primers. Degree of malignancy was determined based on the presence of mitotic fi gures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fi elds. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classifi ed as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confi rms the link between the aberrant CD117 expression and increased cell proliferation.

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The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 inc-kitDetected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells

Cited by 23 publications

References 19 publications

RETRACTED ARTICLE: Diagnostic procedures for improving of the KIT (CD117) expressed allele burden for the liver metastases from uterus mast cell tumors: prognostic value of the metastatic pattern and tumor biology

RETRACTED ARTICLE: Diagnostic procedures for improving of the KIT (CD117) expressed allele burden for the liver metastases from uterus mast cell tumors: prognostic value of the metastatic pattern and tumor biology

Epidemiological Study of Canine Mast Cell Tumours According to the Histological Malignancy Grade

Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation

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