An important role in increasing the production of high-protein feed in the steppe zone belongs to leguminous grasses, such as alfalfa, sainfoin, and melilot. The value of perennial grasses, including alfalfa, is not only in providing high-protein feeds and their productivity in the sharply continental climate of Northern Kazakhstan, but it also has great importance in increasing soil fertility. The purpose of this research was to study the influence of biological preparations on the germination, growth, and development of alfalfa of the Kokshe variety in the conditions of the hill and plain zone of the Akmola region of Kazakhstan. The field experiments were conducted in 2020-2022 in the Kokshetau experimental production farm LLP. The following biological preparations for seed treatment were used: BioSleep BW + Foliar (1 l/ha), Orgamica S + Foliar (1 l/ha), Organit P, Organit N, Biodux + Foliar (2 l/ha) and Foliar. The seed treatment with biological preparations was carried out 12 h before sowing, after which the seeds were dried. The Organit P + Organit N + Biodux + Foliar variant showed the highest rates of germination energy (70.5%) and laboratory and field germination (85 and 52%, respectively). The use of Biosleep BW + Foliar had the best effect on the height of plants and the productivity of green and dry mass, where the height of plants was 57.2 cm and the productivity of green and dry mass was 121 and 48 g/plant, respectively. Thus, when cultivating alfalfa of the Kokshe variety for seeds using elements of organic technology, it is recommended to use Organit P + Organit N + Biodux + Foliar and BioSleep BW + Foliar biological preparations.