The role of Atg7-mediated autophagy in ionizing radiation-induced neural stem cell damage

Shi, Weihua; Liu, Wei; Ma, Jiayan; Lu, Jiawei; Yang, Xiao; Wang, Jingdong; Cao, Jianping; Tian, Ye; Yang, Huiling; Zhang, Liyuan

doi:10.1016/j.gene.2020.144485

Cited by 14 publications

(11 citation statements)

References 42 publications

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“…Similarly, genes encoding heat shock proteins are activated under acute radiation and provide an adaptive response to stress [38,39,85]. We also expected changes in the expression of genes of autophagy and ER stress response-since, in studies on rodent and human cell lines, a connection between these mechanisms and the response to radiation has been established [86][87][88][89][90]. However, their changes in our study were small and inconsistent.…”

Section: Discussionmentioning

confidence: 76%

Tissue-Specific Knockdown of Genes of the Argonaute Family Modulates Lifespan and Radioresistance in Drosophila Melanogaster

Proshkina¹,

Yushkova²,

Koval³

et al. 2021

Preprint

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Small RNAs are essential for the coordination of many cellular processes, including the regulation of gene expression patterns, the prevention of genomic instability, and the suppression of mutagenic transposon activity. These processes determine aging, longevity, and sensitivity of cells and an organism to stress factors (particularly, ionizing radiation). The biogenesis and activity of small RNAs are provided by proteins of the Argonaute family. These proteins participate in the processing of small RNA precursors and the formation of an RNA-induced silencing complex. However, the role of Argonaute proteins in the regulation of lifespan and radioresistance remains poorly explored. We studied the effect of knockdown of Argonaute genes (AGO1, AGO2, AGO3, piwi) in various tissues on the Drosophila melanogaster lifespan and survival after the γ-irradiation at a dose of 700 Gy. In most cases, these parameters were reduced or did not change significantly in flies with tissue-specific RNA interference. Surprisingly, piwi knockdown in both the fat body and the nervous system caused a lifespan increase. But changes in radioresistance depended on the tissue in which the gene was knocked out. In addition, analysis of changes in retrotransposon levels and expression of stress response genes allowed us to determine associated molecular mechanisms.

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Section: Discussionmentioning

confidence: 76%

Tissue-Specific Knockdown of Genes of the Argonaute Family Modulates Lifespan and Radioresistance in Drosophila Melanogaster

Proshkina¹,

Yushkova²,

Koval³

et al. 2021

Preprint

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“…Basal autophagy has emerged as a core protective mechanism against CNS aging and neurodegenerative diseases [ 49 , 50 , 51 ]. Defects in autophagy impair the proliferation and differentiation of stem cells, and decrease neurogenesis [ 51 , 52 ]. Beclin-1 and LC3II/I are commonly used as autophagy-related markers [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Fe3O4 Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in D-Galactose-Induced Aged Mice

Xia

Gao

Peng

et al. 2022

IJMS

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Aging is a process associated with blood–brain barrier (BBB) damage and the reduction in neurogenesis, and is the greatest known risk factor for neurodegenerative disorders. However, the effects of Fe3O4 nanozymes on neurogenesis have rarely been studied. This study examined the effects of Fe3O4 nanozymes on neuronal differentiation in the dentate gyrus (DG) and BBB integrity of D-galactose-induced aged mice. Long-term treatment with Fe3O4 nanozymes (10 μg/mL diluted in ddH2O daily) markedly increased the doublecortin (DCX) immunoreactivity and decreased BBB injury induced by D-galactose treatment. In addition, the decreases in the levels of antioxidant proteins including superoxide dismutase (SOD) and catalase as well as autophagy-related proteins such as Becin-1, LC3II/I, and Atg7 induced by D-galactose treatment were significantly ameliorated by Fe3O4 nanozymes in the DG of the mouse hippocampus. Furthermore, Fe3O4 nanozyme treatment showed an inhibitory effect against apoptosis in the hippocampus. In conclusion, Fe3O4 nanozymes can relieve neuroblast damage and promote neuroblast differentiation in the hippocampal DG by regulating oxidative stress, apoptosis, and autophagy.

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“…NSC is a kind of undifferentiated stem cell that are defined by their replicative potential and long-term self-renewal, as well as the ability to differentiate into multiple neuronal and glial cell types [15]. Several studies have demonstrated that radiation treatment increased the apoptosis of NSC and inhibited the self-renewal and differentiation potential of NSC [16,17]. However, the mechanism of radiation treatment affecting NSC is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

Differentially expressed circRNA and mRNA profiles of neural stem cells with radiation irradiation

Cai¹,

Wang²,

Xie³

et al. 2020

Preprint

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Background Spine metastasis is common but highly problematic in clinical oncology practice. Radiotherapy plays an important role in the treatment of spine metastasis, but it at the same time damages the nervous tissue, especially the neural stem cell (NSC), and leads to radiation induced myelopathy. Circular RNA (circRNA) is a kind of non-coding RNA which responses to external stimulus and regulates cellular functions. However, the mechanism of radiotherapy affecting NSC and the role of circRNA in this process are still unclear. Methods The circRNA and mRNA of NSC treated with radiation or not were detect using next-generation sequencing. RT-PCR assays were preformed to confirm the sequencing results and the feature of differentially expressed circRNA. Bioinformation analyses were conducted to identified the critical circRNA and mRNA, as well as the enriched functions and pathways. Moreover, a circRNA-miRNA-mRNA network was constructed to investigate the possible regulatory mechanism. Results A total of 421 differentially expressed circRNA and 1602 differentially expressed mRNA of NSC were identified after radiotherapy. The GO and KEGG analysis of the differentially expressed mRNA as well as the host genes of the differentially expressed circRNA were performed and several key signal pathways such as MAPK signal pathway were identified. Moreover, a circRNA-miRNA-mRNA network focusing on MAPK signal pathway was shown and predicted that chr5:127160496|127165240 could be the critical circRNA in the regulatory mechanism of radiation treated NSCs. Conclusion Our finding showed the differentially expressed circRNA and mRNA profiles of NSC after radiotherapy, suggesting that circRNA may contribute to the pathogenesis of radiation induced myelopathy.

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The role of Atg7-mediated autophagy in ionizing radiation-induced neural stem cell damage

Cited by 14 publications

References 42 publications

Tissue-Specific Knockdown of Genes of the Argonaute Family Modulates Lifespan and Radioresistance in Drosophila Melanogaster

Tissue-Specific Knockdown of Genes of the Argonaute Family Modulates Lifespan and Radioresistance in Drosophila Melanogaster

Fe3O4 Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in D-Galactose-Induced Aged Mice

Differentially expressed circRNA and mRNA profiles of neural stem cells with radiation irradiation

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