The Role of Antibiotics in<i>Campylobacter pylori</i>Associated Peptic Ulcer Disease

Bayerdörffer, Ekkehard; Ottenjann, R

doi:10.3109/00365528809091721

Cited by 86 publications

(27 citation statements)

References 25 publications

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“…Compound (9) showed the highest inhibitory potential followed by compounds 9 = 14 = 10 > 8 > 13 > 12 > 11 > 3 > (6 = 7) ³ 1 ³ 4 > 2 > 5. These observations suggest that compounds (8)- (14) are strong inhibitors; compounds (1), (6) and (7) are moderate urease inhibitors while compounds (2), (4) and (5) are weak inhibitors of the urease enzyme. Compounds (8), (9), (10), (12) and (14) has comparable or better inhibition at 500 µM concentration and IC 50 values to the standard drug thiourea, whereas 11 and 13 though showed comparable inhibition at 500 µM concentration but there higher IC 50 values make them less likely contenders in comparison to standard drug.…”

Section: Biological Activity: Urease Inhibition (In Vitro)mentioning

confidence: 89%

“…3 On the other hand in medical and veterinary interest, urease is a virulence factor in certain human and animal pathogens; it participates in the development of kidney stones, pyelonephritis, peptic ulcers, and other disease states. 4 The obvious remedy for treating bacterial infection with antimicrobials has often proven futile, 5 and only a few combination regiments have reached clinical practices. Thus the need for alternative or novel treatment is evident.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and Urease Inhibition Studies of Barbituric and Thiobarbituric Acid Derived Sulphonamides

Rauf

ahmed

Qureshi

et al. 2011

J Chinese Chemical Soc

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Various novel barbituric and thiobarbituric acid derived sulphonamides were synthesized in excellent yield via three components single pot reaction; and these were screened for in vitro urease inhibition studies against jack bean urease. The compounds 1‐7 were found to exhibit a low to moderate activity whereas compounds 8‐14 showed a significant activity (88.3‐99.9% inhibition determined at 500 μM concentration). Structures of the synthesized compounds were confirmed by 1H‐NMR, 13C‐NMR, mass spectrometry and elemental analysis data.

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Section: Biological Activity: Urease Inhibition (In Vitro)mentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Synthesis and Urease Inhibition Studies of Barbituric and Thiobarbituric Acid Derived Sulphonamides

Rauf

ahmed

Qureshi

et al. 2011

J Chinese Chemical Soc

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“…Reaction mixtures comprising 25 μL of enzyme (jack bean urease) solution and 55 μL of buffers containing 100 mM urea were incubated with 5 μL of test compounds 13 C NMR (100 MHz) data of compound 1-3 in MeOD.…”

Section: Urease Assay and Inhibitionmentioning

confidence: 99%

“…However, in medical and veterinary interest, urease is a virulence factor in certain human and animal pathogens; it participates in the development of kidney stones, pyelonephritis, peptic ulcers, and other disease states 12 . The obvious remedy for treating bacterial infection with antimicrobials has often proven futile 13 , and only a few combination regiments have reached clinical practice. Thus the need for alternative or novel treatment is evident.…”

Section: Introductionmentioning

confidence: 99%

Urease inhibitors from Indigofera gerardiana Wall.

Tariq

Ahmad

Obaidullah

et al. 2010

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…We adapted the PFGE analysis for H. pylori by using EcoRI and slightly modified our laboratory methods to improve the typing of isolates (typeability was 97%).Helicobacter pylori is associated with upper gastrointestinal diseases (1,3,4,5). Pulsed-field gel electrophoresis (PFGE) is usually used for typing bacteria for epidemiologic purposes.…”

mentioning

confidence: 99%

Use of the Restriction Enzyme EcoRI for Pulsed-Field Gel Electrophoretic Analysis of Helicobacter pylori

Hosaka

Irinoda²,

Nakano³

et al. 2005

J Clin Microbiol

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Pulsed-field gel electrophoretic (PFGE) analysis of Helicobacter pylori isolates is not commonly employed because of the inability to compare the typing with other typing systems. We adapted the PFGE analysis for H. pylori by using EcoRI and slightly modified our laboratory methods to improve the typing of isolates (typeability was 97%).Helicobacter pylori is associated with upper gastrointestinal diseases (1,3,4,5). Pulsed-field gel electrophoresis (PFGE) is usually used for typing bacteria for epidemiologic purposes. However, PFGE analysis of H. pylori isolates (2,6,7,8) is not commonly used, because of the inability to compare the typing with other typing systems (2). Our laboratory previously investigated using PFGE analysis for clinically isolated bacteria (9). However, this method was not effective for the analysis of H. pylori strains. To improve typing, we selected the restriction enzyme EcoRI to cut the chromosomal DNA and also modified our laboratory methods.A total of 71 isolates of H. pylori were collected, which were obtained from 31 patients. If H. pylori was isolated, one to four individual colonies were cultured for 4 to 7 days on agar plates for isolation (Eiken Chemical, Tokyo, Japan) in a gas mixture of 85% N 2 -5% O 2 -10% CO 2 at 35°C. Colonies were purified by passage on brucella agar plates (containing 10% horse serum), harvested from the plate by scraping, and suspended in 10% glycerol for storage at Ϫ80°C.We performed PFGE analysis as follows. H. pylori strains harvested from plates by scraping were suspended in 100 ml of brucella broth (Difco, Detroit, Mich.) supplemented with 10% horse serum (preheated to 56°C for 30 min) and cultured with shaking for 4 to 7 days at 35°C in a gas mixture (85% N 2 -5% O 2 -10% CO 2 ). The organisms were harvested by centrifugation, washed in a saline-EDTA solution (0.15 M NaCl, 10 mM EDTA, pH 8.0), and resuspended in Pett IV solution (1 M NaCl, 10 mM EDTA, pH 8.0). An equal volume of melted 2.0% low-melting-point agarose (Low Melt Preparative Grade; Bio-Rad Laboratories, Hercules, Calif.) was added to this suspension. The mixture was poured into an insert and chilled at 4°C for 20 min. The mixture was then treated at 37°C with 50 mg of lysozyme (Seikagaku, Tokyo, Japan)/ml in a lysis solution (1 M NaCl, 0.1 M EDTA [pH 8.0], 10 mM Tris-HCl, 0.5% Brij 58, 0.2% sodium deoxycholate, 0.5% Sarkosyl). After 1 h, the lysis solution was decanted and replaced with 1 mg of proteinase K (Wako Pure Chemical Industries, Osaka, Japan)/ml in ES solution (0.25 M EDTA [pH 8.0], 1% Sarkosyl) at 50°C for 48 h. The ES solution was decanted, and the plugs were placed in TE buffer (10 mM Tris-HCl [pH 8.0], 1 mM EDTA [pH 8.0]) containing 1 mM phenylmethylsulfonyl fluoride at room temperature for 4 h. The plugs were washed three times in TE buffer for 10 min at room temperature. For restriction endonuclease digestion, the plugs were incubated in 10 mM Tris-HCl-0.1 mM EDTA solution for 20 min at room temperature. This solution was decanted, and the plugs were incubated in enzyme...

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The Role of Antibiotics inCampylobacter pyloriAssociated Peptic Ulcer Disease

Cited by 86 publications

References 25 publications

Synthesis and Urease Inhibition Studies of Barbituric and Thiobarbituric Acid Derived Sulphonamides

Synthesis and Urease Inhibition Studies of Barbituric and Thiobarbituric Acid Derived Sulphonamides

Urease inhibitors from Indigofera gerardiana Wall.

Use of the Restriction Enzyme EcoRI for Pulsed-Field Gel Electrophoretic Analysis of Helicobacter pylori

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