The rise and fall of a toxigenic population of Microcystis aeruginosa (Cyanophyceae/Cyanobacteria)—a decade of observations in Lake Akersvatnet, Norway

Utkilen, Hans; Skulberg, Olav M.; Underdal, Bjarne; Gjølme, Nina; Skulberg, Randi; Kotai, J.

doi:10.2216/i0031-8884-35-6s-189.1

Cited by 13 publications

(7 citation statements)

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“…Similar results were reported by Park et al (1993) who suggested that increase of NO 3 -N concentration favored the growth of Microcystis at Lake Suwa in Japan. Furthermore, toxic Microcystis population collapsed when NO 3 -N concentration decreased (Utkilen et al 1996). Microcystis may not require high concentration of PO 4 -P for their growth as in our study where Microcystis bloom occurred in relatively low PO 4 -P concentration.…”

Section: Discussionsupporting

confidence: 50%

Influence of environmental parameters on toxic cyanobacterial bloom occurrence in a Lake of Bangladesh

Affan

Touliabah

Al-Harbi

et al. 2016

Rend. Fis. Acc. Lincei

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Increased nutrients have led cyanobacteria to become dominant in many ponds, lakes and reservoirs in many countries of the world. The occurrence and abundance of cyanobacterial population were monitored in a lake (known as Ishakha Lake) at Bangladesh Agricultural University campus, Mymensingh, Bangladesh. The hydrographic parameters such as water temperature, pH, chlorophyll a and nutrients (NO 3 -N and PO 4 -P) were recorded to find out their relationship with the cyanobacterial bloom formation. During the study period five species of cyanobacteria namely, Microcystis aeruginosa Kütz., M. wesenbergii Kom., M. botrys Teli., M. viridis (A. Br.) Lemm. and Anabaena circinalis Rabenh., were identified and among them M. aeruginosa was the dominant species during the bloom period. At the peak period of bloom, the highest cell density of M. aeruginosa was 1550 9 10 3 cells ml -1 which comprised 97.45 % among the blue-green algae and 96.84 % to the total phytoplankton. The initiation and persistence of natural bloom of cyanobacteria, especially Microcystis spp. was found to be controlled by relatively high temperature ([25.00°C) and nutrients, especially high NO 3 -N (3.80 mg l -1 ) concentration. Temperature and NO 3 -N showed positive correlation with cyanobacterial cells abundance which were r = 0.62 and r = 0.92. Therefore, it could be said that temperature and NO 3 -N made a favorable circumstance to form cyanobacterial bloom in as Ishakha Lake. concentration of MCs 37,460.00 pg ml -1 at the peak period of bloom.

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Section: Discussionsupporting

confidence: 50%

Influence of environmental parameters on toxic cyanobacterial bloom occurrence in a Lake of Bangladesh

Affan

Touliabah

Al-Harbi

et al. 2016

Rend. Fis. Acc. Lincei

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“…Lowering of the water level due to canalization during the year 1968 led to a shift in species composition. By the year 1994, the phytoplankton community was dominated by species of Anabaena and Aphanizomenon and the dinoflagellate Ceratium hirundinella [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization of bacterial community associated with phytoplankton bloom in a eutrophic lake in South Norway using 16S rRNA gene amplicon sequence analysis

et al. 2017

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Interactions between different phytoplankton taxa and heterotrophic bacterial communities within aquatic environments can differentially support growth of various heterotrophic bacterial species. In this study, phytoplankton diversity was studied using traditional microscopic techniques and the bacterial communities associated with phytoplankton bloom were studied using High Throughput Sequencing (HTS) analysis of 16S rRNA gene amplicons from the V1-V3 and V3-V4 hypervariable regions. Samples were collected from Lake Akersvannet, a eutrophic lake in South Norway, during the growth season from June to August 2013. Microscopic examination revealed that the phytoplankton community was mostly represented by Cyanobacteria and the dinoflagellate Ceratium hirundinella. The HTS results revealed that Proteobacteria (Alpha, Beta, and Gamma), Bacteriodetes, Cyanobacteria, Actinobacteria and Verrucomicrobia dominated the bacterial community, with varying relative abundances throughout the sampling season. Species level identification of Cyanobacteria showed a mixed population of Aphanizomenon flos-aquae, Microcystis aeruginosa and Woronichinia naegeliana. A significant proportion of the microbial community was composed of unclassified taxa which might represent locally adapted freshwater bacterial groups. Comparison of cyanobacterial species composition from HTS and microscopy revealed quantitative discrepancies, indicating a need for cross validation of results. To our knowledge, this is the first study that uses HTS methods for studying the bacterial community associated with phytoplankton blooms in a Norwegian lake. The study demonstrates the value of considering results from multiple methods when studying bacterial communities.

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“…aeruginosa is a unicellular, colonial freshwater cyanobacterium which often forms blooms during warmer months in eutrophic lakes and reservoirs (37). For this reason, much research has been concerned with the environmental factors which lead to bloom formation and toxin production in this species.…”

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confidence: 99%

Cellular Microcystin Content in N-Limited Microcystis aeruginosa Can Be Predicted from Growth Rate

Long

Jones

Orr

2001

Appl Environ Microbiol

276

194

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Cell quotas of microcystin (Q MCYST ; femtomoles of MCYST per cell), protein, and chlorophyll a (Chl a), cell dry weight, and cell volume were measured over a range of growth rates in N-limited chemostat cultures of the toxic cyanobacterium Microcystis aeruginosa MASH 01-A19. There was a positive linear relationship between Q MCYST and specific growth rate (), from which we propose a generalized model that enables Q MCYST at any nutrient-limited growth rate to be predicted based on a single batch culture experiment. The model predicts Q MCYST from , max (maximum specific growth rate), Q MCYSTmax (maximum cell quota), and Q MCYSTmin (minimum cell quota). Under the conditions examined in this study, we predict a Q MCYSTmax of 0.129 fmol cell ؊1 at max and a Q MCYSTmin of 0.050 fmol cell ؊1 at ‫؍‬ 0. Net MCYST production rate (R MCYST ) asymptotes to zero at ‫؍‬ 0 and reaches a maximum of 0.155 fmol cell The microcystins (MCYSTs) are a group of cyclic heptapeptide toxins produced by several cyanobacterial species. Of the more than 60 MCYSTs characterized to date (19,27,29), most are potent inhibitors of protein phosphatases 1 and 2A from both plants and animals (17). One of the most common MCYST-producing cyanobacteria is the bloom-forming Microcystis aeruginosa (Kützing) Lemmermann. Due to the widespread distribution and potential toxicity of this species (toxic strains have been found worldwide), M. aeruginosa has been implicated in a number of animal-poisoning incidents (e.g., reference 7) and more recently in human fatalities (11,23).M. aeruginosa is a unicellular, colonial freshwater cyanobacterium which often forms blooms during warmer months in eutrophic lakes and reservoirs (37). For this reason, much research has been concerned with the environmental factors which lead to bloom formation and toxin production in this species. A wide range of batch culture studies have shown that the variables influencing MCYST content include trace metal supply (15), nitrogen (N) and phosphorus (P) (31), light and temperature (38), and pH (34). Comparative studies on MCYST production by M. aeruginosa in continuous culture, however, have been limited to examination of the effects of photon irradiance (35), N, P, and Fe 3ϩ limitation (16, 36), and more recently P limitation (20). Despite this considerable pool of data concerning MCYST production, few studies (with the exception of the work carried out by Rapala and coworkers [25,26]) have been able to quantitatively relate MCYST content to any growth determinant.In a previous batch culture study, we presented data on the effect of N supply on the cellular production of MCYSTs (21). This work showed that the net specific rate of MCYST production was equal to the cell specific growth rate. The application of these findings to previously published batch culture studies suggested that the relationship held under a variety of culture conditions and that MCYST production was indirectly affected by environmental factors through their effects on cell division. A consequence of this l...

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The rise and fall of a toxigenic population of Microcystis aeruginosa (Cyanophyceae/Cyanobacteria)—a decade of observations in Lake Akersvatnet, Norway

Cited by 13 publications

References 0 publications

Influence of environmental parameters on toxic cyanobacterial bloom occurrence in a Lake of Bangladesh

Influence of environmental parameters on toxic cyanobacterial bloom occurrence in a Lake of Bangladesh

Characterization of bacterial community associated with phytoplankton bloom in a eutrophic lake in South Norway using 16S rRNA gene amplicon sequence analysis

Cellular Microcystin Content in N-Limited Microcystis aeruginosa Can Be Predicted from Growth Rate

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