The RNA-binding protein xCIRP2 is involved in apoptotic tail regression during metamorphosis in Xenopus laevis tadpoles

Eto, Kojiro; Iwama, T.; Tajima, Tatsuya; Abe, Shinichi

doi:10.1016/j.ygcen.2012.07.017

Cited by 3 publications

(3 citation statements)

References 35 publications

(89 reference statements)

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“…Namely, we discovered for the first time the mild hypothermia‐responded signaling pathway comprising NF‐κB p65 and RBM3 that contributes to sustaining cell survival. In accordance with our current study on the prosurvival/antiapoptotic function of RBM3, our previous study demonstrates that newt and frog homologs of human RBM3 may have inhibitory effect against apoptosis induced in spermatogonia during spermatogenesis and in tadpole tails during metamorphosis, respectively . This suggested that the glycine‐rich RNA‐binding protein family members, including RBM3 and its newt and frog homologs, exert the prosurvival function.…”

Section: Discussionmentioning

confidence: 99%

“…In accordance with our current study on the prosurvival/ antiapoptotic function of RBM3, our previous study demonstrates that newt and frog homologs of human RBM3 may have inhibitory effect against apoptosis induced in spermatogonia during spermatogenesis and in tadpole tails during metamorphosis, respectively. 18,57 This suggested that the glycine-rich RNA-binding protein family members, including RBM3 and its newt and frog homologs, exert the prosurvival function. RBM3 is known to maintain the secondary structure of target mRNA molecules correctly, block their degradation, and promote their translation.…”

Section: The Nf-κb P65-rbm3 Signaling Axis Is Required For Blockingmentioning

confidence: 99%

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RBM3 expression is upregulated by NF‐κB p65 activity, protecting cells from apoptosis, during mild hypothermia

Ushio

Eto²

2018

J of Cellular Biochemistry

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The RNA-binding protein RBM3, a cold shock protein whose expression is elevated under hypothermic conditions, plays an important role in cell survival; however, little is known about the mechanism underlying the mild hypothermia-mediated regulation of RBM3 expression and apoptosis. Here we show that the transcription factor NF-κB p65 is phosphorylated at Ser276 and activates RBM3 gene transcription via binding to a particular element within the promoter region in response to induced hypothermia, elevating the protein expression, and suppressing apoptosis. Treatment with caffeic acid phenethyl ester (CAPE), a potent and specific inhibitor that suppresses the translocation of NF-κB p65 from the cytoplasm to the nucleus, resulted in decreased levels of RBM3 mRNA and protein and increased incidence of apoptosis despite cells were cultured under hypothermic conditions. Overexpression of RBM3 abolished the induction of apoptosis in cells treated with CAPE, indicating that NF-κB p65-upregulated RBM3 expression is necessary for the suppression of apoptosis. In addition, experiments with cells overexpressing RBM3 supported the finding demonstrating that the mild hypothermia-mediated higher expression of RBM3 suppressed the induction of apoptosis. Conversely, experiments with cells deficient in RBM3 supported the finding demonstrating that the CAPE-mediated loss of RBM3 induced apoptosis. These results suggest that NF-κB p65 is a critical mediator of mild hypothermia, to which cells are exposed as an extracellular environment, and a central inducer of RBM3 expression, which is responsible for preventing cells from apoptosis. Moreover, CAPE may have a potential for the application to a therapeutic agent for the treatment of cancers.

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Section: Discussionmentioning

confidence: 99%

Section: The Nf-κb P65-rbm3 Signaling Axis Is Required For Blockingmentioning

confidence: 99%

RBM3 expression is upregulated by NF‐κB p65 activity, protecting cells from apoptosis, during mild hypothermia

Ushio

Eto²

2018

J of Cellular Biochemistry

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“…By comparing tissue-specific differences in the responsiveness to TH, we suggest that liver and intestine tissues are more suitable than tail and brain tissues as test materials for in vivo assay of TH signaling disrupting effects. Certainly, tail tissue can be used in in vitro study due to its advantage of easy culture in vitro (Eto et al, 2012;Tseng et al, 2007). During R. nigromaculata development, the intestine undergoes a dramatic morphological change, but the liver hardly changes in morphology.…”

Section: Responsiveness Of Tr Expression To Exogenous Tr Agonist and mentioning

confidence: 99%

Molecular characterization and developmental expression patterns of thyroid hormone receptors (TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata

Lou

Zhang

Ren

et al. 2014

Journal of Environmental Sciences

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Considering some advantages of Rana nigromaculata as an experimental species, we propose that this species, like Xenopus laevis, could be used to assay thyroid hormone (TH) signaling disrupting actions. To validate the utilizability of R. nigromaculata, we investigated the responsiveness of R. nigromaculata to a TH receptor (TR) agonist (T3) and antagonist (amiodarone) by analyzing expression, based on characterizing TR cDNA and developmental expression patterns. With high levels of identity with the corresponding genes in X. laevis, both TRα and TRβ in R. nigromaculata exhibited roughly similar developmental expression patterns to those of X. laevis, in spite of some species-specific differences. Both TRα and TRβ expression had greater changes in the liver and intestine than in the tail and brain during metamorphosis. T3 exposure for 2 days induced more dramatic increases of TRβ expression in stage 27 than in stage 34 tadpoles but not in stage 42 tadpoles, showing that the responsiveness of R. nigromaculata to TH decreased with development and disappeared at the onset of metamorphic climax.Corresponding to greater changes of TRβ expression in the liver and intestine than in the tail and brain during metamorphosis, the liver and intestine had higher responsiveness to exogenous T3 than the tail and brain. Amiodarone inhibited T3-induced TRβ expression. Our results show that R. nigromaculata can be used as a model species for assaying TH signaling disrupting actions by analyzing TRβ expression, and intestine tissues at stage 27 are ideal test materials due to high responsiveness and easy accessibility.

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Comparison of thyroid hormone-dependent gene responses in vivo and in organ culture of the American bullfrog ( Rana (Lithobates) catesbeiana ) lung

Veldhoen

Stevenson

Helbing

2015

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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The RNA-binding protein xCIRP2 is involved in apoptotic tail regression during metamorphosis in Xenopus laevis tadpoles

Cited by 3 publications

References 35 publications

RBM3 expression is upregulated by NF‐κB p65 activity, protecting cells from apoptosis, during mild hypothermia

RBM3 expression is upregulated by NF‐κB p65 activity, protecting cells from apoptosis, during mild hypothermia

Molecular characterization and developmental expression patterns of thyroid hormone receptors (TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata

Comparison of thyroid hormone-dependent gene responses in vivo and in organ culture of the American bullfrog ( Rana (Lithobates) catesbeiana ) lung

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