The repertoire of T-lymphocytes recovered by bronchoalveolar lavage from healthy nonsmokers

Burastero, SE; Borgonovo, Barbara; Gaffi, Davide; Frittoli, Emanuela; Wack, Andreas; Rossi, G. A.; Crimi, Emanuele

doi:10.1183/09031936.96.09020319

Cited by 24 publications

(20 citation statements)

References 24 publications

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“…In line with this finding are the results of BURASTERO et al [158] who analysed the TCR V β pattern of T-cell clones generated from BAL and peripheral blood and found no significant difference in the TCR V β usage in these two body compartments. Thus, the analysis of the TCR V β pattern of T-cell clones from sarcoidosis patients will provide insight into immune mechanisms taking place in body compartments other than blood or BAL.…”

Section: T-cellssupporting

confidence: 57%

Sarcoidosis: immunopathogenetic concepts and their clinical application

1998

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Our understanding of the immunopathogenesis of sarcoidosis has been advanced by studies of bronchoalveolar lavage cells. Activated macrophages and T-cells have been identified in different compartments of the sarcoid lung and the characteristics of the activation suggest that the cells become activated in the course of a normal immune response. The immune cells communicate via a cytokine network and the measuring of cytokine levels yields subgroups of sarcoidosis patients with different courses of the disease indicating different states of activation of the disease-mediating immune cells. The causative agent of sarcoidosis has not yet been identified; however, some of the described mechanisms can be clinically applied either to detect patients at risk of deterioration or to develop new therapeutic strategies. Using these approaches methotrexate, pentoxifylline and thalidomide have been identified as drugs which effectively suppress sarcoid inflammation and the serum level of soluble interleukin-2 receptors has been delineated to be a serum marker of sarcoid inflammation. Furthermore, analysing the pulmonary cytokine network in sarcoidosis will yield new staging parameters possibly supplying prognostic information and guiding therapeutic intervention.

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Section: T-cellssupporting

confidence: 57%

Sarcoidosis: immunopathogenetic concepts and their clinical application

1998

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“…This is in contrast to the findings in healthy subjects, where T cell expansions in the peripheral blood are common in the CD8ϩ T cell population but not in the CD4ϩ T cell population and where BV3 is found on Ͻ5% of CD4ϩ T cells (22). Data on the T cell repertoire in BAL fluid from healthy individuals are scarce, but suggest a similar profile of T cells in BAL fluid as in peripheral blood (29,30).…”

Section: Discussionmentioning

confidence: 99%

Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies

Englund¹,

Wahlström²,

Fathi³

et al. 2007

Arthritis & Rheumatism

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Objective. To investigate T cell receptor (TCR) expression in 3 different compartments that could be involved in patients with myositis: muscle, lung, and peripheral blood.Methods. Nine patients with polymyositis (PM), dermatomyositis, or inclusion body myositis underwent bronchoscopy and bronchoalveolar lavage (BAL) as well as muscle biopsy and blood sampling. A panel of 19 monoclonal antibodies specific for TCR V ␤ (BV) and V ␣ (AV) were used to characterize the TCR profile in CD4؉ and CD8؉ T cell populations in BAL fluid and peripheral blood by flow cytometry. Muscle biopsy tissues were analyzed by immunohistochemistry. Patients were also typed for HLA-DRB1 and DRB3 alleles.Results. A total of 17 T cell expansions were detected in BAL fluid, 6 in the CD4؉ T cell population and 11 in the CD8؉ T cell population. Four T cell expansions were detected in peripheral blood. A selective TCR V usage was found in muscle. Two PM patients, both of whom had BAL fluid BV3؉ T cell expansions in the CD4 population and in whom BV3 was also a prominent TCR V segment in muscle tissue, shared the HLA-DRB1*03 allele. These 2 patients were the only ones who were positive for anti-Jo-1 antibody.Conclusion. We found a restricted accumulation of T lymphocytes expressing selected TCR V-gene segments in the target organ compartments (i.e., lung and muscle). The occurrence of shared TCR gene segment usage in muscle and lungs could suggest common target antigens in these organs.

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“…The remaining 20 µl of PCR-amplified products were subsequently run on a 12% native polyacrylamide gel electrophoresis (PAGE) as previously described [28, 29, 32]. Briefly, 20 µl of each amplified fragment was heated at 94°C for 5 min to denature DNA, re-annealed for 1 h at 50°C, and kept at 4°C until gel loading.…”

Section: Methodsmentioning

confidence: 99%

“…This temperature is permissive for re-annealing between DNA strains with minor mismatches (heteroduplexes) as well as between homologous strains (homoduplexes). Gene fragments differing for single-point mutations in the N region are detected with this assay [32]. Vβ-amplified fragments derived from grass and birch T-cell lines were run in parallel lanes.…”

Section: Methodsmentioning

confidence: 99%

T-Cell Receptor-Mediated Cross-Allergenicity

Burastero

Paolucci

Breda

et al. 2004

Int Arch Allergy Immunol

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Background: Profilins are conserved and ubiquitous plant and animal proteins. We wanted to discover whether the T-cell response to conserved epitopes on birch and grass profilins could account for cross-allergenicity in subjects allergic to these two pollens. Methods: Thirty-one patients allergic to grass and birch were recruited for the study. Grass and birch reactive T lymphocytes were studied by measuring proliferation to birch and grass allergen, respectively, followed by Vβ T-cell receptor family-specific polymerase chain reaction and heteroduplex analysis. T-cell clones were derived from patients with cross-proliferating T cells. Results: In 25 of 31 subjects the T-cell response to grass was quite distinct from that to birch. In contrast, in 6 of 31 individuals grass T cells cross-proliferated to birch and this was reproduced in 4 patients by birch profilin. CD4 Th2 cell clones were derived which promiscuously recognized homologously conserved regions on birch and grass profilins. Conclusion: We conclude that a functionally relevant T-cell response to conserved regions of panallergens underlie cross-allergenicity in a subset of allergic patients. These results suggest that a reciprocal modulation of the response to one sensitizing allergen can occur following natural exposure to or immunotherapy with another allergen. These results have relevance in the management of patients with multiple allergies.

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The repertoire of T-lymphocytes recovered by bronchoalveolar lavage from healthy nonsmokers

Cited by 24 publications

References 24 publications

Sarcoidosis: immunopathogenetic concepts and their clinical application

Sarcoidosis: immunopathogenetic concepts and their clinical application

Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies

T-Cell Receptor-Mediated Cross-Allergenicity

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