The Response of Anther Culture in a Genetically Wide Material of Winter Wheat (Triticum aestivum L.)

Abstract

In a screening experiment with 215 different winter cukivars of Triticum aestivum L. for response in anther culture, pollen embryos or callus were obtained from 200 different cukivars and green plants from 93 different cultivars. On average, from the whole material, 1.3 green plants were obtained per 100 anthers cultured. Variance components estimated from replicates with selected parts of the plant material indicated that for the formation of pollen embryos from anthers, interactions between genotypes and replications were dominating, accounting for 45 to 50 per cent of the variation. Main effects from genotypes were less prominent, accounting for 25 to 30 per cent of the variation, and the replications showed relativel)' little effect, accounting for about 4 to 12 per cent of total variation. Regeneration of plants from pollen embrj'os or callus was not significantly mfluenced by either genotype or replicates. The frequencies of plants regenerated being green, however, were influenced from hoth genotype and environments contributing 42.5 and 34.9 per cent of the total variation respectively, while only minor interactions between genotype and environments were indicated for this character.

Genetic Mapping of QTLs for Tissue-Culture Response in Plants

“…useful for genetic analysis, particularly for mapping of quantitative traits El Attari et al, 1998;Caranta et al, 1997;Mahmood et al, 2003). Anther culture response is known to be under a strong genotypic influence (Rakoczy-Trojanowska et al, 1997;Reddy et al, 1985;Andersen et al, 1987;Petolino & Jones, 1986). Thus, when DH lines are produced, some cross combinations show a low efficiency in anther culture and some show segregation distortion in their DH lines.…”

Improving green plant production via isolated microspore culture in bread wheat (Triticum aestivum L.)

“…Genotypic differences in androgenesis induction do exist (Anderson et al 1987;Tuvesson et al 1989). Some genotypes naturally react better than others in response to environmental changes, such as stress treatments applied to microspores.…”

Reciprocal effects in anther cultures of wheat hybrids

“…Most of the studies on the inheritance of anther culture response in wheat have focused only on nuclear genetic control and have not examined the reciprocal effects (Becraft and Taylor 1992, Deaton et al 1987, Agache et al 1989, Moinei et al 1997. Tuvesson et al (1989) andChaudhery et al (2003) reported that maternal and cytoplasmic effects are not important for anther culture response.…”