The RhoGEF protein Plekhg5 regulates medioapical and junctional actomyosin dynamics of apical constriction during <i>Xenopus</i> gastrulation

Baldwin, Austin T.; Popov, Ivan K.; Keller, Ray; Wallingford, John B.; Chang, Chenbei

doi:10.1091/mbc.e22-09-0411

Cited by 2 publications

(1 citation statement)

References 62 publications

(91 reference statements)

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“…A more recent study described “cellular constriction chains” (CCCs) that have been associated with positive feedback regulation during propagation of tensile stresses 34 . Whether the morphological changes observed in our study are subject to mechanical, transcriptional or post-transcriptional regulation, they likely play critical roles in many diverse tissues and processes, including Drosophila ventral furrow formation 62 or Xenopus blastopore 63 .…”

Section: Discussionmentioning

confidence: 93%

Mechanical control of neural plate folding by apical domain alteration

Matsuda,

Rozman,

Ostvar

et al. 2023

Nat Commun

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Vertebrate neural tube closure is associated with complex changes in cell shape and behavior, however, the relative contribution of these processes to tissue folding is not well understood. At the onset of Xenopus neural tube folding, we observed alternation of apically constricted and apically expanded cells. This apical domain heterogeneity was accompanied by biased cell orientation along the anteroposterior axis, especially at neural plate hinges, and required planar cell polarity signaling. Vertex models suggested that dispersed isotropically constricting cells can cause the elongation of adjacent cells. Consistently, in ectoderm, cell-autonomous apical constriction was accompanied by neighbor expansion. Thus, a subset of isotropically constricting cells may initiate neural plate bending, whereas a ‘tug-of-war’ contest between the force-generating and responding cells reduces its shrinking along the body axis. This mechanism is an alternative to anisotropic shrinking of cell junctions that are perpendicular to the body axis. We propose that apical domain changes reflect planar polarity-dependent mechanical forces operating during neural folding.

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Section: Discussionmentioning

confidence: 93%

Mechanical control of neural plate folding by apical domain alteration

Matsuda,

Rozman,

Ostvar

et al. 2023

Nat Commun

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Architecture of the cortical actomyosin network driving apical constriction in C. elegans

Zhang

Medwig-Kinney

Goldstein

2023

Journal of Cell Biology

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Apical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving apical constriction are primarily generated through the contraction of apicolateral and/or medioapical actomyosin networks. In the Drosophila ventral furrow, the medioapical actomyosin network has a sarcomere-like architecture, with radially polarized actin filaments and centrally enriched non-muscle myosin II and myosin activating kinase. To determine if this is a broadly conserved actin architecture driving apical constriction, we examined actomyosin architecture during C. elegans gastrulation, in which two endodermal precursor cells internalize from the surface of the embryo. Quantification of protein localization showed that neither the non-muscle myosin II NMY-2 nor the myosin-activating kinase MRCK-1 is enriched at the center of the apex. Further, visualization of barbed- and pointed-end capping proteins revealed that actin filaments do not exhibit radial polarization at the apex. Our results demonstrate that C. elegans endodermal precursor cells apically constrict using a mixed-polarity actin filament network and with myosin and a myosin activator distributed throughout the network. Taken together with observations made in other organisms, our results demonstrate that diverse actomyosin architectures are used in animal cells to accomplish apical constriction.

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The RhoGEF protein Plekhg5 regulates medioapical and junctional actomyosin dynamics of apical constriction during Xenopus gastrulation

Cited by 2 publications

References 62 publications

Mechanical control of neural plate folding by apical domain alteration

Mechanical control of neural plate folding by apical domain alteration

Architecture of the cortical actomyosin network driving apical constriction in C. elegans

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