The Renal Humoral Pressor Mechanism in Man. Iv. The Hypertensinogen Content of the Plasma of Normal Patients and Patients With Various Diseases 1

The Renal Humoral Pressor Mechanism in Man. I. Preparation and Assay of Human Renin, Human Hypertensinogen, and Hypertensin 12

“…The shorter period of incubation has the theoretical advantage of avoiding the destructive action of plasma hypertensinase on the hypertensin formed. Actually, however, the amount of hypertensinase in nonhemolyzed human plasma has no appreciable 'action on hypertensin in a 20-minute incubation period at 370 C. and our values have been almost identical with theirs (32,33). The only advantage of our method over theirs is that it requires the use of only an eighth the amount of human renin.…”

“…In the establishment of absolute values for the concentration of hypertensinogen in plasma of human beings, a fairly wide range of values has been obtained by this method by both ourselves (32,33) and the above mentioned workers (22), in part due, we believe, to the inherent difficulties of the method already discussed and in part to normal variations in titer. In order to obviate as much as possible errors in interpretation in such a study, it has been our custom to test each plasma on several occasions in the same or different assay animal, and to compare the values of pathological plasmas with that of a normal control subject.…”