The Role and Molecular Mechanism of Non-Coding RNAs in Pathological Cardiac Remodeling

Gao, Jinling; Xu, Wu; Wang, Jianxun; Wang, Kun; Li, Peifeng

doi:10.3390/ijms18030608

Cited by 54 publications

(42 citation statements)

References 95 publications

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“…Non-coding RNAs (ncRNAs), which include miRNAs, long ncRNAs and circRNAs, are involved in the development of several diseases including pathological cardiac remodeling, nervous system diseases and several cancers (16)(17)(18)(19). circRNAs are a novel type of ncRNA characterized by a stable structure and highly specific tissue expression.…”

Section: Discussionmentioning

confidence: 99%

circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis

Dou

et al. 2019

Mol Med Report

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Chondrocyte extracellular matrix-related circular RNAs (circRNA-CER) have been demonstrated to be involved in various diseases. However, its role in the development of human breast cancer is not clearly understood. The aims of the present study were to assess circRNA-CER expression in paired cancer tissue and adjacent non-tumor tissue from 24 patients with breast cancer, and to explore the roles and mechanisms by which circRNA-CER mediates the malignant progression of breast cancer cells. The results revealed that circRNA-CER and matrix metalloproteinase 13 (MMP13) were upregulated, whereas miR-136 was downregulated in breast cancer tissues compared with adjacent non-tumor tissues. In vitro silencing of circRNA-CER using small interfering RNA (siRNA) had inhibitory effects on MCF-7 breast cancer cell proliferation and migration, and similar results were obtained following overexpression of microRNA (miR)-136 in MCF-7 cells by transfection with miR-136 mimics. The subsequent mechanistic study revealed that the expression levels of MMP13 were significantly lower in MCF-7 cells following transfection with miR-136 mimics, and silencing of circRNA-CER enhanced miR-136 and decreased MMP13 expression levels. Furthermore, silencing of miR-136 by transfection with miR-136 inhibitors resulted in an increase in MCF-7 cell proliferation and migration. miR-136 inhibitor-derived biological effects were reversed by co-transfection of cells with miR-136 inhibitors and circRNA-CER siRNA. Taken together, the present results suggested that circRNA-CER may serve an important role in the progression of breast cancer by regulating the activity of the miR-136/MMP13 axis, and may be a potential biomarker for the prediction and treatment of breast cancer.

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Section: Discussionmentioning

confidence: 99%

circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis

Dou

et al. 2019

Mol Med Report

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“…The pathogenic role of miRNA was first reported in chronic lymphocytic leukemia where the miRNA cluster, miR-15a/16–1, is frequently lost, thereby acting as a tumor suppressor [ 58 ]. Later, many transcriptome studies have shown that miRNA are aberrantly expressed both in various human cancers and other diseases like cardiological and neurological ones [ 56 , 59 , 60 ]. Moreover, since miRNA expression profiling is able to differentiate between normal and pathological states they could be used as novel biomarkers in the diagnosis and prognosis of several human diseases.…”

Section: Novel Insights From Transcriptome Studies By Next Generatmentioning

confidence: 99%

“…Besides, since many lncRNAs are antisense transcripts, the construction of directional libraries has allowed their better detection and discrimination from mRNA; so far, many different methods have been developed to preserve the orientation of the original RNA in the final sequencing library, and to facilitate strand-specific analysis of the resulting data [ 67 , 68 ]. As for the other ncRNA, even the deregulation of numerous lncRNA has been observed in many human diseases, from cancer to cardiac pathologies or neurodegenerative disorders [ 59 , 60 , 64 ]. For instance, among the others, the well-known MALAT1 , one of the first lncRNAs to be associated with a human disease, was initially described as metastasis associated in lung adenocarcinoma transcript; then further studies demonstrated that it is involved also in other disorders such as diabetes complications, since its expression was found to be augmented in ischemic limbs [ 60 ].…”

Section: Novel Insights From Transcriptome Studies By Next Generatmentioning

confidence: 99%

Transcriptome Profiling in Human Diseases: New Advances and Perspectives

Casamassimi

Federico

Rienzo

et al. 2017

IJMS

223

153

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In the last decades, transcriptome profiling has been one of the most utilized approaches to investigate human diseases at the molecular level. Through expression studies, many molecular biomarkers and therapeutic targets have been found for several human pathologies. This number is continuously increasing thanks to total RNA sequencing. Indeed, this new technology has completely revolutionized transcriptome analysis allowing the quantification of gene expression levels and allele-specific expression in a single experiment, as well as to identify novel genes, splice isoforms, fusion transcripts, and to investigate the world of non-coding RNA at an unprecedented level. RNA sequencing has also been employed in important projects, like ENCODE (Encyclopedia of the regulatory elements) and TCGA (The Cancer Genome Atlas), to provide a snapshot of the transcriptome of dozens of cell lines and thousands of primary tumor specimens. Moreover, these studies have also paved the way to the development of data integration approaches in order to facilitate management and analysis of data and to identify novel disease markers and molecular targets to use in the clinics. In this scenario, several ongoing clinical trials utilize transcriptome profiling through RNA sequencing strategies as an important instrument in the diagnosis of numerous human pathologies.

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“…28 Our results, demonstrating associations of the lncRNAs MHRT, lncRNAs are involved in the regulation of cardiac remodelling via several different mechanisms, suggesting they represent potential therapeutic targets for the treatment of cardiac hypertrophy, heart failure and other cardiac disorders. 9,16,29,30 The manipulation of cardiac-related lncRNAs represents a new avenue for controlling the gene regulatory networks that are involved in cardiac diseases. MHRT, FENDRR and CARMEN could potentially represent attractive targets for future therapeutic strategies.…”

Section: Sion Inhibition Of Pathological Lvh Is An Attractive Therapmentioning

confidence: 99%

The long non‐coding RNAs MHRT,FENDRR and CARMEN, their expression levels in peripheral blood mononuclear cells in patients with essential hypertension and their relation to heart hypertrophy

Kontaraki

Marketou

Kochiadakis

et al. 2018

Clin Exp Pharma Physio

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Long non-coding RNAs (lncRNAs) participate in the modulation of cardiac hypertrophy, and they represent potential therapeutic targets in cardiovascular disease. We investigated the expression profiles of selected lncRNAs in peripheral blood mononuclear cells of patients with essential hypertension in relation to left ventricular hypertrophy. We assessed the expression levels of the lncRNAs MHRT, FENDRR and CARMEN using real-time reverse transcription polymerase chain reaction. Hypertensive patients showed significantly higher MHRT, FENDRR and CARMEN expression levels compared with healthy controls. In addition, we observed significant negative correlations of MHRT (r = -0.323, P = 0.003) and FENDRR (r = -0.380, P = 0.001) and a positive correlation of CARMEN (r = 0.458, P < 0.001) expression levels with left ventricular mass index. Our data reveal that the lncRNAs MHRT, FENDRR and CARMEN show distinct expression profiles in hypertensive patients and they possibly represent candidate therapeutic targets in hypertensive heart disease.

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The Role and Molecular Mechanism of Non-Coding RNAs in Pathological Cardiac Remodeling

Cited by 54 publications

References 95 publications

circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis

circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis

Transcriptome Profiling in Human Diseases: New Advances and Perspectives

The long non‐coding RNAs MHRT,FENDRR and CARMEN, their expression levels in peripheral blood mononuclear cells in patients with essential hypertension and their relation to heart hypertrophy

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