The role of dimethyl sulfoxide (DMSO) in ex-vivo examination of human skin burn injury treatment

Pielesz, Anna; Gawłowski, Andrzej; Biniaś, Dorota; Bobiński, Rafał; Kawecki, Marek; Klama-Baryła, Agnieszka; Kitala, Diana; Łabuś, Wojciech; Glik, Justyna; Paluch, Jadwiga

doi:10.1016/j.saa.2018.02.035

Cited by 16 publications

(6 citation statements)

References 28 publications

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“…The selection of LOI method for this type of research was based on similar research on protein fibers (e.g., wool) [22]. Basically, the LOI results for human skin are varied; they are different in terms of the type of skin damage (e.g., thermal or electric burn), the type of tissue: xenogeneic unburned skin, allogenic unburned full-thickness skin, burned human skin [23]. The results shown in work [2, 3] point to an increase in the LOI for samples treated with a mixture of antioxidants and a decrease in the LOI for samples treated with a mixture of antioxidants and DMSO solutions.…”

Section: Resultsmentioning

confidence: 99%

FT Raman spectroscopy in the evaluation of biomarkers of normal and pathological placenta tissue

et al. 2019

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The basic precondition of proper intrauterine growth is appropriate supply of nutrients transported through placenta. Placenta capacity in the scope of transportation is dependent on transport systems and the structure of the basement membrane and syncytiotrophoblast microvillous membrane. The present pilot study demonstrates preliminary results of the analysis of placenta structure in the course of selected pathologies by FT Raman spectroscopy analysis. The observed changes of the molecular structure in the so-called average spectra, independent of methodical processing, may be an indicator of the efficiency of transportation controlled by syncytiotrophoblast. In particular, an increase in the intensity of dispersion and transfer within the frequency of 3425–3300 cm −1 demonstrate the dynamics of the interaction in the scope of hydrogen bonds in healthy tissues. Changes in the molecular structure within the frequency of 950–750 cm −1 and conformational changes within disulphide bonds differentiate the healthy tissue from the pathological one. Changes in the molecular structure observed in the FTR spectra are a spectroscopic image of placenta functions in the course of various pathologies. They also document a complex goal of our research that is finding spectroscopic biomarkers of regular and pathological placental tissue.

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Section: Resultsmentioning

confidence: 99%

FT Raman spectroscopy in the evaluation of biomarkers of normal and pathological placenta tissue

et al. 2019

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“…It is important to remember that the extraction of RB was performed on the entire dermatomed skin without separating the epidermis from the dermis. Herein, DMSO was employed as lysing solvent, and it is recurrently used in biological studies because the solvent quickly penetrates and diffuses through biological membranes [ 69 ]. Furthermore, DMSO can dissolve the skin lipids or denature skin proteins.…”

Section: Resultsmentioning

confidence: 99%

“…In this case, the values recorded were a λmax 552.5 nm and FI 301.8 for RB-S, a λmax 560 nm and FI 329.7 for RB-C and a λmax 588 nm with a FI 403.4 in the case of RB-TF. We previously reported that DMSO dissolves skin lipids and, simultaneously, the lipids constituting the tested formulations [ 69 ]. The skin samples, spiked with RB-loaded formulations, were all submitted to the same extraction process described in Section 2.5 , which employed DMSO as a lysing solvent.…”

Section: Resultsmentioning

confidence: 99%

Fluorescence-Coupled Techniques for Determining Rose Bengal in Dermatological Formulations and Their Application to Ex Vivo Skin Deposition Studies

et al. 2023

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Rose Bengal (RB) is a fluorescent dye with several potential biomedical applications, particularly in dermatology. Due to RB’s poor physicochemical properties, several advanced delivery systems have been developed as a potential tool to promote its permeation across the skin. Nevertheless, no validated quantitative method to analyse RB within the skin is described in the literature. Considering RB exhibits a conjugated ring system, the current investigation proposes fluorescence-based techniques beneficial for qualitatively and quantitatively determining RB delivered to the skin. Notably, the development and validation of a fluorescence-coupled HPLC method to quantify RB within the skin matrix are herein described for the first time. The method was validated based on the ICH, FDA and EMA guidelines, and the validated parameters included specificity, linearity, LOD, LLOQ, accuracy and precision, and carry-over and dilution integrity. Finally, the method was applied to evaluate RB’s ex vivo permeation and deposition profiles when loaded into dermatological formulations. Concerning qualitative determination, multiphoton microscopy was used to track the RB distribution within the skin strata, and fluorescence emission spectra were investigated to evaluate RB’s behaviour when interacting with different environments. The analytical method proved specific, precise, accurate and sensitive to analyse RB in the skin. In addition, qualitative side-analytical techniques were revealed to play an essential role in evaluating the performance of RB’s dermatological formulation.

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“…41 The citing literature reports DMSO's superior efficacy as a topical treatment for thermal burns in humans and animals, with the application of up to 40% aqueous DMSO solution. 42,43 Further clinical trials are needed to validate the safety and efficacy in a larger population. However, the administration of DMSO has been proven to be a practical control of acute symptoms, a promotion of the healing process, and, most importantly, an effective preventive method for long-term damages to the patients' eyes and vision in all types of burns.…”

Section: Potential Application Of Dmso To Treat Eye Injuries Due To Chemical Burnsmentioning

confidence: 99%

Application of Dimethyl Sulfoxide as a Therapeutic Agent and Drug Vehicle for Eye Diseases

Hoang¹,

Nguyen²,

NGUYEN³

et al. 2021

Journal of Ocular Pharmacology and Therapeutics

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Dimethyl sulfoxide (DMSO) is an amphipathic molecule widely used as a solvent for water-insoluble substances, cryopreserving, and cell-biological therapies. It has known properties as an inducer of cellular differentiation, a free radical scavenger, and a radioprotectant. In addition, DMSO is used for its various therapeutic and pharmaceutical properties, such as anti-inflammatory, local and systemic analgesic, antibacterial, antifungal, antiviral, and membrane penetration enhancement agents. DMSO treatment can be given orally, intravenously, or topically for a wide range of indications. The administration of DMSO exhibits favorable outcomes in human eye diseases with low to none observed ocular or systemic ocular toxicity. Nevertheless, DMSO is an essential and nonpatentable potential therapeutic agent that remains underexplored and ignored by pharmaceutical developers and ophthalmologists. This current review takes data from experimental and clinical studies that have been published to substantiate the potential therapeutic efficacy of DMSO and stimulate the research of its application in clinical ophthalmology. Given that DMSO is inexpensive, safe, and easily formulated into therapeutic medicinal products and conventional ophthalmological drugs, this compound should be further explored and studied in the treatment of a variety of acute and chronic ocular disorders.

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The role of dimethyl sulfoxide (DMSO) in ex-vivo examination of human skin burn injury treatment

Cited by 16 publications

References 28 publications

FT Raman spectroscopy in the evaluation of biomarkers of normal and pathological placenta tissue

FT Raman spectroscopy in the evaluation of biomarkers of normal and pathological placenta tissue

Fluorescence-Coupled Techniques for Determining Rose Bengal in Dermatological Formulations and Their Application to Ex Vivo Skin Deposition Studies

Application of Dimethyl Sulfoxide as a Therapeutic Agent and Drug Vehicle for Eye Diseases

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