The role of activated T lymphocytes in gastrointestinal disease

MacDonald, T T

doi:10.1111/j.1365-2222.1990.tb02679.x

Cited by 57 publications

(20 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the reactivation stage, Th1 and Th17 exhibited similar expression levels of the activation markers CD25 and CD69 (22,23) as well as the chemokine receptor CCR7 (24). Remarkably, however, reactivated Th1 and Th17 showed significantly different expression levels for all other tested markers.…”

Section: Cultured Th1 and Th17 Differ In Their Expression Of Surface mentioning

confidence: 91%

Both Th1 and Th17 Are Immunopathogenic but Differ in Other Key Biological Activities

Cox

Shi²,

Yin³

et al. 2008

The Journal of Immunology

View full text Add to dashboard Cite

The role of Th17 lymphocytes in immunopathogenic processes has been well established, but little is known about their basic cell features. In this study, we compared polarized Th1 and Th17 for key biological activities related to pathogenicity and trafficking. Th1 and Th17 lineages were derived from TCR-transgenic CD4 murine cells specific against hen egg lysozyme. When adoptively transferred into mice expressing hen egg lysozyme in their eyes, both Th1 and Th17 induced ocular inflammation but with slight differences in histological pathology. PCR analysis revealed selective expression of IFN-γ or IL-17 in eyes of Th1 or Th17 recipients, respectively. Additionally, Th1 and Th17 were found to differ in three other key activities: 1) Th17 cells were inferior to Th1 cells in their capacity to trigger massive lymphoid expansion and splenomegaly; 2) the proportion of Th1 cells among infiltrating cells in inflamed recipient eyes declined rapidly, becoming a minority by day 7, whereas Th17 cells remained in the majority throughout this period; and 3) remarkable differences were noted between Th1 and Th17 cells in their expression of certain surface markers. In particular, reactivated Th1 expressed higher levels of CD49d and α4β7 (mucosal homing) in vitro and higher levels of CXCR3 (Th1 trafficking) in vivo. Reactivated Th17, however, expressed higher levels of αEβ7 (epithelial tissue homing) and CD38 (activation, maturation and trafficking) in vitro, but in vivo Th17 expressed higher levels of α4β7 and CCR6 (lymphocyte trafficking). These data reveal that Th1 and Th17 cells differ in several key biological activities influencing migration and pathogenic behavior during inflammatory disease.

show abstract

Section: Cultured Th1 and Th17 Differ In Their Expression Of Surface mentioning

confidence: 91%

Both Th1 and Th17 Are Immunopathogenic but Differ in Other Key Biological Activities

Cox

Shi²,

Yin³

et al. 2008

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…To determine the expression of function-related surface markers on host CD4 ϩ lymphocytes in recipient mice, we conducted FCM analysis with Abs against two activation markers, CD25 and CD69 (19,20), and three molecules that determine to a large extent the migratory properties of T lymphocytes, i.e., the chemokine receptor CXCR3 (8 -10) and the adhesion molecules CD49d (VLA-4) (8,11,12) and CD62L (L-selectin) (8,13,14). Fig.…”

Section: Phenotype Changes On Host Cd4mentioning

confidence: 99%

Active Participation of Antigen-Nonspecific Lymphoid Cells in Immune-Mediated Inflammation

Chen¹,

Fujimoto²,

Vistica³

et al. 2006

The Journal of Immunology

View full text Add to dashboard Cite

The pathogenic process of tissue-specific autoimmune disease depends to a large extent on recruitment of Ag-nonspecific cells into the target tissue. Little is known, however, about the recruitment process and the features that characterize the recruited cells. In this study, we analyzed the recruitment of Ag-nonspecific lymphoid cells into an inflammatory site by using an experimental system in which TCR-transgenic Th1 cells are adoptively transferred to induce ocular inflammation in recipient mice that express the target Ag in their eyes. A sharp increase in number of all host cell populations was observed in the recipient spleen, reaching a peak on day 4 postcell transfer and declining thereafter. A large portion of the host’s spleen CD4 cells underwent phenotypic changes that facilitate their migration into the target organ, the eye. These changes included increased expression of the chemokine receptor CXCR3, and the adhesion molecule CD49d, as well as a decline in expression of CD62L. The host lymphocytes migrated into the recipient mouse eye more slowly than the donor cells, but became the great majority of the infiltrating cells at the peak of inflammation on day 7 postcell injection. Interestingly, the mass migration of host T cells was preceded by an influx of host dendritic cells, that reached their peak on day 4 postcell injection. The eye-infiltrating host CD4 lymphocytes underwent additional changes, acquiring a profile of activated lymphocytes, i.e., up-regulation of CD25 and CD69. Our results thus provide new information about the active participation of Ag-nonspecific lymphoid cells in immune-mediated inflammation.

show abstract

“…54 T cell mediated hypersensitivity and plasma cells also play an important role in tissue damage in CD. 55 Activation of lymphocytes can, however, not reliably be identified on H&E stained sections. This can be done by immunohistochemistry, which allows the study of the expression of activation markers such as lymphocyte function associated antigen (LFA) on immune competent cells, of adhesion molecules such as the intercellular adhesion molecule (ICAM-1) on endothelial or other cells, or the expression of markers on other cells, induced by activated lymphocytes.…”

Section: Microscopic Disease Activity In Ibdmentioning

confidence: 99%