The Response of Matrix Metalloproteinase-9 and -2 to Exercise

Nascimento, Dahan da Cunha; Marqueti, Rita de Cássia; Tibana, Ramires Alsamir; Durigan, João Luiz Quagliotti; Navalta, James W.; Prestes, Jonato

doi:10.1007/s40279-014-0265-8

Cited by 38 publications

(27 citation statements)

References 49 publications

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“…Motility of cancer cells is associated with its invasiveness through extracellular matrix. Metastasis of human HCC cells may request both high motility of HCC cell as well as the ability of tumor cell in degenerating extracellular matrix [ 34 , 35 , 36 ]. To further investigate the possible anti-metastasis effect of berberine in HCC cells, we conducted a transwell chamber assay to observe the effect of berberine on invasiveness of tumor cell through extracellular matrix.…”

Section: Resultsmentioning

confidence: 99%

“…In this case, the initiating and essential step of tumor cell invasion is to break through from the restriction of extracellular matrix by degrading local proteins. The most important executors for proteolysis are MMPs [ 34 , 35 , 36 ]. Furthermore, such process is controlled by some signaling pathways including PAI-1, tPA, uPA and TIMPs [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

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Up-Regulation of PAI-1 and Down-Regulation of uPA Are Involved in Suppression of Invasiveness and Motility of Hepatocellular Carcinoma Cells by a Natural Compound Berberine

Wang

et al. 2016

IJMS

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Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death and its prognosis remains poor due to the high risk of tumor recurrence and metastasis. Berberine (BBR) is a natural compound derived from some medicinal plants, and accumulating evidence has shown its potent anti-tumor activity with diverse action on tumor cells, including inducing cancer cell death and blocking cell cycle and migration. Molecular targets of berberine involved in its inhibitory effect on the invasiveness remains not yet clear. In this study, we identified that berberine exhibits a potent inhibition on the invasion and migration of HCC cells. This was accompanied by a dose-dependent down-regulation of expression of Cyclooxygenase-2 (COX-2), nuclear factor kappa B (NF-κB), urokinase-type plasminogen activator (uPA) and matrix metalloproteinase (MMP)-9 in berberine-treated HCC cells. Furthermore, berberine inactivated p38 and Erk1/2 signaling pathway in HCC cells. Primarily, this may be attributed to the up-regulation of plasminogen activator inhibitor-1 (PAI-1), a tumor suppressor that can antagonize uPA receptor and down-regulation of uPA. Blockade of uPA receptor-associated pathways leads to reduced invasiveness and motility of berberine-treated HCC cells. In conclusion, our findings identified for the first time that inactivation of uPA receptor by up-regulation of PAI-1 and down-regulation of uPA is involved in the inhibitory effect of berberine on HCC cell invasion and migration.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Up-Regulation of PAI-1 and Down-Regulation of uPA Are Involved in Suppression of Invasiveness and Motility of Hepatocellular Carcinoma Cells by a Natural Compound Berberine

Wang

et al. 2016

IJMS

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“…One more probable mechanism is the decrement of TNF-α induced by the training, a well-known exciter of MMP-9 production. Results revealed that chronic aerobic training leads to cardio protective effects and is useful for the prevention of diabetes, hypertension, cancer, osteoporosis, and dementia (20).…”

Section: Discussionmentioning

confidence: 99%

Effect of Continuous Endurance Training on mRNA Expression of Interferon Gamma and Matrix Metalloproteinase-9 in Male Elder Rats

Mousavi

Asad

Barzegari

et al. 2018

Hormozgan medical journal

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Background: Aging impairs function of the heart and is associated with mechanical remodeling. This process involves accumulation of collagen and dysfunction in the regulation of active matrix metalloproteinase. On the other hand, exercise training improves cardiac function and regulates the increase of pro-inflammatory molecules, such as interferon gamma, yet the impact of exercise training on aging has not yet been defined. Objectives: This study examined the effect of continuous endurance training on mRNA expression of interferon gamma (IFN-γ) and matrix metalloproteinase-9 (MMP-9) in male elder rats. Methods: For this purpose, 14 Wistar male rats with age of 24 to 26 months and an average weight of 380 ± 20 g were randomly divided to two groups, including training (n = 7) and control (n = 7). Training groups performed continuous endurance training for six weeks. Matrix metalloproteinase-9 mRNA and interferon (IFN)-γ mRNA expression in heart tissue were measured by real-time polymerase chain reaction. To analyze of the data, the independent t-test was used at a significance level of P < 0.05. Results: The findings of this statistical analysis showed that six weeks of continuous endurance training had a significant effect on MMP-9 mRNA expression (P = 0.001). Also, this training protocol did not have a significant effect on expression of IFN-γ gene (P = 0.628). Conclusions: Continuous endurance training attenuated aging-induced cardiac inflammation of rats. There are numerous questions that remain to be answered to complete the understanding of the moderator effects of exercise on MMP-9 and IFN-γ, as a muscle conformity or indicate an inflammatory condition.

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“…Previous human studies have demonstrated that physical exercise can affect the levels of MMPs following acute and chronic interventions. Under coronary risk events, the elevations of MMP-2 and MMP-9 are correlated with increased inflammation [ 21 ]. The decrease in MMPs could be related to expression of the tissue inhibitors of metalloproteinases (TIMPs), protease degradation, and reduction of proinflammatory cytokine tumor necrosis factor-alpha (TNF- α ) [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Cardioprotective Effects of Voluntary Exercise in a Rat Model: Role of Matrix Metalloproteinase-2

Pósa

Szabó

Kupai

et al. 2015

Oxidative Medicine and Cellular Longevity

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Background. Regular exercise at moderate intensity reduces cardiovascular risks. Matrix metalloproteinases (MMPs) play a major role in cardiac remodeling, facilitating physiological adaptation to exercise. The aim of this study was to examine the influence of voluntary physical exercise on the MMP-2 enzyme activity and to investigate the cardiac performance by measurement of angina susceptibility of the heart, the basal blood pressure, the surviving aorta ring contraction, and the cardiac infarct size after I/R-induced injury. Methods. Male Wistar rats were divided into control and exercising groups. After a 6-week period, the serum level of MMP-2, basal blood pressure, cardiac angina susceptibility (the ST segment depression provoked by epinephrine and 30 s later phentolamine), AVP-induced heart perfusion and aorta ring contraction, infarct size following 30 min ischemia and 120 min reperfusion, and coronary effluent MMP-2 activity were measured. Results. Voluntary wheel-running exercise decreased both the sera (64 kDa and 72 kDa) and the coronary effluent (64 kDa) MMP-2 level, reduced the development of ST depression, improved the isolated heart perfusion, and decreased the ratio of infarct size. Conclusion. 6 weeks of voluntary exercise training preserved the heart against cardiac injury. This protective mechanism might be associated with the decreased activity of MMP-2.

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The Response of Matrix Metalloproteinase-9 and -2 to Exercise

Cited by 38 publications

References 49 publications

Up-Regulation of PAI-1 and Down-Regulation of uPA Are Involved in Suppression of Invasiveness and Motility of Hepatocellular Carcinoma Cells by a Natural Compound Berberine

Up-Regulation of PAI-1 and Down-Regulation of uPA Are Involved in Suppression of Invasiveness and Motility of Hepatocellular Carcinoma Cells by a Natural Compound Berberine

Effect of Continuous Endurance Training on mRNA Expression of Interferon Gamma and Matrix Metalloproteinase-9 in Male Elder Rats

Cardioprotective Effects of Voluntary Exercise in a Rat Model: Role of Matrix Metalloproteinase-2

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