The Renal Humoral Pressor Mechanism in Man. I. Preparation and Assay of Human Renin, Human Hypertensinogen, and Hypertensin 12

Dexter, Lewis; Haynes, Florence W.; Bridges, William C.

doi:10.1172/jci101579

Cited by 34 publications

(11 citation statements)

References 15 publications

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“…of nonhemolyzed human plasma were incubated for 25 minutes, at 370 C., at pH 7.3, with an excess of human renin, as described in detail elsewhere (4). The hypertensin formed was assayed on cats by comparing the blood pressure rise obtained from the sample tested with that of a standard hypertensin solution.…”

Section: Methodsmentioning

confidence: 99%

The Renal Humoral Pressor Mechanism in Man. Iv. The Hypertensinogen Content of the Plasma of Normal Patients and Patients With Various Diseases 1

Haynes¹,

Dexter²

1945

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

The Renal Humoral Pressor Mechanism in Man. Iv. The Hypertensinogen Content of the Plasma of Normal Patients and Patients With Various Diseases 1

Haynes¹,

Dexter²

1945

J. Clin. Invest.

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“…Preparation of Renin.--The renin was prepared from fresh hog kidneys according to the method described by Katz and Goldblatt (5) and modified by Dexter (6). Tiffs preparation contained a considerable amount of hypertensinase activity which was eliminated (reference 7, page 101) in the following fashion.…”

Section: Metkodsmentioning

confidence: 99%

“…This was done using a dialyzed non-fractionated human plasma as the substrate preparation and human renin prepared according to the method of Dexter (6). The human hypertensin prepared by the salt-free incubation of these materials gave a distribution pattern similar to that of horse hypertensin I.…”

Section: Demonstration Of Hypertensin H--mentioning

confidence: 99%

The Existence of Two Forms of Hypertensin

Skeggs

Marsh

Kahn

et al. 1954

Journal of Experimental Medicine

407

111

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The pressor substance hypertensin, which is the product of the action of the renal enzyme renin upon its plasma substrate, has been shown to be present in the arterial blood of many patients with hypertensive cardiovascular disease (1) as well as in the arterial blood of animals with experimental renal hypertension (2--4). Hypertensin may, therefore, be concerned in causing the elevation of blood pressure in both of these conditions. In the course of attempts to purify this material it was found that certain preparations, when examined by means of the countercurrent distribution method, revealed not one but two pressor substances. Further study of this completely unexpected finding showed that the initial pressor substance, hypertensin I, formed as a result of the action of renin on its substrate, is quickly converted to a second substance with approximately equal pressor activity, hypertensin II, apparently by an enzyme in plasma, which is activated by chloride ion. I t is the purpose of this paper to describe the preparation of hypertensin I from suitable preparations of renin and substrate, to demonstrate its conversion to hypertensin I I by the action of the plasma enzyme, and to determine which ions are effective in activating this enzyme. MetkodsPreparation of Renin.--The renin was prepared from fresh hog kidneys according to the method described by Katz and Goldblatt (5) and modified by Dexter (6). Tiffs preparation contained a considerable amount of hypertensinase activity which was eliminated (reference 7, page 101) in the following fashion. Renin, prepared from 100 pounds of hog kidneys by ammonium sulfate precipitation, was dissolved in 1.5 liters of water. The solution was chilled to 0-5°C. and sufficient solid sodium chloride was added to insure complete saturation. 0.1 N HCI, saturated with NaC1, wa~nthen dripped into the solution slowly and with constant stirring until the pH dropped to 2.0. The solution was centrifuged at high speed at 0-5°C. The supernatant containing the hypertensinase was decanted and discarded. The precipitate was dissolved in 1 liter of cold 0.1 ~ Na,I-IPO4. The light brown solution was adjusted to pH 7.5, put into small diameter cellophane bags, and dialyzed exhaustively against cold distilled water. The resulting product, with a volume of about 1.25 liters, was found to be uniformly free of hypertensinase. Usually 1 volume of this enzyme preparation was adequate to catalyze the formation of hypertensin in 25 volumes of the substrate preparation described below in a period of 30 to 45 minutes when this mixture was incubated at 37°C. and pH 7.5.Preparation of Substrate.--Horse blood was collected in ~0 volume of 4 per cent sodium

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“…In the last 2 cases, however, and in all the animal experiments, the renin concentration of 5 to 10 ml. of plasma was determined by the direct method (13), modified (14). The specificity of the methods was indicated by a pressor response from the tube which had been incubated, while essentially no change in blood pressure resulted from injection of the unincubated tube.…”

Section: Methodsmentioning

confidence: 99%

The Renal Humoral Pressor Mechanism in Man. Ii. The Effect of Transitory Complete Constriction of the Human Renal Artery on Blood Pressure and on the Concentration of Renin, Hypertensinogen, and Hypertensinase of Renal Arterial and Venous Blood, With Animal Observations 1

Quinby¹,

Dexter²,

Sandmeyer³

et al. 1945

J. Clin. Invest.

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The Renal Humoral Pressor Mechanism in Man. I. Preparation and Assay of Human Renin, Human Hypertensinogen, and Hypertensin 12

Cited by 34 publications

References 15 publications

The Renal Humoral Pressor Mechanism in Man. Iv. The Hypertensinogen Content of the Plasma of Normal Patients and Patients With Various Diseases 1

The Renal Humoral Pressor Mechanism in Man. Iv. The Hypertensinogen Content of the Plasma of Normal Patients and Patients With Various Diseases 1

The Existence of Two Forms of Hypertensin

The Renal Humoral Pressor Mechanism in Man. Ii. The Effect of Transitory Complete Constriction of the Human Renal Artery on Blood Pressure and on the Concentration of Renin, Hypertensinogen, and Hypertensinase of Renal Arterial and Venous Blood, With Animal Observations 1

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