The Role of Anticoagulation in the Measurement of Platelet Volumes

Abstract

The effect of anticoagulation on platelet size stability was studied using blood collected in seven different anticoagulants and stored at room temperature for up to eight hours. The mean platelet volume (MPV) value was most stable in blood collected in 15% ACD and ACD/Na2EDTA. In blood collected in Na2EDTA, K3EDTA, or 11.9% ACD, there was an increase in MPV in the first two hours, after which the MPVs remained stable up to eight hours. Sodium citrate and heparin proved unreliable for the measurement of platelet volume. Platelet counts were stable (less than 5% variation) in all anticoagulants except heparin, which had 16% variation for the eight hours of study. Simultaneously, RBC counts and mean corpuscular volume (MCV) measurements were stable in all seven anticoagulants, with sodium citrate producing the most variation. A negative correlation was observed between MCV and pH of the anticoagulated blood. WBC counts showed less than 3% variation in all anticoagulants except sodium citrate and heparin. Separate experiments demonstrated that electrolyte composition, pH, tonicity, and method of calcium chelation all influenced the stability of the MPV. Of the anticoagulants studied, ACD/Na2 EDTA appeared to provide the best conditions of anticoagulation for both routine clinical and research laboratory measurement of the MPV. It inhibited platelet activation but left the platelets in their normal discoid shape. Platelets could be removed from the anticoagulant and studied in functional assays for up to eight hours after blood drawing. Both platelet counts and MPVs remained stable in blood collected in ACD/Na2 EDTA anticoagulant for up to eight hours at room temperature. In 52 volunteers studied, an inverse correlation (r = -0.72, P less than 0.001) was observed between platelet count and MPV, suggesting that the circulating platelet mass may be a more important indicator of platelet homeostasis than either the platelet count or the mean platelet volume alone.

Prognostic value of platelet indices after acute myocardial infarction treated with primary percutaneous coronary intervention

“…Lack of inclusion of any data on beta-blockers or statins intakeas known predictors of mortality beyond hospital phase -makes the results of multivariate analysis not as informative as possible [20]. EDTA is recognized to provide the best conditions of anticoagulation for both routine clinical and research laboratory measurement of platelet volume [21,22]. However, previous studies reported platelet swelling when EDTA was used as an anticoagulant.…”

A Role for Changes in Platelet Production in the Cause of Acute Coronary Syndromes

“…35 The relationship between these two parameters appears to remain constant over a long period of time in steady-state platelet production. 36 However, others 37,38 have demonstrated that, during stimulated thrombopoiesis, there is an increase both in platelet count and in volume. Corash et al 32 found an increase in the volume of circulating platelets 8 hours after induction of thrombocytopenia following antiserum.…”

Mean platelet volume as diagnostic and therapeutic marker of risk and prognosis of heart disease

“…43 Na-EDTA as an anticoagulant, results in less pronounced swelling than K-EDTA. 44 More so, sodium citrate results in more distinct swelling in low concentration (0.12 mmol/mL, ratio 9:1, blood to citrate) and almost no swelling in high concentration citrate (0.12 mmol/mL, ratio 4:1, blood to citrate). 45 …”