The rice RCN1/OsABCG5 mutation is associated with root de-velopment in response to nutrient shortage

Ureshi, An-na; Matuda, Shuichi; Ohashi, Emiko; Onishi, Kazumitsu; Takamure, Itsuro; Kato, Kiyoaki

doi:10.3117/plantroot.6.28

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Many rice mutants with short roots have been isolated (Inukai et al ., , ; Jiang et al ., ; Li et al ., ; Jia et al ., ; Zhao et al ., ; Qin et al ., ; Ureshi et al ., ; Zhang et al ., ). They showed different morphologies and anatomies.…”

Section: Discussionmentioning

confidence: 99%

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Normal root elongation requires arginine produced by argininosuccinate lyase in rice

et al. 2014

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SUMMARYPlant roots play an important role in the uptake of water and nutrients, structural support and environmental sensing, but the molecular mechanisms involved in root development are poorly understood in rice (Oryza sativa), which is characterized by a dense fibrous root system. Here we report a rice mutant (red1 for root elongation defect 1) with short roots. Morphological and physiological analyses showed that the mutant had a shorter length from the quiescent center (QC) to the starting point of the elongation zone but a similar cell size and number of lateral and crown roots compared with the wild type. Furthermore, the mutant had similar radial structure and nutrient uptake patterns to the wild type. Map-based cloning revealed that the mutant phenotype was caused by a point mutation of a gene encoding an argininosuccinate lyase (ASL), catalyzing the last step of arginine biosynthesis. The OsASL1 gene has two distinct transcripts, OsASL1.1 and OsASL1.2, which result from different transcription start sites, but only OsASL1.1 was able to complement the mutant phenotype. OsASL1.1 was expressed in both the roots and shoots. The protein encoded by OsASL1.1 showed ASL activity in yeast. OsALS1.1 was localized to the plastid. The short root of the mutant was rescued by exogenous addition of arginine, but not by other amino acids. These results indicate that arginine produced by ASL is required for normal root elongation in rice.

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Section: Discussionmentioning

confidence: 99%

“…osred1 mutant shows a distinct morphological and anatomical phenotype Many rice mutants with short roots have been isolated (Inukai et al, 2001(Inukai et al, , 2005Jiang et al, 2005;Li et al, 2006;Jia et al, 2008;Zhao et al, 2009;Qin et al, 2012;Ureshi et al, 2012;Zhang et al, 2012). They showed different morphologies and anatomies.…”

Section: Discussionmentioning

confidence: 99%

Normal root elongation requires arginine produced by argininosuccinate lyase in rice

et al. 2014

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“…It is likely that altered root permeability affects development of many plants. For example, rice abcg5/rcn1 mutants defective in a closely related transporter also have a defective suberin barrier and altered root and shoot architecture (Yasuno et al, 2009;Ureshi et al, 2012;Shiono et al, 2014).…”

Section: Physiological Importance Of Barriersmentioning

confidence: 99%

ABCG Transporters Are Required for Suberin and Pollen Wall Extracellular Barriers inArabidopsis

et al. 2014

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Effective regulation of water balance in plants requires localized extracellular barriers that control water and solute movement. We describe a clade of five Arabidopsis thaliana ABCG half-transporters that are required for synthesis of an effective suberin barrier in roots and seed coats (ABCG2, ABCG6, and ABCG20) and for synthesis of an intact pollen wall (ABCG1 and ABCG16). Seed coats of abcg2 abcg6 abcg20 triple mutant plants had increased permeability to tetrazolium red and decreased suberin content. The root system of triple mutant plants was more permeable to water and salts in a zone complementary to that affected by the Casparian strip. Suberin of mutant roots and seed coats had distorted lamellar structure and reduced proportions of aliphatic components. Root wax from the mutant was deficient in alkylhydroxycinnamate esters. These mutant plants also had few lateral roots and precocious secondary growth in primary roots. abcg1 abcg16 double mutants defective in the other two members of the clade had pollen with defects in the nexine layer of the tapetum-derived exine pollen wall and in the pollen-derived intine layer. Mutant pollen collapsed at the time of anther desiccation. These mutants reveal transport requirements for barrier synthesis as well as physiological and developmental consequences of barrier deficiency.

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“…The rcn1 (rcn1-2) mutant has a single nucleotide substitution in the gene encoding a member of the ATP-binding cassette (ABC) transporter subfamily G, RCN1/OsABCG5, causing an Ala-684Pro substitution (Yasuno et al, 2009). The mutation results in several mutant phenotypes, although the substrates of RCN1/OsABCG5 have not been determined (Ureshi et al, 2012;Funabiki et al, 2013;Matsuda et al, 2014). We previously found that the rcn1 mutant has abnormal root morphology, such as shorter root length and brownish appearance of roots, under stagnant (deoxygenated) conditions (which mimics oxygen-deficient conditions in waterlogged soils).…”

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Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots

et al. 2015

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In rice (Oryza sativa) roots, lysigenous aerenchyma, which is created by programmed cell death and lysis of cortical cells, is constitutively formed under aerobic conditions, and its formation is further induced under oxygen-deficient conditions. Ethylene is involved in the induction of aerenchyma formation. reduced culm number1 (rcn1) is a rice mutant in which the gene encoding the ATP-binding cassette transporter RCN1/OsABCG5 is defective. Here, we report that the induction of aerenchyma formation was reduced in roots of rcn1 grown in stagnant deoxygenated nutrient solution (i.e. under stagnant conditions, which mimic oxygen-deficient conditions in waterlogged soils). 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is a key enzyme in ethylene biosynthesis. Stagnant conditions hardly induced the expression of ACS1 in rcn1 roots, resulting in low ethylene production in the roots. Accumulation of saturated very-long-chain fatty acids (VLCFAs) of 24, 26, and 28 carbons was reduced in rcn1 roots. Exogenously supplied VLCFA (26 carbons) increased the expression level of ACS1 and induced aerenchyma formation in rcn1 roots. Moreover, in rice lines in which the gene encoding a fatty acid elongase, CUT1-LIKE (CUT1L; a homolog of the gene encoding Arabidopsis CUT1, which is required for cuticular wax production), was silenced, both ACS1 expression and aerenchyma formation were reduced. Interestingly, the expression of ACS1, CUT1L, and RCN1/OsABCG5 was induced predominantly in the outer part of roots under stagnant conditions. These results suggest that, in rice under oxygen-deficient conditions, VLCFAs increase ethylene production by promoting 1-aminocyclopropane-1-carboxylic acid biosynthesis in the outer part of roots, which, in turn, induces aerenchyma formation in the root cortex.Aerenchyma formation is a morphological adaptation of plants to complete submergence and waterlogging of the soil, and facilitates internal gas diffusion (Armstrong,

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The rice RCN1/OsABCG5 mutation is associated with root de-velopment in response to nutrient shortage

Cited by 6 publications

References 25 publications

Normal root elongation requires arginine produced by argininosuccinate lyase in rice

Normal root elongation requires arginine produced by argininosuccinate lyase in rice

ABCG Transporters Are Required for Suberin and Pollen Wall Extracellular Barriers inArabidopsis

Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots

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