The role of formate metabolism in nitrogen fixation in Rhizobium Spp.

Manian, Sundaram S.; Gumbleton, Robert; O’Gara, Fergal

doi:10.1007/bf00521297

Cited by 19 publications

(11 citation statements)

References 19 publications

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“…Formate dehydrogenase, delta-subunit (FdhD) is involved in the metabolism of formate into CO 2 , formate-dependent nitrogen fixation in Rhizobium spp. (Manian et al 1982), assimilation of formaldehyde in a photosynthetic bacterium (Barber and Donohue 1998) and C1 metabolism that is inter-connected with benzoate metabolism (Denef et al 2004 and 2006). Rapid transformation of carbaryl to one carbon unit metabolites ( e.g.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic and proteomic insights into carbaryl catabolism by Burkholderia sp. C3 and degradation of ten N-methylcarbamates

Seo

Keum

2013

Biodegradation

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Burkholderia sp. C3, an efficient polycyclic aromatic hydrocarbon (PAH) degrader, can utilize 9 of the 10 N-methylcarbamate insecticides including carbaryl as a sole source of carbon. Rapid hydrolysis of carbaryl in C3 is followed by slow catabolism of the resulting 1-naphthol. This study focused on metabolomes and proteomes in C3 cells utilizing carbaryl in comparison to those using glucose or nutrient broth. Sixty of the 867 detected proteins were involved in primary metabolism, adaptive sensing and regulation, transport, stress response, and detoxification. Among the 41 proteins expressed in response to carbaryl were formate dehydrogenase, aldehyde-alcohol dehydrogenase and ethanolamine utilization protein involved in one carbon metabolism. Acetate kinase and phasin were 2 of the 19 proteins that were not detected in carbaryl-supported C3 cells, but detected in glucose-supported C3 cells. Down-production of phasin and polyhydroxyalkanoates in carbaryl-supported C3 cells suggests insufficient carbon sources and lower levels of primary metabolites to maintain an ordinary level of metabolism. Differential metabolomes (approximately 196 identified polar metabolites) showed up-production of metabolites in pentose phosphate pathways and metabolisms of cysteine, cystine and some other amino acids, disaccharides and nicotinate, in contract to down-production of most of the other amino acids and hexoses. The proteomic and metabolomic analyses showed that carbaryl-supported C3 cells experienced strong toxic effects, oxidative stresses, DNA/RNA damages and carbon nutrient deficiency.

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Section: Discussionmentioning

confidence: 99%

Metabolomic and proteomic insights into carbaryl catabolism by Burkholderia sp. C3 and degradation of ten N-methylcarbamates

Seo

Keum

2013

Biodegradation

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“…Unlike E. coli, however, the involvement of cAMP in catabolite repression ~ of enzyme synthesis in Rhizobium is not firmly established. In the present study, malate was chosen to exert catabolite re, pression in Rhizobium [16,18]. Uninduced /3galactosidase levels on malate were 22.0and 1.7 nmol/min/A6oon m for R. meliloti and R. japonicurn, respectively ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Expression and regulation of the lactose transposon Tn951 in Rhizobium spp.

McGetrick¹

1985

FEMS Microbiology Letters

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The expression of β‐galactosidase by the lac transposon Tn951, in Escherichia coli, was found to be cAMP‐dependent. This finding provided the basis for an investigation of the effect of cAMP on Tn951 lac expression in Rhizobium, with the ultimate aim of using the Tn951 system as a specific probe for cAMP mediated catabolite repression. When introduced into Rhizobium, Tn951 directed the synthesis of β‐galactosidase, which was inducible by isopropyl‐β‐d‐thiogalactopyranoside (IPTG). Marked quantitative and qualitative differences in β‐galactosidase expression were found between R. meliloti and R. japonicum during the growth cycle, with expression being higher in the former. β‐Galactosidase levels were, however, unaffected by exogenous cAMP under catabolite repressing conditions.

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“…The mutagen used was mitomycin C (25 pLg/ml; obtained from Sigma London Chemical Company, Poole, Dorset, England), and the mutagenesis procedure used was described previously (12). Clones that could not grow autotrophically on the mineral medium and were white on formate-tetrazolium plates (12) were selected. These clones were unable to grow in formate minimal medium.…”

Section: Methodsmentioning

confidence: 99%

Nitrogen Fixation and Carbon Dioxide Assimilation in Rhizobium japonicum

Manian

Gumbleton

Buckley

et al. 1984

Appl Environ Microbiol

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In free-living Rhizobium japonicum cultures, the stimulatory effect of CO2 on nitrogenase (acetylene reduction) activity was mediated through ribulose bisphosphate carboxylase activity. Two mutant strains (CJ5 and CJ6) of R. japonicum defective in CO2 fixation were isolated by mitomycin C treatment. No ribulose bisphosphate carboxylase activity could be detected in strain CJ6, but a low level of enzyme activity was present in strain CJ5. Mutant strain CJ5 also exhibited pleiotropic effects on carbon metabolism. The mutant strains possessed reduced levels of hydrogen uptake, formate dehydrogenase, and phosphoribulokinase activities, which indicated a regulatory relationship between these enzymes. The C02-dependent stimulation of nitrogenase activity was not observed in the mutant strains. Both mutant strains nodulated soybean plants and fixed nitrogen at rates comparable to that of the wild-type strain.

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The role of formate metabolism in nitrogen fixation in Rhizobium Spp.

Cited by 19 publications

References 19 publications

Metabolomic and proteomic insights into carbaryl catabolism by Burkholderia sp. C3 and degradation of ten N-methylcarbamates

Metabolomic and proteomic insights into carbaryl catabolism by Burkholderia sp. C3 and degradation of ten N-methylcarbamates

Expression and regulation of the lactose transposon Tn951 in Rhizobium spp.

Nitrogen Fixation and Carbon Dioxide Assimilation in Rhizobium japonicum

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