The role of heat shock protein (HSP ) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture

Mulyani, Sri Wigati Mardi; Setiawati, Ernie Maduratna; Safitri, Erma; Astuti, Eha Renwi

doi:10.20473/j.djmkg.v47.i1.p41-44

Cited by 5 publications

(14 citation statements)

References 8 publications

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“…MSC characterization between low O 2 and normoxic culture conditions was performed before being transferred into the ovary based on morphological analysis of cells using an inverted microscope (Phase Contrast, MXD-400, Nanjing BW Optics & Instrument Co., Ltd). HSP70 and caspase- 15 Real-time PCR was performed using the following method. The MSC-containing media were aspirated, rinsed two times with 1 mL of PBS, and then added with 500 μL of RNAiso/well.…”

Section: Msc Characterizationmentioning

confidence: 99%

“…Recently, researchers cultivating stem cells under low oxygen tension have observed cell stress, although one study has found that the stress situation activates HSP27 as a function of anti-apoptosis via caspase-9 suppression. 15 The success of stem cell transplantations is limited by their weak adaptivity and differentiation. The low efficacy of MSC transplantation might be due to apoptosis occurring during cell growth.…”

Section: Introductionmentioning

confidence: 99%

“…The low efficacy of MSC transplantation might be due to apoptosis occurring during cell growth. 15,16 Consequently, high doses of stem cells via regular boosters are necessary for effective therapy, which increases the therapy cost. 17 In vitro stem cell cultivation must be adapted to a niche environment, such as the microenvironment of bone marrow, to avoid apoptosis.…”

Section: Introductionmentioning

confidence: 99%

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Role of apoptotic inhibitors, viability, and differentiation in low oxygen tension of mesenchymal stem cells cultured in a rat model of ovarian failure

et al. 2023

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Background: Stem cell therapy shows applications potential for malnutrition-induced ovarian failure in rat models. However, it is ineffective because of the lack of viability and differentiation of transplanted stem cells, resulting in low adaptation and survival rates. We aimed to determine whether stem cells cultured under low oxygen (O2) tension improves the adaptability and viability of stem cells, as well as ovarian failure. Methods: After four days of culturing mesenchymal stem cells (MSCs) in 21% oxygen (normoxia) as the T2 group and 1% oxygen (low O2 or hypoxia) as the T1 group, 200 million bone marrow-derived MSCs per rat were transplanted into female rats with ovarian failure (15 rats per treatment group). A total of 15 fertile and 15 infertile rats were categorized as the C+ and C− groups, respectively. Results: The slight increase in cells expressing HSP70 (C+, T2, T1, and C− groups were 0.5a±0.53, 1.7a±0.82, 6.2b±1.5, and 9.6c±1.3, respectively), decrease in cells expressing caspase-3 as an apoptotic inhibitor (C+, T2, T1, and C− groups were 0.2a±0.42, 0.6a±0.52, 4.8b±1.03, and 7.3c±1.42, respectively), and increase in cells expressing VEGF-1 (C+, T2, T1, and C− groups were 10.8c±1.55, 8.7b±0.48, 0.4a±0.52, and 0.2a±0.42, respectively) and GDF-9 (C+, T2, T1, and C− groups were 5.8c±1.47, 4.6b±0.97, 0.5a±0.53, and 0.3a±0.48, respectively) were used as markers for viability and differentiation in ovarian tissue, indicating that MSCs cultured under low O2 tension were more effective than those cultured under normoxic conditions as a treatment for female rats with ovarian failure. Furthermore, infertile female rats treated with MSCs cultivated under low O2 tension had an enhanced ovarian tissue shape, as indicated by the increasing Graafian follicle count (C+, T2, T1, and C− groups were 8.9c±0.74, 4.5b±0.71, 0.5a±0.53, and 0.4a±0.52, respectively). Conclusions: MSCs cultured under low O2 tension are an effective treatment for malnourished rats with ovarian failure.

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Section: Msc Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of apoptotic inhibitors, viability, and differentiation in low oxygen tension of mesenchymal stem cells cultured in a rat model of ovarian failure

et al. 2023

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“…The reduced viability of MSCs is thought to be caused by a normoxia culture with a high oxygen concentration (O 2 >20%). Cell senescence [ 7 ], cell apoptosis [ 8 ], and gene mutation [ 9 ] can all be caused by normoxia culture. As a result, the low viability of MSCs restricts the success of cell transplant therapy.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)

Safitri

Purnobasuki

2021

Vet World

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Background and Aim: Mesenchymal stem cells (MSCs) transplanted into the testes of rats with testicular failure can help rescue fertility. However, the low viability of transplanted MSCs limits the success of this treatment. This study aimed to determine the effectiveness of MSCs cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus). Materials and Methods: Bone marrow-derived MSCs (200 million cells/rat) were transplanted into male rat models with induced infertility (10 rats/treatment group) after 4 days of culture in 21% O2 (normoxia) and 1% O2 (hypoxia). Ten fertile and 10 untreated infertile rats served as controls. In the infertile male rats that had been fasted from food for 5 days, the fasting condition induced malnutrition and then resulted in testicular failure. Results: The results indicated that the MSCs cultured under hypoxic conditions were more effective than those cultured in normoxic conditions as a treatment for testicular failure in infertile male rats based on the increased number of cells expressing p63 as a quiescent cell marker and ETV5 as a transcription factor expressed in Sertoli and germ cells. Furthermore, the structure of the seminiferous tubules, which contain spermatogonia, primary and secondary spermatocytes, and spermatid, Sertoli, and Leydig cells, was improved in infertile male rats treated with the MSCs cultured under hypoxic conditions. Conclusion: The testicular transplantation of MSCs cultured under hypoxic conditions was an effective treatment for testicular failure in rats.

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“…The efficacy of the therapy was limited due to low viability of stem cells after transplantation [6,7], which is due to the conventional in vitro culture of normoxia-conditioned rat MSCs (rMSC-CN) with oxygen concentration of >21%. The conventional culture causes cell senescence [8], apoptosis [9], and gene mutation, such as G: C to T: A [10]. Some researchers showed that >93% of stem cells perish between 1 and 7 days after transplantation [11-15].…”

Section: Introductionmentioning

confidence: 99%

Comparison of biotechnological culture of hypoxia-conditioned rat mesenchymal stem cells with conventional in vitro culture of normoxia-conditioned rat mesenchymal stem cells for testicular failure therapy with low libido in rats

Safitri

Hariadi

2019

Vet World

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Aim: Biotechnological culture of hypoxia-conditioned (CH) rat mesenchymal stem cells (rMSC-CH) for testicular failure therapy with low libido improves the functional outcome of the testicle for producing spermatogenic cells and repairs Leydig cells in rats (Rattus norvegicus). Materials and Methods: In the first group (T1), rats with testicular failure and low libido were injected with normoxia-conditioned (CN) rMSCs (21% oxygen); in the second group (T2), rats with testicular failure and low libido were injected with rMSC-CH (1% oxygen); in the negative control group (T–), rats with normal testis were injected with 0.1 mL phosphate-buffered saline (PBS); and in the sham group (TS), rats with testicular failure and low libido were injected with 0.1 mL of PBS. Results: Vascular endothelial growth factor expression, as the homing signal, in the groups T2, T–, T1, and TS was 2.00±0.5%, 2.95±0.4%, 0.33±0.48%, and 0±0%, respectively. The number of cluster of differentiation (CD)34+ and CD45+ cells in the groups T– and TS was <20%, whereas that in T1 and T2 groups was >30% and >80%, respectively, showing the mobilization of hematopoietic stem cells (HSCs). The number of spermatogenic cells (spermatogonia, primary spermatocytes, secondary spermatocytes, and spermatid) decreased significantly (p<0.05) in TS compared with that in T–, T1, and T2, whereas that in T2 did not show a significant (p>0.05) decrease compared to that in T–. The improvement in libido, based on the number of Leydig cells producing the hormone testosterone for libido expression, did not increase in T1, whereas T2 was able to maintain the number of Leydig cells significantly compared to that between TS and T1. Conclusion: rMSC-CH culture for testicular failure with low libido showed improvement in the functional outcome of the testicle and in repairing Leydig cells.

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The role of heat shock protein (HSP ) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture

Cited by 5 publications

References 8 publications

Role of apoptotic inhibitors, viability, and differentiation in low oxygen tension of mesenchymal stem cells cultured in a rat model of ovarian failure

Role of apoptotic inhibitors, viability, and differentiation in low oxygen tension of mesenchymal stem cells cultured in a rat model of ovarian failure

Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)

Comparison of biotechnological culture of hypoxia-conditioned rat mesenchymal stem cells with conventional in vitro culture of normoxia-conditioned rat mesenchymal stem cells for testicular failure therapy with low libido in rats

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