The repeated open application test: suggestions for a scale of evaluation

Use tests: ROAT (repeated open application test)/PUT (provocative use test): an overview

“…A difference in the morphological assessment of use test and patch test reactions is also reported (12). We require a standardized measurement of the results of use testing like the ICDRG scoring system for patch testing (13).…”

Suspected allergic contact dermatitis to iodopropynyl butylcarbamate in an alcohol hand rub commonly used in Australian health-care settings

“…Only 18 of the 36 tests produced a reaction. 7 It is plausible that our patient's use of Microshield Angel on her hands resulted in sufficient exposure to IPBC to meet the elicitation threshold, however ROAT testing did not.…”

Oral Presentations

“…7 patients had simultaneous reactions to TDM, PPD and BRM. 17/47 patients reacted positively to DO 1, 8 reacted to DO 3,6 to DB 124 and DR1 respectively, 5 to DB 106 and DR 17 respectively, 3 to DB 35, and 1 to DO 3. Simultaneous reactions were seen especially between DO1 and PPD and/or BRM.…”

“…Testing with more than one allergen concentration -together with patient history, clinical picture and known exposures -may help improve our interpretation of the clinical relevance of patch test results. Serial dilution testing and 'use tests' as the Repeated Open Application Test (ROAT) on normal as well as on dermatitic skin are important tools in the relevance evaluation process challenging both the dermatologist and patient [6,7] . The elicitation of a positive patch test reaction in a given individual depends upon: (i) the dose, i.e., the number of molecules of the sensitizer applied; (ii) the patch test technique, i.e.…”