The ribosomal protein genes and Minute loci of Drosophila melanogaster

Marygold, Steven J; Roote, John; Reuter, Günter; Lambertsson, Andrew; Ashburner, Michael; Millburn, Gillian; Harrison, Paul M.; Ye, Zhan; Kenmochi, Naoya; Kaufman, Thomas C.; Leevers, Sally J.; Cook, Kevin R.

doi:10.1186/gb-2007-8-10-r216

Cited by 363 publications

(389 citation statements)

References 149 publications

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“…Differential growth can also be introduced by creating a mosaic between wild-type cells and slowgrowing cells. This can be done in Drosophila using Minute mutations, which are mutations in genes required for ribosome function that cause a dominant slow-growth phenotype (29). Moreover, because Minute mutations simply reduce the capacity for protein synthesis, they are not expected to directly increase myosin levels.…”

Section: Growth Transcriptionmentioning

confidence: 99%

Differential growth triggers mechanical feedback that elevates Hippo signaling

Pan

Heemskerk

Ibar

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

141

147

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Mechanical stress can influence cell proliferation in vitro, but whether it makes a significant contribution to growth control in vivo, and how it is modulated and experienced by cells within developing tissues, has remained unclear. Here we report that differential growth reduces cytoskeletal tension along cell junctions within faster-growing cells. We propose a theoretical model to explain the observed reduction of tension within faster-growing clones, supporting it by computer simulations based on a generalized vertex model. This reduced tension modulates a biomechanical Hippo pathway, decreasing recruitment of Ajuba LIM protein and the Hippo pathway kinase Warts, and decreasing the activity of the growth-promoting transcription factor Yorkie. These observations provide a specific mechanism for a mechanical feedback that contributes to evenly distributed growth, and we show that genetically suppressing mechanical feedback alters patterns of cell proliferation in the developing Drosophila wing. By providing experimental support for the induction of mechanical stress by differential growth, and a molecular mechanism linking this stress to the regulation of growth in developing organs, our results confirm and extend the mechanical feedback hypothesis.Ajuba | tissue mechanics | Drosophila development | cytoskeleton | Hippo

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Section: Growth Transcriptionmentioning

confidence: 99%

Differential growth triggers mechanical feedback that elevates Hippo signaling

Pan

Heemskerk

Ibar

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

141

147

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“…For example, mutations in a number of genes that directly or indirectly affect protein synthesis capacity have effects on body size, development rate, fertility and longevity (Marygold et al 2007;Teleman et al 2005;Syntichaki and Tavernarakis 2006). However, whether or not naturally occurring, heritable and ecological significant variation in protein synthesis capacity exists and is related to fitness or lifehistory traits is an open question.…”

Section: Introductionmentioning

confidence: 99%

Protein synthesis rates in Drosophila associate with levels of the hsr-omega nuclear transcript

Johnson

Carrington

Hallas

et al. 2009

Cell Stress and Chaperones

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Transcripts of the Drosophila hsr-omega gene are known to interact with RNA processing factors and ribosomes and are postulated to aid in co-ordinating nuclear and cytoplasmic activities particularly in stressed cells. However, the significance of these interactions for physiological processes and in turn for whole-organism fitness remains an open question. Because hsr-omega's cellular expression characteristics suggest it may influence protein synthesis, and because both genotypic and expression variation of hsr-omega have been associated with thermotolerance, we characterised 30 lines for variation in the rates of protein synthesis, measured in ovarian tissues, both before and after a mild heat shock, and for basal levels of the two main hsr-omega transcripts, omega-n and omega-c. As expected, the mild heat shock reduced protein synthesis rates. Large variation occurred among lines in levels of omega-n which was negatively associated with rates of basal protein synthesis-a result that supports the model for the cellular function of omega-n. Furthermore, omega-n levels were associated with hsr-omega genotype of the line parents. Little variation occurred among lines for omega-c levels and no associations were detected with protein synthesis or genotype. Since protein synthesis is a fundamental process for growth and development, we characterised the lines for several life-history traits; however, no associations with protein synthesis, omega-n or omega-c levels were detected. Our results are consistent with the idea that natural variation in hsr-omega expression influence rates of protein synthesis in this species.

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“…First, our genome assembly captured 99% (246 of 248) of the core CEGMA genes (15), and 96% of them (239 of 248) were complete. Second, annotation of the cytoplasmic ribosomal protein genes revealed 83 genes, including the full set of 79 cytoplasmic ribosomal proteins (16,17), and 4 duplicated genes (RpS16, RpS23, RpS28, and RpS30) (SI Appendix S1, Table S2). Third, annotation of the 67 nuclear-encoded oxidative phosphorylation genes shows that the L. humile genome assembly is only missing cox7a (SI Appendix S1, Table S2).…”

mentioning

confidence: 99%

Draft genome of the globally widespread and invasive Argentine ant ( Linepithema humile )

Smith¹,

Zimin²,

Holt

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

260

258

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Ants are some of the most abundant and familiar animals on Earth, and they play vital roles in most terrestrial ecosystems. Although all ants are eusocial, and display a variety of complex and fascinating behaviors, few genomic resources exist for them. Here, we report the draft genome sequence of a particularly widespread and well-studied species, the invasive Argentine ant ( Linepithema humile ), which was accomplished using a combination of 454 (Roche) and Illumina sequencing and community-based funding rather than federal grant support. Manual annotation of >1,000 genes from a variety of different gene families and functional classes reveals unique features of the Argentine ant's biology, as well as similarities to Apis mellifera and Nasonia vitripennis . Distinctive features of the Argentine ant genome include remarkable expansions of gustatory (116 genes) and odorant receptors (367 genes), an abundance of cytochrome P450 genes (>110), lineage-specific expansions of yellow/major royal jelly proteins and desaturases, and complete CpG DNA methylation and RNAi toolkits. The Argentine ant genome contains fewer immune genes than Drosophila and Tribolium , which may reflect the prominent role played by behavioral and chemical suppression of pathogens. Analysis of the ratio of observed to expected CpG nucleotides for genes in the reproductive development and apoptosis pathways suggests higher levels of methylation than in the genome overall. The resources provided by this genome sequence will offer an abundance of tools for researchers seeking to illuminate the fascinating biology of this emerging model organism.

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The ribosomal protein genes and Minute loci of Drosophila melanogaster

Abstract: A combined bioinformatic and genetic approach was used to conduct a systematic analysis of the relationship between ribosomal protein genes and Minute loci in Drosophila melanogaster, allowing the identification of 64 Minute loci corresponding to ribosomal genes.

Cited by 363 publications

References 149 publications

Differential growth triggers mechanical feedback that elevates Hippo signaling

Differential growth triggers mechanical feedback that elevates Hippo signaling

Protein synthesis rates in Drosophila associate with levels of the hsr-omega nuclear transcript

Draft genome of the globally widespread and invasive Argentine ant ( Linepithema humile )

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