The role of echo-laparoscopy in abdominal surgery: five years’ experience in a dedicated center

Piccolboni, D.; Ciccone, F.; Settembre, A.; Corcione, Francesco

doi:10.1007/s00464-007-9382-x

Cited by 15 publications

(4 citation statements)

References 17 publications

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“…Laparoscopy is able to detect peritoneal carcinomatosis, while the addition of LUS allows definition of extent of the primary tumor, involvement of liver parenchyma and surrounding organs and infiltration (encasement or impingement) of vascular structures (12,13).…”

Section: Discussionmentioning

confidence: 99%

Laparoscopic ultrasound: a surgical �must� for second line intra-operative evaluation of pancreatic cancer resectability

Piccolboni¹,

Settembre²,

Angelini³

et al. 2015

GCHIR

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Section: Discussionmentioning

confidence: 99%

Laparoscopic ultrasound: a surgical �must� for second line intra-operative evaluation of pancreatic cancer resectability

Piccolboni¹,

Settembre²,

Angelini³

et al. 2015

GCHIR

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“…Ultrasonography is widely used during surgery to visualize lesions or critical vessels lying deep within organs, including the liver, 26 prostate and kidney, 27 and heart, 28 and in neurosurgery 29 and gynecological surgery. 30 These fields benefit from surgical navigation systems that combine laparoscopic vision with computed tomography, magnetic resonance imaging, or ultrasonographic images.…”

Section: Discussionmentioning

confidence: 99%

Small-Incision Laparoscopy-Assisted Surgery Under Abdominal Cavity Irrigation in a Porcine Model

Igarashi

Ishii

Aoe

et al. 2016

Journal of Laparoendoscopic & Advanced Surgical Techniques

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Background: Laparoscopic and robot-assisted surgeries are performed under carbon dioxide insufflation. Switching from gas to an isotonic irrigant introduces several benefits and avoids some adverse effects of gas insufflation. We developed an irrigating device and apparatus designed for single-incision laparoscopic surgery and tested its advantages and drawbacks during surgery in a porcine model.Materials and Methods: Six pigs underwent surgical procedures under general anesthesia. A 30-cm extracorporeal cistern was placed over a 5–6-cm abdominal incision. The abdomen was irrigated with warm saline that was drained via a suction tube placed near the surgical field and continuously recirculated through a closed circuit equipped with a hemodialyzer as a filter. Irrigant samples from two pigs were cultured to check for bacterial and fungal contamination. Body weight was measured before and after surgery in four pigs that had not received treatments affecting hemodynamics or causing diuresis.Results: One-way flow of irrigant ensured laparoscopic vision by rinsing blood from the surgical field. Through a retroperitoneal approach, cystoprostatectomy was successfully performed in three pigs, nephrectomy in two, renal excision in two, and partial nephrectomy in one, under simultaneous ultrasonographic monitoring. Through a transperitoneal approach, liver excision and hemostasis with a bipolar sealing device were performed in three pigs, and bladder pedicle excision was performed in one pig. Bacterial and fungal contamination of the irrigant was observed on the draining side of the circuit, but the filter captured the contaminants. Body weight increased by a median of 2.1% (range, 1.2–4.4%) of initial weight after 3–5 hours of irrigation.Conclusions: Surgery under irrigation is feasible and practical when performed via a cistern through a small abdominal incision. This method is advantageous, especially in the enabling of continuous and free-angle ultrasound observation of parenchymal organs. Adverse effects of abdominal irrigation need further assessment before use in humans.

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“…The preparation of the RNA’ samples for the microarray hybridization on the Affymetrix GeneChip Mouse Genome 430_2 array was described in 14 . Low-level analysis was performed by robust multiarray average (RMA) implemented using the RMA function of the Affymetrix package of the Bioconductor project 29,30 in the R programming language 31 . The low-level analysis for the BAMarray tool (v3.0) was performed using the MAS5 method as described in 14 and implemented using the corresponding function of the same Bioconductor package.…”

Section: Methodsmentioning

confidence: 99%

A transcriptomic study of Williams-Beuren syndrome associated genes in mouse embryonic stem cells

et al. 2019

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Williams-Beuren syndrome (WBS) is a relatively rare disease caused by the deletion of 1.5 to 1.8 Mb on chromosome 7 which contains approximately 28 genes. This multisystem disorder is mainly characterized by supravalvular aortic stenosis, mental retardation, and distinctive facial features. We generated mouse embryonic stem (ES) cells clones expressing each of the 4 human WBS genes (WBSCR1, GTF2I, GTF2IRD1 and GTF2IRD2) found in the specific delated region 7q11.23 causative of the WBS. We generated at least three stable clones for each gene with stable integration in the ROSA26 locus of a tetracycline-inducible upstream of the coding sequence of the genet tagged with a 3xFLAG epitope. Three clones for each gene were transcriptionally profiled in inducing versus non-inducing conditions for a total of 24 profiles. This small collection of human WBS-ES cell clones represents a resource to facilitate the study of the function of these genes during differentiation.

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The role of echo-laparoscopy in abdominal surgery: five years’ experience in a dedicated center

Cited by 15 publications

References 17 publications

Laparoscopic ultrasound: a surgical �must� for second line intra-operative evaluation of pancreatic cancer resectability

Laparoscopic ultrasound: a surgical �must� for second line intra-operative evaluation of pancreatic cancer resectability

Small-Incision Laparoscopy-Assisted Surgery Under Abdominal Cavity Irrigation in a Porcine Model

A transcriptomic study of Williams-Beuren syndrome associated genes in mouse embryonic stem cells

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