The RNA profile of porcine parvovirus 4, a boca-like virus, is unique among the parvoviruses

Cheung, Andrew; Long, Jinxue; Huang, Lv; Yuan, Sheng

doi:10.1007/s00705-011-1072-2

Cited by 6 publications

(5 citation statements)

References 19 publications

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“…This virus was tentatively named PPV4. However, its genome structure and RNA profile showed it had the characteristics of bocaviruses, herein, so it was renamed PBoV2 [23,30]. Considering the major differences within the PBoV2 genome, it should include two genotypes.…”

Section: Discussion and Nomenclature Of Pbovsmentioning

confidence: 99%

Sequence Comparison and Phylogenetic Analysis of Several Emerging Porcine Bocaviruses and a Proposal Regarding Nomenclature

Li¹

2015

Virol Mycol

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The first Bocavirus (bovine parvovirus 1, BPV1) was discovered in calves with diarrhea in 1961. Since then, a series of bocaviruses have emerged in many animals including dogs, humans, swine, gorillas, California sea lions, and cats. These viruses are associated with digestive tract and respiratory tract diseases. Several emerging porcine bocaviruses (PBoVs) have been reported on pig farms in the past four years. However, the nomenclature used to describe these viruses has been confused and irregular. The goal of this study was to compare the sequences of these emerging PBoVs, perform phylogenetic analysis, and present a proposal regarding their nomenclature. Genome sequences of some PBoVs and other bocaviruses were downloaded from the GenBank database. Sequence similarity, sequence mutation, deletion, or insertion, and sequence recombination among reference bocaviruses were obtained using DNAStar software, and its genetic evolution was determined using MEGA 5.1 software. According to the differences in their sequences and genome structure, the nomenclature of PBoVs was proposed and they were divided into four different species (from PBoV1 to PBoV4) and many different genotypes. Among them, both PBoV1 and PBoV2 had two genotypes, PBoV1-a and PBoV1-b and PBoV2-a and PBoV2-b, respectively. PBoV3 included three genotypes, PBoV3-a, PBoV3-b and PBoV3-c, respectively. In addition, for PBoV4, there were at least eight genotypes (PBoV4-a to PBoV4-h) in intraspecies. In conclusion, PBoVs were highly genetically diversified. The proposed nomenclature of PBoVs was used in the present studies, it could help us to better understand and standardize the nomenclature of PBoVs.

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Section: Discussion and Nomenclature Of Pbovsmentioning

confidence: 99%

Sequence Comparison and Phylogenetic Analysis of Several Emerging Porcine Bocaviruses and a Proposal Regarding Nomenclature

Li¹

2015

Virol Mycol

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“…Two major putative ORFs (ORF1 and ORF2) were predicted, indicating a genome organization similar to PPV1, PPV2, and PPV3, but different from PPV4, which contains an additional ORF3 similar to bocavirus (3, 7). The predicted ORF1 (bp 862 to 2667) and ORF2 (bp 2787 to 5762) encode proteins of 601 and 991 amino acids, which are 3 and 263 amino acids longer than ORF1 (598 amino acids) and ORF2 (728 amino acids) of PPV4, respectively.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of a Novel Porcine Parvovirus (PPV) Provisionally Designated PPV5

2013

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“…It has been shown in clinical cases to be a commonly mixed infection with Pseudorabies virus (PRV), classical swine fever virus (CSFV), Porcine Reproductive and Respiratory Syndrome virus (PRRSV). At present, seven genotypes of PPV have been found in the world, which have some differences in genomes and pathogenicity, and isolated and identified in 2017 [5][6][7][8][9][10]. PPV1 is the most common type in pigs, which has a higher positive detection rate in Chinese pig herds and is harmful to the pig industry.…”

Section: Introductionmentioning

confidence: 99%

Two novel protein chips for the detection of antibodies against porcine parvovirus

Hou

et al. 2020

BMC Vet Res

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Background: PPV is one of the most important pathogens causing porcine reproductive disorder. It has been shown in clinical cases to be a commonly mixed infection with other important swine diseases which can aggravate the severity of the disease and bring serious economic losses to the pig industry. Serological methods, such as hemagglutination inhibition assays (HAI), serum neutralization (SN), and the modified direct complementfixation (MDCF) test were utilized earlier, whereas the enzyme-linked immunosorbent assay (ELISA) is the most frequently applied assay to detect PPV-specific antibodies. Results: We establish the visible protein chip and the cyanine dye 3 (Cy3)-labeled protein chip to detect the clinical serum from pigs. In this study, the recombinant protein VP2 of PPV was expressed in E.coli, purified with nickel magnetic beads, and then printed onto epoxy-coated glass slides for preparation of the protein chip. After a series of experiments, the conditions of antigen protein concentration, incubation time of primary antibody or secondary antibody, and optimal serum dilution fold were optimized, resulting in a successful visible protein chip and Cy3labeled protein chip. The results showed that the positive serum, diluted up to 6000-fold, can be detected by the visible protein chip, and the positive serum, diluted up to 12,800-fold, can be detected by the Cy3-labeled protein chip, suggesting the high sensitivity of these protein chips. Moreover, the positive detection ratio, sensitivity, and specificity of these two kinds of protein chips were higher than those of commercial ELISA antibody detection kits. Conclusion: Overall, these two protein chips can be used to rapidly diagnose clinical samples with high throughput.

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The RNA profile of porcine parvovirus 4, a boca-like virus, is unique among the parvoviruses

Cited by 6 publications

References 19 publications

Sequence Comparison and Phylogenetic Analysis of Several Emerging Porcine Bocaviruses and a Proposal Regarding Nomenclature

Sequence Comparison and Phylogenetic Analysis of Several Emerging Porcine Bocaviruses and a Proposal Regarding Nomenclature

Complete Genome Sequence of a Novel Porcine Parvovirus (PPV) Provisionally Designated PPV5

Two novel protein chips for the detection of antibodies against porcine parvovirus

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