The rice transcription factor WRKY53 suppresses herbivore-induced defenses by acting as a negative feedback modulator of map kinase activity

Hu, Lingfei; Meng, Yahan; Li, Ran; Zhang, Tongfang; Zhou, Guoxin; Wang, Qi; Lü, Jing; Lou, Yonggen

doi:10.1104/pp.15.01090

Cited by 91 publications

(141 citation statements)

References 72 publications

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“…3a). Consistently, BPH-induced transcript levels of two JA biosynthesis-related genes, a herbivore-induced 13lipoxygenase gene OsHI-LOX (Zhou et al 2009) and an allene oxide synthase (AOS) gene OsAOS1 (Hu et al 2015) were down-regulated in irSLR1 lines and up-regulated (more or less) in oeSLR1 lines compared with those in similarly treated WT plants (Fig. 3b,c).…”

Section: Osslr1 Positively Regulates Transcriptional Levels Of Two Mpsupporting

confidence: 63%

“…It has been well documented that MPKs regulate WRKYs and OsSLR1 regulates rice herbivore resistance 2155 that both MPKs and WRKYs modulate the biosynthesis of JA, JA-Ile, SA, ET and ROS by directly regulating the activity of related enzymes (Wu et al 2007;Li et al 2012). In rice, OsMPK3 and OsMPK6 are reported to modulate many WRKYs and also the JA-mediated, SA-mediated, ETmediated and H 2 O 2 -mediated pathways (Kim et al 2012;Wang et al 2013); moreover, several WRKYs, such as OsWRKY70 and OsWRKY53, influence the production of JA, ET and/or SA (Hu et al 2015;Li et al 2015). In addition, OsWRKY53 negatively regulates the activity of OsMPK3 and OsMPK6 (Hu et al 2016).…”

Section: Discussionmentioning

confidence: 99%

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Silencing OsSLR1 enhances the resistance of rice to the brown planthopper Nilaparvata lugens

Zhang

Luo

Wang

et al. 2017

Plant Cell & Environment

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DELLA proteins, negative regulators of the gibberellin (GA) pathway, play important roles in plant growth, development and pathogen resistance by regulating multiple phytohormone signals. Yet, whether and how they regulate plant herbivore resistance remain unknown. We found that the expression of the rice DELLA gene OsSLR1 was down-regulated by an infestation of female adults of the brown planthopper (BPH) Nilaparvata lugens. On one hand, OsSLR1 positively regulated BPH-induced levels of two mitogen-activated protein kinase and four WRKY transcripts, and of jasmonic acid, ethylene and H O . On the other hand, silencing OsSLR1 enhanced constitutive levels of defence-related compounds, phenolic acids, lignin and cellulose, as well as the resistance of rice to BPH in the laboratory and in the field. The increased resistance in rice with silencing of OsSLR1 is probably due to impaired JA and ethylene pathways, and, at least in part, to the increased lignin level and mechanical hardness of rice leaf sheaths. Our findings illustrate that OsSLR1, acting as an early negative regulator, plays an important role in regulating the resistance of rice to BPH by activating appropriate defence-related signalling pathways and compounds. Moreover, our data also provide new insights into relationships between plant growth and defence.

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Section: Osslr1 Positively Regulates Transcriptional Levels Of Two Mpsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Silencing OsSLR1 enhances the resistance of rice to the brown planthopper Nilaparvata lugens

Zhang

Luo

Wang

et al. 2017

Plant Cell & Environment

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“…However, it is unknown whether OsMKP1 could target other MAPKs to regulate grain size. OsMAPK3 plays a similar role as OsMAPK6 in defense responses (Kim et al ., ; Hu et al ., ). It would be interesting to investigate whether OsMKP1 could target OsMAPK3 to regulate grain size in future studies.…”

Section: Discussionmentioning

confidence: 99%

A mitogen‐activated protein kinase phosphatase influences grain size and weight in rice

Wang

et al. 2018

The Plant Journal

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Grain size and weight are directly associated with grain yield in crops. However, the molecular mechanisms that set final grain size and weight remain largely unknown. Here, we characterize two large grain mutants, large grain8-1 (large8-1) and large grain8-2 (large8-2). LARGE8 encodes the mitogen-activated protein kinase phosphatase1 (OsMKP1). Loss of function mutations in OsMKP1 results in large grains, while overexpression of OsMKP1 leads to small grains. OsMKP1 determines grain size by restricting cell proliferation in grain hulls. OsMKP1 directly interacts with and deactivates the mitogen-activated protein kinase 6 (OsMAPK6). Taken together, we identify OsMKP1 as a crucial factor that influences grain size by deactivating OsMAPK6, indicating that the reversible phosphorylation of OsMAPK6 plays important roles in determining grain size in rice.

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“…Evidence increasingly indicates that WRKYs play significant roles in plant development and in responses to biotic and abiotic stresses [5, 8, 45–47], and members of the AP2-EREBP family mediate defense against biotic and/or abiotic stress [45]. For example, it was recently found that OsWRKY70 mediates the prioritization of defense over growth by positively regulating cross-talk between JA and SA when rice is attack by C. suppressalis [47], and OsWRKY53 is a negative regulator of plant growth and an early suppressor of induced defenses [46], both of which belong to WRKY family. The function of TFs in the defense of rice against insects warrants further research.…”

Section: Discussionmentioning

confidence: 99%

Combined transcriptome and metabolome analyses to understand the dynamic responses of rice plants to attack by the rice stem borer Chilo suppressalis (Lepidoptera: Crambidae)

et al. 2016

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BackgroundRice (Oryza sativa L.), which is a staple food for more than half of the world’s population, is frequently attacked by herbivorous insects, including the rice stem borer, Chilo suppressalis. C. suppressalis substantially reduces rice yields in temperate regions of Asia, but little is known about how rice plants defend themselves against this herbivore at molecular and biochemical level.ResultsIn the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and in metabolic processes in rice plants that had been continuously fed by C. suppressalis larvae for different durations (0, 24, 48, 72, and 96 h). Furthermore, the data were validated using quantitative real-time PCR. There were 4,729 genes and 151 metabolites differently regulated when rice plants were damaged by C. suppressalis larvae. Further analyses showed that defense-related phytohormones, transcript factors, shikimate-mediated and terpenoid-related secondary metabolism were activated, whereas the growth-related counterparts were suppressed by C. suppressalis feeding. The activated defense was fueled by catabolism of energy storage compounds such as monosaccharides, which meanwhile resulted in the increased levels of metabolites that were involved in rice plant defense response. Comparable analyses showed a correspondence between transcript patterns and metabolite profiles.ConclusionThe current findings greatly enhance our understanding of the mechanisms of induced defense response in rice plants against C. suppressalis infestation at molecular and biochemical levels, and will provide clues for development of insect-resistant rice varieties.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0946-6) contains supplementary material, which is available to authorized users.

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The rice transcription factor WRKY53 suppresses herbivore-induced defenses by acting as a negative feedback modulator of map kinase activity

Cited by 91 publications

References 72 publications

Silencing OsSLR1 enhances the resistance of rice to the brown planthopper Nilaparvata lugens

Silencing OsSLR1 enhances the resistance of rice to the brown planthopper Nilaparvata lugens

A mitogen‐activated protein kinase phosphatase influences grain size and weight in rice

Combined transcriptome and metabolome analyses to understand the dynamic responses of rice plants to attack by the rice stem borer Chilo suppressalis (Lepidoptera: Crambidae)

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