The Relevance of Hypothalamic and Hyphophyseal Progestin Receptor Regulation in the Induction and Inhibition of Sexual Behavior in the Female Rat

Moguilewsky, M.; Raynaud, Jean‐Pierre

doi:10.1210/endo-105-2-516

Cited by 165 publications

(49 citation statements)

References 42 publications

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“…One notable exception was the autoradiographic study by Sar and Stumpf (1973) guinea pig. However, the recent availability of a high affinity synthetic progestin, r3H]R5020, has facilitated the identification of specific progestin receptors in the brain and pituitary in ovariectomized and estrogen-stimulated rats (Moguilewsky and Raynaud, 1979;Kato and Onouchi, 1977;MacLusky and McEwen, 1978,198O;Warembourg, 1978), guinea pigs Feder, 1979, 1980), and the bonnet monkey . One major feature of central and pituitary progestin receptor systems is the presence of a class of receptors which is unaffected by estrogenic stimulation as well as a class of receptors which is induced by EZ (MacLusky andMcEwen, 1978, 1980).…”

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confidence: 99%

“…One major feature of central and pituitary progestin receptor systems is the presence of a class of receptors which is unaffected by estrogenic stimulation as well as a class of receptors which is induced by EZ (MacLusky andMcEwen, 1978, 1980). Although the physiological significance of these two classes of progestin receptors is unclear, estrogen-inducible progestin receptors appear to be concentrated in those areas of the brain implicated in the control of sexual receptivity and gonadotropin secretion, the mediobasal hypothalamus (MBH) and preoptic of Neuroscience Progestin Receptors in Rat Brain 10 f 1 6-9 43 + 2 6-8 10 ?I 1 10-14 48 k 4 10-14 13 f 1 area (POA) (Moguilewsky and Raynaud, 1979;Kato and Onouchi, 1977;McEwen, 1978, 1980;Warembourg, 1978;Blaustein and Feder, 1979). Moreover, the induction and decay of estrogen-inducible progestin receptors in the MBH-POA of the female rat (Parsons et al, 1980) and guinea pig (Blaustein and Feder, 1979) have been shown to be correlated temporally with the induction and decay of progesterone-facilitated sexual receptivity.…”

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confidence: 99%

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Progestin receptor levels in rat hypothalamic and limbic nuclei

Parsons¹,

Rainbow²,

Nj³

et al. 1982

J. Neurosci.

257

108

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We have utilized a method to minimize cytosol progestin receptor loss during freezing in order to localize and quantify estrogen-inducible progestin receptors in individual nuclei of the female rat brain.Ovariectomized females received estradiol benzoate (20 pg for 3 days) or vehicle prior to sacrifice. All animals were perfused with cold distilled Hz0 containing the cryoprotective compound, dimethyl sulfoxide (DMSO; 10% (v/v)). Thirty-one nuclei or brain regions were removed from frozen sections (300 pm) according to the method of Palkovits (Palkovits, M. (1973) Brain Res. 59: 449-450) and were assayed in vitro using a synthetic radioligand, r3H]R5020.In ovariectomized animals perfused with DMSO, a basal level (1 to 8 fmol/mg of protein) of progestin receptors was observed in a variety of preoptic, hypothalamic, and limbic structures. Moreover, estrogen treatment induced high levels (24 to 49 fmol/mg of protein) of progestin receptors in regions of the preoptic area of hypothalamus which contain high levels of estrogen receptors. These regions included the medial, periventricular, and superchiasmatic nuclei of the preoptic area, the periventricular anterior hypothalamus, the ventromedial nucleus, and the arcuatemedian eminence. Moderate levels (2 to 8 fmol/mg of protein) of progestin receptors were induced by estrogen in other hypothalamic and limbic structures, including the anterior and lateral hypothalamus, the bed nucleus of the stria terminalis, the cingulate cortex, the medial amygdaloid nucleus, and the CA, subfield of the hippocampus. By contrast, some areas, such .as the caudateputamen and the supraoptic nucleus, were devoid of both estrogen-inducible and uninduced progestin receptors.These results support the hypothesis that progesterone action in the central nervous system is mediated by cytosol receptors in discrete brain regions and provide the first quantitative map of progestin binding in a vertebrate brain.Progesterone (P) and estradiol (E2) synergize to activate feminine reproductive behavior and to induce gonadotropin secretion in a number of vertebrate species, including the rat and guinea pig. Previous studies which have employed [3H]progesterone as a radioligand usually have failed to identify specific progestin-binding components which could mediate the central actions of P in the vertebrate brain (for references, see Feder and Marrone, 1978;McEwen, 1978).

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confidence: 99%

mentioning

confidence: 99%

Progestin receptor levels in rat hypothalamic and limbic nuclei

Parsons¹,

Rainbow²,

Nj³

et al. 1982

J. Neurosci.

257

108

View full text Add to dashboard Cite

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“…Another purpose of these experiments was to investigate the timecourse of the appearance of feminine sexual behavior in animals of both sexes treated under the same experimental conditions as Discussion Previous studies had reported little or no sex difference in the level of CPR in whole MBH (21)(22)(23)(24)(25)28). However, when individual brain cell groupings were examined with microdissection techniques, higher levels of progestin receptor binding were observed in VMN cytosol of female rats (26,27).…”

Section: T Treatment Fleet On Expression Offeminine Sexual Behaviormentioning

confidence: 98%

“…Display of lordosis behavior after E, treatment as well as induction of P receptors in MBH nuclei have been correlated with each other (12,13,15,17,21,24,25,29), with the VMN being one of the most important nuclei regulating the expression of this behavior (2, 10, 31). When we compared CPR levels for each MBH nucleus with the expression of feminine sexual behavior at different times of E, exposure, only the VMN showed a high correlation between CPR density and lordosis behavior ( Table 2).…”

Section: E-induced P Receptor Levels In Diferent Brain Nucleimentioning

confidence: 99%

“…Early studies comparing cytosolic progestin receptor (CPR) levels in the whole hypothalamic-preoptic area of estrogen-primed male and female rats were contradictory, with some reports indicating lower P receptor levels in male rats and others showing little or no sex differences (21)(22)(23)(24)(25). Rainbow et al (26), utilizing cytosol obtained from microdissected brain nuclei, reported sex differences of CPR levels in the ventromedial nuclei (VMN) in EBprimed animals.…”

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Progestin Receptor Induction and Sexual Behavior by Estradiol Treatment in Male and Female Rats

Coirini

McEwen

1990

J Neuroendocrinology

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Sex differences in the induction of cytosolic progestin receptors (CPR) by estrogen priming were correlated with the sex differences in behavioral responses. We evaluated the temporal relationship between CPR in several brain regions and pituitary and the timecourse of 17P-estradiol (E,) activation of female sexual behavior in gonadectomized male and female rats implanted with sribcutaneous E, Silastic capsules for 6 h, 24 h and 48 h. Both CPR levels and mating behavior increase monotonically with the time 0; E, exposure. Induction of CPR was observed in the periventricular region of the preoptic area (PVPOA), arcuate nucleus (ARC), ventromedial nuclei (VMN) and pituitary in both sexes. A small induction of CPR was found in parietal cortex. The VMN in female rats showed a significant E,-induced CPR increase a t all times of exposure, while in male rats this induction was only significant after 24 h. Significant sex differences in absolute CPR levels and E,-induced receptors were found in the following structures: VMN, 18 h after 6 h of E, treatment and after 24 h and 48 h of continuous E, exposure; PVPOA, only after 48 h of continuous E, exposure; ARC at 24 h and 48 h; and pituitary after all E, treatment. Mating behavior was tested under two conditions: E, alone (2 h after removal of E, capsules) and E,+ progesterone (2 h after a progesterone injection given 10 min after concluding the first test). Receptivity was first observed after 24 h E, exposure in female rats, whereas in male rats a small response appeared only after 48 h of E, exposure. After progesterone priming, the time of E, exposure necessary for expression of female sexual behavior was reduced to 6 h in females and 24 h in males. The appearance of mating behavior appears to follow that of inducible CPR in the VMN in both sexes. In addition, the CPR levels associated with the first receptivity either by male rats (16.6 fmol/mg protein) or female rats (15.3 fmol/mg protein) are very similar suggesting the presence of a threshold level controlling the expression of feminine sexual behavior. It is likely that in!iibitory neural input plays a role in determining the threshold level of E,-induced CPR, which is sufficient to trigger lordosis behavior.Thc sequential secretion of ovarian steroid 17B-estradiol (E,) and Progesterone (P) during the estrous cycle, interact synergistically controlling the onset, quality and duration of the female rat sexual behavior (1, 2). The gonadal steroid effect on this behavior expression can be mimicked in ovariectomized female rats by trei tment with physiological doses of estrogens followed by P priming (3, 4).In male rats the expression of female sexual behavior is not normally expressed. However, chronic administration of estradiol kilxoate (EB) in various dosages to castrated males resulted in a moderate increase of the lordosis behavior (5-7) which is poorly or not at all increased by P treatment (8,9). The sex differences in the response to E, and P treatment have been attributed to diff'crences in the est...

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Estrogen/progesterone treatment in adulthood affects the size of several components of the medial preoptic area in the male rat

Bloch¹,

Gorski²

1988

J of Comparative Neurology

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The results of preliminary studies suggested that steroid and/or propylthiouracil (PTU) treatment of adult gonadectomized (Gxd) male rats significantly reduced the volume of the sexually dimorphic nucleus of the preoptic area (SDN-POA). Therefore, we designed a study to examine this effect in detail. Groups of adult rats were sham Gxd (intact) or Gxd, then treated with multiple injections of oil (males and females), or estrogen and progesterone (males). Gonadectomized estrogen/progesterone-treated males had a significantly smaller SDN-POA volume, smaller volume of the medial division of the medial preoptic nucleus (MPNm), smaller volume of the anteroventral MPNm (MPNav), and larger volume of the anteroventral periventricular nucleus (AVPv). The volume of the central division of the medial preoptic nucleus (MPNc) or of the suprachiasmatic nucleus was not affected. There were no differences between Gxd estrogen/progesterone-treated males vs the group that received PTU as well, indicating that the PTU treatment was unnecessary. The reduced volume of the SDN-POA was due to a reduced volume of the MPNav and of the portion of the SDN-POA located within the MPNm-exclusive of the MPNav and MPNc. In conclusion, estrogen/progesterone treatment in adulthood caused significant changes in the volume of several medial preoptic structures in two separate groups of Gxd males. Because the steroids produced no significant effects in intact males, testicular hormones appear to "protect" these structures from the effects of the estrogen/progesterone treatment.

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The Relevance of Hypothalamic and Hyphophyseal Progestin Receptor Regulation in the Induction and Inhibition of Sexual Behavior in the Female Rat

Cited by 165 publications

References 42 publications

Progestin receptor levels in rat hypothalamic and limbic nuclei

Progestin receptor levels in rat hypothalamic and limbic nuclei

Progestin Receptor Induction and Sexual Behavior by Estradiol Treatment in Male and Female Rats

Estrogen/progesterone treatment in adulthood affects the size of several components of the medial preoptic area in the male rat

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