The rich somatic life of <i>Wolbachia</i>

Pietri, Jose E.; DeBruhl, Heather; Sullivan, William

doi:10.1002/mbo3.390

Cited by 189 publications

(189 citation statements)

References 144 publications

(212 reference statements)

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“…Indeed, we detected DNA of several arthropod symbionts, which were probably prey‐associated, in some of the wild‐caught Badumna specimens. Among those taxa were Arsenophonus and Hamiltonella , as well as Wolbachia , which are all known to be able to survive and grow in arthropod guts after ingestion (Paula et al, ; Pietri, DeBruhl, & Sullivan, ). We also discovered DNA of the obligate endosymbionts Buchnera and Sulcia in a spider specimen that fed on spittlebugs and aphids.…”

Section: Discussionmentioning

confidence: 99%

Are you what you eat? A highly transient and prey‐influenced gut microbiome in the grey house spider Badumna longinqua

et al. 2020

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Stable core microbial communities have been described in numerous animal species and are commonly associated with fitness benefits for their hosts. Recent research, however, highlights examples of species whose microbiota are transient and environmentally derived. Here, we test the effect of diet on gut microbial community assembly in the spider Badumna longinqua. Using 16S rRNA gene amplicon sequencing combined with quantitative PCR, we analyzed diversity and abundance of the spider's gut microbes, and simultaneously characterized its prey communities using nuclear rRNA markers. We found a clear correlation between community similarity of the spider's insect prey and gut microbial DNA, suggesting that microbiome assembly is primarily diet‐driven. This assumption is supported by a feeding experiment, in which two types of prey—crickets and fruit flies—both substantially altered microbial diversity and community similarity between spiders, but did so in different ways. After cricket consumption, numerous cricket‐derived microbes appeared in the spider's gut, resulting in a rapid homogenization of microbial communities among spiders. In contrast, few prey‐associated bacteria were detected after consumption of fruit flies; instead, the microbial community was remodelled by environmentally sourced microbes, or abundance shifts of rare taxa in the spider's gut. The reshaping of the microbiota by both prey taxa mimicked a stable core microbiome in the spiders for several weeks post feeding. Our results suggest that the spider's gut microbiome undergoes pronounced temporal fluctuations, that its assembly is dictated by the consumed prey, and that different prey taxa may remodel the microbiota in drastically different ways.

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Section: Discussionmentioning

confidence: 99%

Are you what you eat? A highly transient and prey‐influenced gut microbiome in the grey house spider Badumna longinqua

et al. 2020

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“…laura (without web) and P . astrigera (without web) Endosymbionts are widely distributed in the organs and tissues of their arthropod hosts (Pietri, DeBruhl, & Sullivan, ; Sicard, Dittmer, Grève, Bouchon, & Braquart‐Varnier, ), and whether the high abundance distribution of endosymbionts in the intestinal tissues correlates with the hosts’ digestive function and immune defense is interesting. Furthermore, pathogens often use the host's gut epithelium as an entry site for systematic infections (Engel & Moran, ).…”

Section: Discussionmentioning

confidence: 99%

Taking insight into the gut microbiota of three spider species: No characteristic symbiont was found corresponding to the special feeding style of spiders

Zhang

Yao

et al. 2019

Ecology and Evolution

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Microorganisms in insect guts have been recognized as having a great impact on their hosts' nutrition, health, and behavior. Spiders are important natural enemies of pests, and the composition of the gut microbiota of spiders remains unclear. Will the bacterial taxa in spiders be same as the bacterial taxa in insects, and what are the potential functions of the gut bacteria in spiders? To gain insight into the composition of the gut bacteria in spiders and their potential function, we collected three spider species, Pardosa laura , Pardosa astrigera, and Nurscia albofasciata , in the field, and high‐throughput sequencing of the 16S rRNA V3 and V4 regions was used to investigate the diversity of gut microbiota across the three spider species. A total of 23 phyla and 150 families were identified in these three spider species. The dominant bacterial phylum across all samples was Proteobacteria. Burkholderia , Ralstonia , Ochrobactrum , Providencia , Acinetobacter , Proteus, and Rhodoplanes were the dominant genera in the guts of the three spider species. The relative abundances of Wolbachia and Rickettsiella detected in N . albofasciata were significantly higher than those in the other two spider species. The relative abundance of Thermus , Amycolatopsis , Lactococcus , Acinetobacter Microbacterium, and Koribacter detected in spider gut was different among the three spider species. Biomolecular interaction networks indicated that the microbiota in the guts had complex interactions. The results of this study also suggested that at the genus level, some of the gut bacteria taxa in the three spider species were the same as the bacteria in insect guts.

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“…Non-parametric tests were again used to compare Wolbachia prevalence between tissue types and diets. Because Wolbachia are likely to be present intracellularly in the tissues of either the host or prey and are not known to be an important functional contributor extracellularly in the gut (Pietri et al, 2016), these sequences were removed prior to downstream analysis.…”

Section: Methodsmentioning

confidence: 99%

Microbial Communities of Lycaenid Butterflies Do Not Correlate with Larval Diet

et al. 2016

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Herbivores possess many counteradaptations to plant defenses, and a growing body of research describes the role of symbiotic gut bacteria in mediating herbivorous diets among insects. However, persistent bacterial symbioses have not been found in Lepidoptera, despite the fact that perhaps 99% of the species in this order are herbivorous. We surveyed bacterial communities in the guts of larvae from 31 species of lycaenid butterflies whose caterpillars had diets ranging from obligate carnivory to strict herbivory. Contrary to our expectations, we found that the bacterial communities of carnivorous and herbivorous caterpillars do not differ in richness, diversity, or composition. Many of the observed bacterial genera are commonly found in soil and plant surfaces, and we detected known homopteran endosymbionts in the guts of homopterophagous species, suggesting that larvae acquire gut bacteria from their food and environment. These results indicate that lycaenid butterflies do not rely on specific bacterial symbioses to mediate their diverse diets, and provide further evidence of taxonomically depauperate bacterial communities among Lepidoptera.

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The rich somatic life of Wolbachia

Cited by 189 publications

References 144 publications

Are you what you eat? A highly transient and prey‐influenced gut microbiome in the grey house spider Badumna longinqua

Are you what you eat? A highly transient and prey‐influenced gut microbiome in the grey house spider Badumna longinqua

Taking insight into the gut microbiota of three spider species: No characteristic symbiont was found corresponding to the special feeding style of spiders

Microbial Communities of Lycaenid Butterflies Do Not Correlate with Larval Diet

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