Urease inhibitors from <i>Indigofera gerardiana</i> Wall.

Tariq, Shafiq Ahmad; Ahmad, Muhammad Nisar; Obaidullah,; Khan, Ajmal; Choudhary, M. Iqbal; Ahmad, Waqar; Ahmad, Manzoor

doi:10.3109/14756366.2010.528415

Cited by 21 publications

(15 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Urease Assay. Exactly 25 L of enzyme (Jack Bean Urease) solution and 5 L of test compounds (0.5 mM concentration) were incubated for 15 min at 30 ∘ C [9]. The aliquot was taken after 15 min and again incubated with 55 L of buffers containing 100 mM urea for 15 min at 30 ∘ C. Ammonia production was measured as a urease activity by indophenol method as described earlier [19].…”

Section: Extraction Of Total Sterolmentioning

confidence: 99%

See 1 more Smart Citation

Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study

Khan

Tariq

et al. 2014

Ulcers

Self Cite

View full text Add to dashboard Cite

Artimisia scoparia has been used in the treatment of different disorders including ulcers. The current study was therefore designed to investigate the aerial parts of Artemisia scoparia (crude extract, total sterol and flavonoidal contents, and aqueous fraction) for its urease inhibitory potential. The crude of the plant evoked marked attenuation on urease activity, when tested in various concentrations with IC50 values of 4.06 mg/ml. The inhibitory potential was further augmented in the aqueous fraction (IC50: 2.30 mg/ml) of the plant. When the total sterol and flavonoidal contents were challenged against urease, both showed concentration dependent activity; the latter showed maximum potency with IC50 values of 8.04 and 2.10 mg/ml, respectively. In short, the aerial parts of the plant demonstrated marked antagonism on urease and thus our study validated the traditional use of Artemisia scoparia in the treatment of ulcer.

show abstract

Section: Extraction Of Total Sterolmentioning

confidence: 99%

“…Indeed more effective and potent compounds are required with a whole new level of safety and specificity. In this regard, urease inhibitors have gained tremendous attention over the years which resulted into the discovery of numerous inhibitors [5,[7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study

Khan

Tariq

et al. 2014

Ulcers

Self Cite

View full text Add to dashboard Cite

show abstract

“…Almost 76% of all components present in the EO from lemongrass (Cymbopogon schoenanthus) are monoterpene aldehydes, and mainly two isomers, neral (29) and geranial (33). Geraniol and its acetate are the next two components of this EO that are worth mentioning.…”

Section: Analysis Of Essential Oilsmentioning

confidence: 99%

The In Vitro Activity of Essential Oils against Helicobacter Pylori Growth and Urease Activity

et al. 2020

View full text Add to dashboard Cite

The anti-H. pylori properties of 26 different commercial essential oils were examined in vitro by MIC (minimal inhibitory concentration) determination for the reference strain H. pylori ATCC 43504. We selected 9 essential oils with different anti-Helicobacter activities and established their phytochemical composition and urease inhibition activities. Phytochemical analysis of the selected essential oils by GC-MS method and antioxidant activity were performed. The phenol red method was used to screen the effect of essential oils on urease activity expressed as IC50 (the half of maximal inhibitory concentration). The most active essential oils, with MIC = 15.6 mg/L, were thyme, lemongrass, cedarwood and lemon balm oils; MIC = 31.3 mg/L—oregano oil; MIC = 62.5 mg/L—tea tree oil; MIC = 125 mg/L—pine needle, lemon and silver fir oils with bactericidal effect. Urease activity was inhibited by these oils with IC50 ranged from 5.3 to > 1049.9 mg/L. The most active was cedarwood oil (IC50 = 5.3 mg/L), inhibiting urease at sub-MIC concentrations (MIC = 15.6 mg/L). The statistical principal component analysis allowed for the division of the oils into three phytochemical groups differing in their anti-H. pylori activity. To summarize, the activity in vitro of the five essential oils silver fir, pine needle, tea tree, lemongrass, and cedarwood oils against H. pylori was found in this paper for the first time. The most active against clinical strains of H. pylori were cedar wood and oregano oils. Moreover, cedarwood oil inhibited the urease activity at subinhibitory concentrations. This essential oil can be regarded as a useful component of the plant preparations supporting the eradication H. pylori therapy.

show abstract

“…SAR studies revealed that aliphatic chain is responsible for activities of these compounds. In addition, both compounds were found to be competitive inhibitors of urease . The chloroform and ethyl acetate‐soluble fractions of the roots of Ranunculus repens led to the isolation of dalbergiophenol ( 12 ).…”

Section: Pure Compounds Isolated From Plants With Anti‐urease Activitymentioning

confidence: 99%

Plant‐Derived Urease Inhibitors as Alternative Chemotherapeutic Agents

Hassan

Žemlička²

2016

Archiv der Pharmazie

View full text Add to dashboard Cite

Inhibition of the metalloenzyme urease has important pharmacologic applications in the field of antiulcer and antigastric cancer agents. Urease is involved in many serious infections caused by Helicobacter pylori in the gastric tract as well as by Proteus and related species in the urinary tract. Although numerous studies have described several novel urease inhibitors (UIs) used for the treatment of gastric and urinary infections, all these compounds have exhibited severe side effects, toxicity, and instability. Therefore, to overcome such problems, it is necessary to search for new sources of UIs, such as natural products, that provide reduced side effects, low toxicity, greater stability, and bioavailability. As limited studies have been conducted on plant-derived UIs, this paper aims to highlight and summarize the most promising compounds isolated and identified from plants, such as terpenoids, phenolic compounds, alkaloids, and other substances with inhibitory activities against plant and bacterial ureases; these are in vitro and in vivo studies with an emphasis on structure-activity relationship studies and types of inhibition that show high and promising levels of anti-urease activity. This will aid medicinal chemists in the design and synthesis of novel and pharmacologically potent UIs useful for the development of antiulcer drugs.

show abstract

Urease inhibitors from Indigofera gerardiana Wall.

Cited by 21 publications

References 9 publications

Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study

Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study

The In Vitro Activity of Essential Oils against Helicobacter Pylori Growth and Urease Activity

Plant‐Derived Urease Inhibitors as Alternative Chemotherapeutic Agents

Contact Info

Product

Resources

About