The resistance to leaf rust and powdery mildew of recombinant lines of barley (Hordeum vulgare L.) derived from H. vulgare × H. bulbosum crosses

Abstract

A set of 23 recombinant lines (RLs) of barley (Hordeum vulgare L.) derived from H. vulgare · H. bulbosum L. crosses was inoculated with barley leaf rust (Puccinia hordei) and powdery mildew (Blumeria graminis f.sp. hordei) at the seedling stage to identify their levels and mechanisms of resistance. Eight RLs were studied further in glasshouse and field tests. All three barley parents (ÔEmirÕ, ÔGolden PromiseÕ and ÔVadaÕ) were highly susceptible to powdery mildew and leaf rust isolates. Several RLs showed partial resistance expressed as high relative latency periods and low relative infection frequencies against leaf rust. This high level of partial resistance was due to a very high level of early aborting colonies without host cell necrosis. Several RLs showed hypersensitive resistance to some or all isolates. For powdery mildew, one RL was completely resistant to the CC1 isolate and had a hypersensitive resistance to the CO-02 isolate. Three RLs derived from ÔEmirÕ were completely resistant to both powdery mildew isolates, and three more RLs tested in the field had higher levels of partial resistance than their parents. The results indicate that H. bulbosum contains major and minor gene(s) for resistance to leaf rust and powdery mildew that can be transferred to cultivated barley.

Genes for resistance to powdery mildew in European barley cultivars registered in the Czech Republic from 2011 to 2015

“…, Shtaya et al. ), could also provide an alternative source, although increasing infection of the intergeneric wheat–rye hybrid triticale with powdery mildew (Walker et al. , Klocke et al.…”

Virulence frequency to powdery mildew resistances in winter barley cultivars

“…In contrast with most of the other host-pathogen systems, in the barley-powdery mildew pathogen interaction there are a lot of resistance genes, many others have been more or less described Řepková et al 2006, 2008 and a large number of new genes, particularly in wild barleys (Hordeum vulgare ssp. spontaneum and Hordeum bulbosum), can be anticipated (Dreiseitl & Bockelman 2003;Dreiseitl & Dinoor 2004;Shtaya et al 2007). …”

Marker assisted separation of resistance genes Rph22 and Rym16 Hb from an associated yield penalty in a barley: Hordeum bulbosum introgression line

“…Hordeum bulbosum L. is a member of the secondary gene pool of cultivated barley (Hordeum vulgare L.) and has been used mostly in barley improvement as a means to produce doubled haploids through chromosome elimination (Kasha and Kao 1970). In addition, H. bulbosum is considered a non-host to many pathogens which are virulent upon barley, and several resistance genes have been successfully introgressed from H. bulbosum into cultivated barley (Fetch et al 2009;Johnston et al 2013;Pickering et al 1995Pickering et al , 1998Pickering et al , 2000Pickering et al , 2006Ruge-Wehling et al 2006;Ruge et al 2003;Scholz et al 2009;Shtaya et al 2007;Toubia-Rahme et al 2003;Walther et al 2000;Xu and Kasha 1992). The development of crops which are resistant to diseases is a major goal of plant breeding in almost all commercial crop species.…”