The Role of Citrus Nobiletin on Oxidative Stress Levels and Superoxide Dismutase Activities in Metastatic Castration-Resistant Prostate Cancer

Abstract

Nobiletin (NOB) is a polymethoxylated flavone. It has multiple biologic activities that can modulate oxidative stress in many cancer types. However, there is no study in the literature that has examined the effects of NOB on oxidative stress levels in Metastatic Castration-Resistant Prostate Cancer (MCRPC) yet. Motivated from this gap, we investigated the impact of NOB on oxidative stress and superoxide dismutase (SOD) enzyme activities in MCRPC as a preliminary study. For this purpose, PC-3 and HUVEC cells were used to determine the effects of NOB on the amount of Malondialdehyde (MDA), hydrogen peroxide (H2O2), and proline as well as SOD enzyme activities. NOB potentially induced SOD enzyme activities but the level of MDA, H2O2, and proline decreased after incubation with NOB in PC-3 cells (p<.05 and p<.001 were considered statistically significant). Our results confirmed that NOB acted as a protective agent for cancer cells and could selectively regulate oxidant status in MCRPC cells. Consequently, these preliminary findings provide better insight into the role of citrus NOB on oxidative stress levels and antioxidant enzyme activities in MCRPC. Additionally, there is a need to elucidate the molecular mechanisms of this cytoprotective effect of NOB as a potential chemotherapeutic agent.

Poly I:C'nin İndüklediği TLR3 Aktivasyonunun Prostat Kanseri Hücreleri Olan PC-3 (Hormona Duyarsız) ve LNCaP'ın (Hormona Duyarlı) Oksidatif Stres Düzeyine Etkileri

“…39 Although there is much to explain about the proline system, the data obtained about its role in cancer. [40][41][42][43] These data support the increase (2.3-fold) in proline level over 24 hours in LNCaP cells incubated with Poly I:C. However, no proline level changes were observed in PC-3 cells treated with Poly I:C after 6 and 24 hours.…”

A systematic review of antiproliferative and antitumour activity of earthworm extracts

“…Because chemotherapeutic treatments cause systemic toxicity and impair life quality (Chabner & Roberts 2005, potential chemotherapeutic agents can be selected from natural products with less toxic effects. Extracts obtained from natural products have antioxidant effects with their oxidative radical scavenging feature, an adjunct cancer treatment (Yuksel & Deveci Ozkan 2021).…”