The role of endogenous gibberellin in seed and fruit development of tomato: Studies with a gibberellin‐deficient mutant

Groot, Steven P. C.; Bruinsma, J.; Karssen, C.M.

doi:10.1111/j.1399-3054.1987.tb02865.x

Cited by 95 publications

(62 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A physiological role for GAs in Arabidopsis seed development also is in agreement with previous results suggesting that GAs are required for normal seed growth in the gib1 tomato mutant (Groot et al, 1987) and in GA-deficient mutants of barley (Chandler, 1999). Increased abortion of seeds carrying the 35S:2ox2 transgene also can explain the low transformation efficiency observed for this construct using vacuum infiltration, because this method initially generates transgenic seeds that must successfully complete development before transgenic plants can be identified (Ye et al, 1999).…”

Section: Gas and Seed Developmentsupporting

confidence: 79%

See 1 more Smart Citation

Gibberellins Are Required for Seed Development and Pollen Tube Growth in Arabidopsis

2002

View full text Add to dashboard Cite

Gibberellins (GAs) are tetracyclic diterpenoids that are essential endogenous regulators of plant growth and development. GA levels within the plant are regulated by a homeostatic mechanism that includes changes in the expression of a family of GA-inactivating enzymes known as GA 2-oxidases. Ectopic expression of a pea GA 2-oxidase2 cDNA caused seed abortion in Arabidopsis, extending and confirming previous observations obtained with GA-deficient mutants of pea, suggesting that GAs have an essential role in seed development. A new physiological role for GAs in pollen tube growth in vivo also has been identified. The growth of pollen tubes carrying the 35S:2ox2 transgene was reduced relative to that of nontransgenic pollen, and this phenotype could be reversed partially by GA application in vitro or by combining with spy-5 , a mutation that increases GA response. Treatment of wild-type pollen tubes with an inhibitor of GA biosynthesis in vitro also suggested that GAs are required for normal pollen tube growth. These results extend the known physiological roles of GAs in Arabidopsis development and suggest that GAs are required for normal pollen tube growth, a physiological role for GAs that has not been established previously.

show abstract

Section: Gas and Seed Developmentsupporting

confidence: 79%

“…In tomato, Groot et al (1987) used the GAdeficient gib1 mutant to suggest that GAs are required for normal seed growth. In pea, two alleles at the LH locus, lh-1 and lh-2 , have been used to demonstrate that GAs produced in the embryo and/or endosperm are required for normal seed growth and survival (Swain et al, 1993(Swain et al, , 1997.…”

Section: Introductionmentioning

confidence: 99%

Gibberellins Are Required for Seed Development and Pollen Tube Growth in Arabidopsis

2002

View full text Add to dashboard Cite

show abstract

“…These observations suggest that GAs are involved in the early stage of seed development in pea (Swain et al, 1995). On the other hand, Groot et al (1987) have reported that exogenous GA increased the seed weight and delayed seed dehydration of the GAdeficient ga-1 mutant of tomato (Lycopersicon esculentum), which suggests that GAs played a role even in the later stages of fruit and seed development in the tomato. Scialabba et al (1999) have observed starch grains in the integument of developing seeds of Brassica macrocarpa and their gradual degradation and disappearance during the process of seed maturation.…”

mentioning

confidence: 90%

Immunohistochemistry of Active Gibberellins and Gibberellin-Inducible α-Amylase in Developing Seeds of Morning Glory

Nakayama

Park

et al. 2002

Plant Physiology

View full text Add to dashboard Cite

Gibberellins (GAs) in developing seeds of morning glory (Pharbitis nil) were quantified and localized by immunostaining. The starch grains began to be digested after the GA contents had increased and reached a plateau. Immunohistochemical staining with the antigibberellin A1-methyl ester-antiserum, which has high affinity to biologically active GAs, showed that GA1 and/or GA3 were localized around starch grains in the integument of developing young seeds, suggesting the participation of GA-inducible α-amylase in this digestion. We isolated an α-amylase cDNA (PnAmy1) that was expressed in the immature seeds, and using an antibody raised against recombinant protein, it was shown that PnAmy1 was expressed in the immature seeds. GA responsiveness of PnAmy1 was shown by treating the young fruits 9 d after anthesis with GA3. RNA-blot and immunoblot analyses showed that PnAmy1 emerged soon after the rapid increase of GA1/3. An immunohistochemical analysis of PnAmy1 showed that it, like the seed GA1/3, was also localized around starch grains in the integument of developing young seeds. The localization of GA1/3 in the integument coincident with the expression of PnAmy1 suggests that both function as part of a process to release sugars for translocation or for the further development of the seeds.

show abstract

“…Since GA, was not found as an endogenous GA in tomato, exogenous GA, could not have been as effective as endogenous GAs. Although tomato fruits may grow in the absence of endogenous GAs (ga-l mutant), they develop much better with GAs (Groot et al, 1987). Differences in GA-induced responses in tomato can be partially explained by timing of application (Sawhney, 1984).…”

Section: Fruit Development and Growth Regulatorsmentioning

confidence: 99%

Arginase, Arginine Decarboxylase, Ornithine Decarboxylase, and Polyamines in Tomato Ovaries (Changes in Unpollinated Ovaries and Parthenocarpic Fruits Induced by Auxin or Gibberellin)

et al. 1996

View full text Add to dashboard Cite

Arginase (EC 3.5.3.1) activity has been found in the ovaries and young fruits of tomato (Lycopersicon esculentum Mill. cv Rutgers). Changes in arginase, arginine decarboxylase (EC 4.1.1.1 9), and ornithine decarboxylase activity (EC 4.1 .I .17) and levels of free and conjugated putrescine, spermidine, and spermine were determined in unpollinated ovaries and in parthenocarpic fruits during the early stages of development induced by 2,4-dichlorophenoxyacetic acid (2,4-D) or gibberellic acid (CA,). Levels of arginase, free spermine, and conjugates of the three polyamines were constant in unpollinated ovaries and characteristic of a presenescent step. A marked decrease in arginase activity, free spermine, and polyamine conjugates was associated with the initiation of fruit growth due to cell division, and when cell expansion was initiated, the absence of arginase indicated a redirection of nitrogen metabolism to the synthesis of arginine. A transient increase in arginine decarboxylase and ornithine decarboxylase was also observed in 2,4-D-induced fruits. In general, 2,4-D treatments produced faster changes than CA,, and without treatment, unpollinated ovaries developed only slightly and senescence was hardly visible. Sensitivity to 2,4-D and CA, treatment remained for at least 2 weeks postanthesis.

show abstract

The role of endogenous gibberellin in seed and fruit development of tomato: Studies with a gibberellin‐deficient mutant

Cited by 95 publications

References 15 publications

Gibberellins Are Required for Seed Development and Pollen Tube Growth in Arabidopsis

Gibberellins Are Required for Seed Development and Pollen Tube Growth in Arabidopsis

Immunohistochemistry of Active Gibberellins and Gibberellin-Inducible α-Amylase in Developing Seeds of Morning Glory

Arginase, Arginine Decarboxylase, Ornithine Decarboxylase, and Polyamines in Tomato Ovaries (Changes in Unpollinated Ovaries and Parthenocarpic Fruits Induced by Auxin or Gibberellin)

Contact Info

Product

Resources

About