The role of B-1 lymphocytes in antitumor immunity in patients with gastric cancer

Chulkova, S. V.; Шолохова, Е. Н.; Грищенко, Н. В.; Рябчиков, Д. А.; Гривцова, Л. Ю.; Bazin, I.; Тупицын, Н. Н.

doi:10.17650/1726-9784-2018-17-4-64-70

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…Субпопуляции В1-клеток способны дифференцироваться в антителопродуцирующие формы без стимуляции антигеном по так называемому тимуснезависимому пути, что сопровождается синтезом преимущественно IgМ [29]. Следует подчеркнуть, что В1-клетками секретируется примерно половина сывороточного IgМ [32]. Этот вид ответа не сопровождается формированием клеток памяти.…”

Section: обзоры литературыunclassified

“…С учетом угнетения тимопоэза до трансплантации этот путь оказывается значительным в формировании адекватного иммунного ответа у реципиента на вирусные и бактериальные патогены. Не следует забывать, что антитела класса М играют ключевую роль в индукции апоптоза опухолевых клеток [32].…”

Section: обзоры литературыunclassified

“…В первую очередь нормализуется синтез IgM. Антитела класса M являются наиболее ранними в иммуногенезе, и многие из антител полиспецифичны, имея низкую аффинность, взаимодействуют с несколькими антигенами [32].…”

Section: Fig 2 B-cells Count After Allogeneic Hematopoietic Stem Ceunclassified

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Reconstitution of B-Cells Immunity After Allogeneic Hematopoietic Stem Cell Transplantation

Chulkova

Субботина

Сидорова

et al. 2020

Rossijskij bioterapevtičeskij žurnal

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The restoration of B-cell immunity is a key component of the success of allogeneic hematopoietic stem cell transplantation. In most cases, the restoration of B-lymphopoiesis is a slow and often incomplete process, which is accompanied by a decrease in the tolerance of the recipient to bacterial, viral, fungal pathogens. This process is influenced by a number of factors that determine its effectiveness and pace. It is important to restore not only the size of the B-cell population, but also their functional usefulness. The article provides an analysis of modern literature data on the significance of the restoration of B-cell immunity after allogeneic hematopoietic stem cell transplantation, a review of the main factors affecting the process of B-lymphopoiesis, and their prognostic component.

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Section: обзоры литературыunclassified

Section: Fig 2 B-cells Count After Allogeneic Hematopoietic Stem Ceunclassified

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Reconstitution of B-Cells Immunity After Allogeneic Hematopoietic Stem Cell Transplantation

Chulkova

Субботина

Сидорова

et al. 2020

Rossijskij bioterapevtičeskij žurnal

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Features of peripheral blood B-cell subset phenotype are associated with clinical outcome of widespread purulent peritonitis

Belenyuk

Савченко

Борисов

et al. 2020

Russian Journal of Infection and Immunity

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The aim of the study was to investigate the phenotypic consiston of peripheral blood B-lymphocytes in patients with widespread purulent peritonitis (WPP) in the dynamics of postoperative treatment depending on the outcome of the disease. 52 patients with acute surgical diseases and injuries of the abdominal organs complicated by WPP were examined. Blood sampling was performed before surgery (preoperative period), as well as on the 7th, 14th and 21st day of the postoperative period. All patients with WPP were divided into two groups in depending on the outcome of the disease in the postoperative period: patients with a favorable outcome of the disease (n = 34), patients with an unfavorable outcome (n = 18). 68 healthy people were examined as a control. The phenotype of blood B-lymphocytes was studied by flow cytometry using direct immunofluorescence of whole peripheral blood with monoclonal antibodies. It was established that in the preoperative period in patients with WPP against the background of a low absolute level of B-lymphocytes in the blood, a decrease in the content of B1 cells relative to control values was detected. Moreover, a higher number of activated (according to CD23) B1 lymphocytes in the blood is observed in the patients with an unfavorable outcome of WPP than in the patients with a favorable outcome of the disease. The dynamics of changes in the content of B-lymphocytes in the observed period after surgery (7-21 days) varies significantly in depending on the outcome of the disease. There is a decrease in the number of most subpopulations of B-lymphocytes (including activated cells) in patients with a favorable outcome of WPP during the postoperative period, whereas the levels of various fractions of B-cells in patients with an unfavorable outcome of the disease in the postoperative period practically do not change. The number of total B-lymphocytes, naive B-cells and B2-lymphocytes non-expressing and expressing the CD23 receptor in patients with an unfavorable outcome of WPP were higher in during the entire postoperative period than in patients with a favorable outcome of the disease. The content of some other fractions of B-lymphocytes in patients with an unfavorable outcome was also higher than with a favorable outcome of WPP but only at certain stages of postoperative treatment. It is assumed that the established features in the levels of B-lymphocytes in the blood in depending on the outcome were determined by the fact that the patients with an unfavorable outcome of WPP had reduced the level of cell migration in the framework of the development of the immune response as well as a reduced level of sensitivity of the immune system to postoperative antibiotic therapy.

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The relationship of lymphoid populations (infiltration) of the primary tumor with bone marrow immune responses in patients with breast cancer

Chulkova

Шолохова

Poddubnaya

et al. 2023

Rossijskij bioterapevtičeskij žurnal

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Backgraund. Currently, immunotherapy is firmly established in the standard of cancer treatment. The basis for the appointment of immunotherapy are immunological tumor markers, which include lymphoid infiltration, a detailed study of which has received increasing attention in the last decade. An undoubted interest is the study of lymphoid infiltration, not only depending on the morpho-clinical parameters of breast cancer (BC), but also on the immune system of the bone marrow.Aim. To evaluate the infiltration of the primary tumor by lymphocytes depending on the morpho-clinical characteristics of BC and immune responses in the bone marrow.Materials and methods. This study included 125 patients with BC who received treatment at the “N.N. Blokhin National Medical Research Center of Oncology” of the Ministry of Health of Russia. Tumor stage II was prevailed, а moderate degree of differentiation (G2) was more often noted. The luminal BC – 67 %, non-luminal – 33 %. Immunophenotyping of the primary tumor: cryostat sections, ZEISS Axioscope luminescent microscope (Zeiss AG, Germany). CD45+, CD38+, T- and B-cell infiltration were assessed. Bone marrow: CD3+, CD4+, CD8+, CD19+, CD16+, CD56+ lymphocytes and their subpopulations were studied (FACSCanto II flow cytometer, Kaluza Analysis v2.1 program (Beckman Coulter, USA)).Results. CD45+ infiltration was noted in 50.5 % of cases (severe in 30 %, moderate – 26.4 %). CD8+ cells significantly infiltrated the tumor in 21.4 % of cases. CD38+ infiltration was observed in 40 %. In the non-luminal BC, severe CD45 infiltration was observed more frequently than in the luminal (33 % vs 26 %). CD38+ infiltration is expressed in non-luminal BC (p = 0.016). CD45+ infiltration was positively correlated with earlier stages (p = 0.071) more pronounced in infiltrative ductal BC, than in lobular BC: 59.2 % vs 20 % (p = 0.05). The content of CD45RO+cells in bone marrow in the luminal BC is higher than in the non-luminal: 37.3 ± 2.3 % vs 28 ± 2.8 % (p = 0.04). The number of CD19+CD38+ cells, on the contrary, is less: 24.2 ± 2 % vs 34.8 ± 6 % (p = 0.041). Tumor-infiltrating lymphocytes highly correlated with bone marrow lymphoid populations: CD38+ cells with NK-bone marrow cells; CD4+ cells with the B-precursors; CD8+cells with the B1-lymphocytes.Conclusion. Lymphoid infiltration of BC is associated with stage, tumor size, histological type and biological subtype. Intratumoral populations CD38+, CD4+, CD3+, CD8+ cells are in a negative correlation with bone marrow lymphoid populations.

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The role of B-1 lymphocytes in antitumor immunity in patients with gastric cancer

Cited by 5 publications

References 19 publications

Reconstitution of B-Cells Immunity After Allogeneic Hematopoietic Stem Cell Transplantation

Reconstitution of B-Cells Immunity After Allogeneic Hematopoietic Stem Cell Transplantation

Features of peripheral blood B-cell subset phenotype are associated with clinical outcome of widespread purulent peritonitis

The relationship of lymphoid populations (infiltration) of the primary tumor with bone marrow immune responses in patients with breast cancer

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