The results of unilateral and bilateral extirpation of the forebrain of Amblystoma

Detwiler, S. R.

doi:10.1002/jez.1401000106

Cited by 33 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the highly deterministic pattern of division characteristic of lineage-based mechanisms of phenotypic determination do not appear to apply to retinal histogenesis. This conclusion is consistent with a great deal of experimental embryological studies of the developing CNS, in which particular regions of the embryonic nervous system can be induced to change their commitment or regenerate adjacent areas (Detwiler, 1945(Detwiler, , 1946Harrison, 1947; also see Cowan and Finger, 1982, for a review).…”

Section: Discussionsupporting

confidence: 70%

Age of differentiation determines rat retinal germinal cell phenotype: induction of differentiation by dissociation

1989

View full text Add to dashboard Cite

We are interested in the mechanisms that control cell phenotype during the development of the CNS. Since different neuronal types arise at different times during neurogenesis in the retina, we predicted that the factors that determine cell type must be developmentally regulated as well. To test this hypothesis, we induced retinal germinal cells to differentiate at different ages by dissociating the retina into single cells and culturing them on a variety of substrates. Prior to dissociation, the S-phase germinal cells were labeled with 3H-thymidine so that their fate could be specifically followed. We found that our culture conditions promoted the differentiation of the majority of the germinal cells and that these cells differentiated into different neuronal types depending on the age of the animal from which the retina had been taken; embryonic day 14 germinal cells differentiated primarily into ganglion cells, and never produced rods, while germinal cells from postnatal day 1 retina differentiated into rods, but not ganglion cells. These results are consistent with the hypothesis that temporally regulated factors determine cell phenotype during the development of the retina.

show abstract

Section: Discussionsupporting

confidence: 70%

Age of differentiation determines rat retinal germinal cell phenotype: induction of differentiation by dissociation

1989

View full text Add to dashboard Cite

show abstract

“…It also appears before reaction to stimulation in fishes and birds. Detwiler (1946) found that in young larval amblystoma swimming movements were perfectly executed after transection below the auditory vesicle, controlled by the lower medulla and spinal cord. Subsequent to a later stage of development sustained motor activity, including swimming, was found to fail rapidly if the influence of the midbrain was eliminated.…”

Section: Chronic Lesion Of Cerebellar Vermis and Tectum After Bilateral Corticospinal Lesionmentioning

confidence: 94%

The Midbrain and Motor Integration

Denny‐Brown¹

1962

Proceedings of the Royal Society of Medicine

128

View full text Add to dashboard Cite

show abstract

“…Whereas the reconstitution of various embryonic parts of the central nervous system after their ablation is a widespread phenomenon (Lewis, 1906;Bell, 1906Bell, , 1907Spemann, 1912;Burr, 1916;Detwiler, 1944Detwiler, , 1945Detwiler, , 1946Detwiler, , 1947Harrison, 1947;Birge, 1959;Martinez and Alvardo-Mallart, 1989;D. Dutting, personal communication; for an excellent review see Cowan and Finger, 1982), in adult vertebrates this capacity is much more restricted to certain parts of certain species.…”

Section: Introductionmentioning

confidence: 99%

A Hidden Retinal Regenerative Capacity from the C Ciliary Margin is Reactivated In Vitro, that is Accompanied by Down‐regulation of Butyrylcholinesterase

Willbold

Layer

1992

Eur J of Neuroscience

View full text Add to dashboard Cite

The chicken retina has a capacity to regenerate in vivo, which is restricted up to embryonic day 4 (E4). Here we test the proliferative patterns of dissociated chicken cells from the centre retina or the ciliary margin, including pigmented cells, after their transfer into rotation culture. For central cells in culture, the uptake of [3H]thymidine after a short initial rise decreases similarly to their in ovo counterparts. In contrast, marginal cells that have been shown to regenerate up to E9 into retinotypic stratospheroids re-enter a novel and long-lasting phase of in vitro cell division. We have shown previously that cell types of all nuclear layers are produced. Both observations taken together indicate a pronounced self-renewal of multipotent stem cells. Molecularly, the enzyme butyrylcholinesterase, which in other systems has been shown to mark transitory neuronal cells between proliferation and differentiation, is strongly expressed at the ciliary margin over most of the embryonic period. After these cells are transferred into rotation culture, butyrylcholinesterase is down-regulated. Concomitantly, the neuronal differentiation marker acetylcholinesterase increases. We conclude that the regenerative capacity of the chick retina is not lost at E4, but rather remains hidden in the chicken ciliary margin, since it can be reactivated in vitro at least up to E9. We suggest that butyrylcholinesterase may be linked to the regulation of stem cell activity.

show abstract

The results of unilateral and bilateral extirpation of the forebrain of Amblystoma

Cited by 33 publications

References 14 publications

Age of differentiation determines rat retinal germinal cell phenotype: induction of differentiation by dissociation

Age of differentiation determines rat retinal germinal cell phenotype: induction of differentiation by dissociation

The Midbrain and Motor Integration

A Hidden Retinal Regenerative Capacity from the C Ciliary Margin is Reactivated In Vitro, that is Accompanied by Down‐regulation of Butyrylcholinesterase

Contact Info

Product

Resources

About