The role of bone marrow‐derived mesenchymal stem cells in treating formocresol induced oral ulcers in dogs

Abstract

J Oral Pathol Med (2010) 39: 281–289 Background: Mesenchymal stem cells (MSCs), a subpopulation of adult somatic stem cells, are an attractive stem cell source in regenerative medicine because of their multipotentiality. In this study, the effects of MSCs transplantation on oral ulcer healing were examined. Methods: Mesenchymal stem cells were isolated from bone marrow aspirates of dogs by dish adherence and expanded in culture. Oral ulcers were induced by topical application of formocresol in the oral cavity of dogs. Either autologous MSCs or vehicle (saline) was injected around the ulcer. The healing process of the ulcer was monitored clinically and histopathologically. Gene expression of vascular endothelial growth factor (VEGF) was detected in MSCs by reverse transcription‐polymerase chain reaction. Expression of VEGF and collagen genes was detected in biopsies from all ulcers. Results: Mesenchymal stem cells expressed mRNA for VEGF MSCs transplantation significantly accelerated oral ulcer healing compared with controls. There was increased expression of both collagen and VEGF genes in MSCs‐treated ulcers compared with controls. Conclusion: Mesenchymal stem cells transplantation may help accelerate oral ulcer healing, possibly through the induction of angiogenesis by VEGF together with increased intracellular matrix formation as detected by increased collagen gene expression.

Adipose Stem Cells as Alternatives for Bone Marrow Mesenchymal Stem Cells in Oral Ulcer Healing

“…Following transfection the cells were incubated at 37°C in humidified air (5% CO2) for 2 h. The transfection medium was then removed and the cells were incubated for an additional 48 h in complete medium (2 ml per well). For imaging GFP autofluorescence of MSCs, unstained slides were directly analysed and green autofluoresence detected by inverted fluorescence microscopy (Leica DM IRB, Leica, Wetzlar, Germany) (15,16).…”

Stem cell therapy: A novel treatment approach for oral mucosal lesions

“…There was increased expression of both collagen and VEGF (vascular endothelial growth factor) genes in MSCs-treated ulcers compared with controls. [ 17 ] Similar results were seen by Aziz Aly et al . in a study on formocresol induced oral ulcers in dogs using BM and adipose-derived stem cells.…”

VEGF165 expressing bone marrow mesenchymal stem cells differentiate into hepatocytes under HGF and EGF induction in vitro

“…Some studies have shown that MSCs could also achieve the biological effects by secreting VEGF. VEGF secreted by MSCs has been used in gastric ulcer and oral ulcer healing (El-Menoufy et al 2010;Hayashi et al 2008), in murine ischemic limbs leading to an increase in vessel density (Hoffmann et al 2010), in inhibiting the apoptosis of lung cells (Zhen et al 2010), in facilitating neurological function (Deng et al 2010), enhancing the repair and regeneration of critical sized bone defects (Kanczler et al 2010) and improving cardiac function in failing rat hearts (Tao et al 2009). Therefore, the VEGF 165 gene is the best target gene in gene therapy for promoting angiogenesis.…”