IL-6, or cytokines of the IL-6 family using gp130 as transducer chain receptor, have been suggested to play a role in certain B lymphoid neoplasia. The presence of cell membrane gp80 and gp130 IL-6 receptors was studied in 98 patients with various leukaemia and non-Hodgkin's malignant lymphoma using flow cytofluorometry and immunohistology. Except neoplasia of immature B cells which expressed neither of the receptors, the majority of B cell tumours expressed one or both of them, mantle cell lymphoma being found to express the highest density of receptors. Using IL-6-dependent XG myeloma cell lines and mAb recognizing various gp80 and gp130 functional epitopes, it has been shown that IL-6 activation leads to a modified expression of some epitopes. In particular, the decrease or the disappearance of a gp130 epitope called A1 signed gp130 dimerization which is the first step of the gp130 activation pathway. Gp80 and gp130 epitope analysis was achieved in 17 of the patients. In four, an epitope phenotype compatible with a cytokine-induced activation was found. The cells of five B-CLL patients which expressed both gp80 and gp130 receptors were incubated with IL-6 to induce activation. In three of the cases they were found to rearrange their receptors in activated forms but not in the two others, showing that cells able to be activated or not can be found. These results confirm that gp130 signalling might play an important role in the pathogenesis of certain B cell neoplasia.

В1-лимфоциты-субпопуляция В-клеток, на долю которой в селезенке приходится 5 % от общего числа В-клеток. В1-лимфоциты секретируют преимущественно IgM, который играет важную роль в индукции апоптоза опухолевых клеток. Спленэктомия с целью адекватной лимфодиссекции при раке желудка вызывает выраженные и длительные иммунологические нарушения. Это в первую очередь затрагивает субпопуляцию В1а-лимфоцитов, которая обеспечивает тимуснезависимый иммунный ответ. Цель исследования-изучить особенности В-клеточного звена иммунитета у больных раком желудка. Материалы и методы. Проанализирован субпопуляционный состав В-лимфоцитов периферической крови больных раком желудка, подвергшихся хирургическому лечению методом проточной цитометрии (Facs Can, программа Lysys II и FacsCanto II, программа Facs Diva). Клетки окрашивались одномоментно тремя моноклональными антителами, меченными различными флуорохромами. Результаты. Полученные данные демонстрируют нарушение состава субпопуляций В-клеток. В группе больных со стандартной D2-лимфодиссекцией и спленэктомией на дооперационном этапе и спустя 3 мес после операции выявлена достоверная корреляция между относительным количеством В-лимфоцитов (p = 0,018), CD5+В-лимфоцитов (p = 0,012) и количеством CD19+CD38+-клеток (p = 0,035). Кроме того, после хирургического лечения значительно возрос процент CD5+В-лимфоцитов-с 12,9 до 21,8 %, тогда как суммарное количество CD19+ лимфоцитов и CD19+СD21+ клеток снижалось. Заключение. У больных опытной группы может наблюдаться снижение антителoпродукции, ослабление как общего, так и противоопухолевого иммунитета.

Marginal zone lymphoma (LMZ) accounts for 5–15% of all NHL in Europe. This option includes splenic (0.7%), nodal (2.4%) and extranodal (MALT-Mucosa-Associated Limphoid-Tissue) LMZ −5%. Extranodal variants of MALT lymphomas can occur in any organ due to chronic antigenic stimulation. The most frequent localization associated with Helicobacter pylori (HP) infection is the stomach - 30%. The gastrobiopsy material of 115 patients with lymphoid cell infiltrates in the gastric mucosa was studied, a complex of morphological diagnostic criteria for MALT gastric lymphoma for gastrobiopsy was developed based on a combination of histological and immunohistochemical characteristics of tumor cells, the nature of their growth. It is known that the mandatory initial therapy for local stages of HP-positive MALT lymphoma of the stomach is the eradication of HP. 68 patients with stages I – II of gastric MALT lymphomas were observed. Anti HP therapy resulted in 87.8% of complete remissions, with a median duration of 51 months. The median time to the onset of HP- eradication was 3 months, and the median time to the implementation of the antitumor process was 5.5 months. With a median follow-up of 58 months, the median overall and relapse-free survival was not achieved: 10-year OS - 100%, 10-year RFS - 92. 3%.

The activities of membrane-bound proteases: ƒÁ-glutamyltranspeptidase (ƒÁ-GTP), microsomal alanyl aminopeptidase (mAAP, formerly APM), glutamyl-aminopeptidase (EAP, formerly APA) and dipeptidyl peptidase IV (DPP-IV)-were examined in 11 cases with non-Hodgkin's malignant lymphomas (NHL), in 7 cases with Hodgkin's disease (HD) and 8 cases with reactive follicular hyperplasia (RFH) by means of enzyme histochemical methods. The results indicated that there was correlation between grade malignancy of NHL and ƒÁ-GTP activity: marker with which to predict drug-and irradiation resistance. Moreover, mAAP-and EAP pattern in the lymphoid cells may be useful in defining in lymph nodes the metastatic tumour cells with strong activities of these enzymes.