The role of dermatopontin in cell adhesion in bovine placenta during early-mid pregnancy and parturition – Pilot study

Wawrzykowski, Jacek; Jamioł, Monika; Kankofer, Marta

doi:10.1016/j.theriogenology.2021.05.014

Cited by 6 publications

(9 citation statements)

References 25 publications

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“…EPAS1, or hypoxia-inducible factor-2α (HIF-2α), is a key factor in hypoxia ( 26 ). Changes in enzymes or factors caused by hypoxia act mainly through HIF-2α and regulate the expression level of its own and downstream target genes, leading to rapid and accurate responses in tissues and organs ( 27 ). The DE gene EPAS1 was negatively correlated with the most DE miRNA, miR-150_R-1 ( p = 1.16 × 10 −6 ).…”

Section: Discussionmentioning

confidence: 99%

miRNA-150_R-1 mediates the HIF-1/ErbB signaling pathway to regulate the adhesion of endometrial epithelial cells in cows experiencing retained placenta

Li²,

Wang³

et al. 2022

Front. Vet. Sci.

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Retained placenta (RP) refers to reproductive disorders caused by the failure of fetal membranes to be expelled 12 h after delivery in dairy cows. Postpartum adhesion of the fetal membranes to the uterus causes diseases such as mastitis or endometritis, which threatening the profitability of the dairy industry. Emerging evidence suggests that micro RNAs (miRNAs) play crucial roles in various processes, such as the occurrence and progression of fetal membranes discharge. However, the molecular mechanisms of miRNAs in RP remain unknown. In this study, we performed RNA-sequencing to characterize the expression profiles of mRNAs and miRNAs in caudal vein blood samples of postpartum Holstein cows whose fetal membranes were discharged normally or retained to identify RP-related genes and evaluate their molecular mechanisms. We identified 44 differentially expressed miRNAs (19 upregulated and 25 downregulated) and 706 differentially expressed mRNAs (325 upregulated and 381 downregulated) in the RP group compared to the normal fetal membranes discharge group. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that differentially expressed mRNAs were mainly enriched in the extracellular matrix, cell adhesion, and autoimmunity-related biological processes or pathways. Further analyses using RNA-sequencing, a dual luciferase reporter system, quantitative reverse transcription-PCR, immunofluorescence, and western blotting verified that endothelial PAS domain protein 1 (EPAS1) is regulated by miR-150_R-1 in endometrial epithelial cells. We demonstrated the relationship between EPAS1 and RP and confirmed that EPAS1 is upregulated in the blood and placenta of cows that experience RP. Further, we proposed a model of the miRNA-mRNA negative regulatory network mediated by the HIF-1/ErbB signaling pathway to show its regulatory role in RP.

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Section: Discussionmentioning

confidence: 99%

miRNA-150_R-1 mediates the HIF-1/ErbB signaling pathway to regulate the adhesion of endometrial epithelial cells in cows experiencing retained placenta

Li²,

Wang³

et al. 2022

Front. Vet. Sci.

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“…Cell isolation was performed according to the protocol described by Hambruch et al (2010) with minor modifications described previously by Jamioł, Wawrzykowski, and Kankofer (2021); Jamioł, Wawrzykowski, Bulak, et al (2021); Wawrzykowski et al (2021). The epithelial layer of the tissue was digested with 0.25% trypsin solution (25‐054‐CI; Corning).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The same amount of protein (20 µg of total protein/line) was separated by 7.5% (for THBS1) and 12.0% SDS‐PAGE (for TGFβ1), as described previously (Wawrzykowski et al, 2021), and transferred to PVDF membranes (pb9320; Invitrogen) with Trans‐Blot® Turbo™ Transfer System (25 V, 30 min; Bio‐Rad) in accordance with the manufacturer's recommendations. WB procedure after the transfer was similar to previously described by our team (Franczyk et al, 2018; Jamioł, Wawrzykowski, Bulak, et al, 2021; Wawrzykowski et al, 2021). Membranes were blocked for 1 h in TBST (20 mmol/L Tris, 150 mmol/L NaCl, and 0.1% Tween 20; pH = 7.6) containing 4% low‐fat milk, and then incubated overnight at 4°C with 50 ng/mL THBS1 antibody (abx104797; Abbexa) or 1 mg/mL TGFβ1 antibody (abx008643; Abbexa).…”

Section: Methodsmentioning

confidence: 99%

“…Maternal and fetal parts were separated manually and analyzed in duplicates. The procedures for obtaining the placentomes after delivery were analogous as described earlier (Jamioł et al, 2020;Wawrzykowski et al, 2019Wawrzykowski et al, , 2021.…”

Section: Placental Tissue Samplesmentioning

confidence: 99%

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A pilot study on the relationship between thrombospondin‐1 (THBS1) and transforming growth factor beta1 (TGFβ1) in the bovine placenta during early mid‐pregnancy as well as parturition with normally released and retained placenta

Wawrzykowski,

Jamioł,

Kankofer

2023

Molecular Reproduction Devel

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During pregnancy, it is necessary to create appropriate conditions for the development of the placenta and the fetus. However, during parturition, the placenta must be separated and subsequently removed as soon as possible to not expose the female to the possibility of infection. In this study, the relationship between thrombospondin‐1 (THBS1) and transforming growth factor beta1 (TGFβ1) concentrations was described during bovine pregnancy (second, fourth, and sixth months; n = 3/each month), at normal parturition (NR) and parturition with fetal membrane retention (R). The presence of THBS1 and TGFβ1 was confirmed in bovine placental tissues of both maternal and fetal parts. Enzyme‐linked immunosorbent assay showed statistically significant differences (p < 0.05) in THBS1 concentrations (pg/mg protein) between examined parturient samples (maternal part: 5.76 ± 1.61 in R vs. 2.26 ± 1.58 in NR; fetal part: 2.62 ± 1.94 in R vs. 1.70 ± 0.23 in NR). TGFβ1 concentrations (pg/mg protein) were significantly lower (p < 0.05) in the retained fetal membranes compared to the released fetal membranes in the maternal part of the placenta (26.22 ± 7.53 in NR vs. 17.80 ± 5.01 in R). The participation of THBS1 in the activation of TGFβ1 in parturient bovine placental tissues leading to the normal release of fetal membranes may be suggested.

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Expression of cadherins and some connective tissue components in cow uterus and placenta during pregnancy

Sağsöz

Lïman

Akbalık

et al. 2022

Research in Veterinary Science

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The role of dermatopontin in cell adhesion in bovine placenta during early-mid pregnancy and parturition – Pilot study

Cited by 6 publications

References 25 publications

miRNA-150_R-1 mediates the HIF-1/ErbB signaling pathway to regulate the adhesion of endometrial epithelial cells in cows experiencing retained placenta

miRNA-150_R-1 mediates the HIF-1/ErbB signaling pathway to regulate the adhesion of endometrial epithelial cells in cows experiencing retained placenta

A pilot study on the relationship between thrombospondin‐1 (THBS1) and transforming growth factor beta1 (TGFβ1) in the bovine placenta during early mid‐pregnancy as well as parturition with normally released and retained placenta

Expression of cadherins and some connective tissue components in cow uterus and placenta during pregnancy

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