The Role of Heavy Metals-Resistant Bacteria Acinetobacter sp. in Copper Phytoremediation using Eichhornia crasippes [(Mart.) Solms]

Irawati, Wahyu; Parhusip, Adolf J.N.; Sopiah, Nida; Tnunay, Juniche Anggelique

doi:10.18502/kls.v3i5.995

Cited by 14 publications

(7 citation statements)

References 27 publications

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“…This rise in pH might occurred be due to the consumption of carbon dioxide by the plants during the process of photosynthesis. Irawati et al 62 has already reported that an increase in pH might be accredited to the consumption of CO 2 during the process of photosynthesis by macrophytes. Similar results regarding the rise in pH have been reported by Galal et al 63 , Polechońska et al 64 and Zhao et al 65 in their research studies carried out on free floating aquatic macrophytes for different wastewater streams.…”

Section: Resultsmentioning

confidence: 98%

Domestic wastewater treatment by Pistia stratiotes in constructed wetland

Ali,

Aslam,

Qadeer

et al. 2024

Sci Rep

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The objective of the study was to evaluate the performance of Pistia stratiotes for treatment of domestic wastewater in a free surface water flow constructed wetland. The objective of the study was to evaluate contaminants removal efficiency of the constructed wetland vegetated with P. stratiotes in treatment of domestic wastewater against Hydraulic retention time (HRT) of 10, 20 and 30 days was investigated. This asks for newer and efficient low-cost nature-based water treatment system which along with cost takes into consideration the sustainability of the ecosystem. Five constructed wetland setups improved the wastewater quality and purify it significantly by reducing the TDS by 83%, TSS by 82%, BOD by 82%, COD by 81%, Chloride by 80%, Sulfate by 77%, NH3 by 84% and Total Oil and Grease by 74%. There was an increase in pH of about 11.9%. Color and odor of wastewater was also improved significantly and effectively. It was observed that 30 days’ HRT was optimum for the treatment of domestic wastewater. The final effluent was found to be suitable as per national environmental quality standards and recycled for watering plants and crop irrigation but not for drinking purposes. The treatment in constructed wetland system was found to be economical, as the cost of construction only was involved and operational and maintenance cost very minimal. Even this research was conducted on the sole purpose of commuting the efficiency of pollutant removal in short span time.

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Section: Resultsmentioning

confidence: 98%

Domestic wastewater treatment by Pistia stratiotes in constructed wetland

Ali,

Aslam,

Qadeer

et al. 2024

Sci Rep

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“…Eichhornia presented high microbial diversity and richness in the rhizosphere. Studies have showed that Eichhornia, like other aquatic macrophytes, has a specialized rhizosphere microbiota capable of resisting and removing heavy metals and other water pollutants as well (Abou-Shanab et al, 2007;El-Deeb et al, 2012;Sharma et al, 2013;Kabeer et al, 2014;Irawati et al, 2017). The Eichhornia rhizosphere community presented the dominance of an unknown bacterial genus of Moraxellaceae family (17.8%), that was also observed on water samples but, interestingly, only on samples collected one year later of disaster.…”

Section: Discussionmentioning

confidence: 95%

Adaption of microbial communities to the hostile environment in the Doce River after the collapse of two iron ore tailing dams

Giongo

Borges

Marconatto

et al. 2020

Heliyon

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In November 2015, two iron ore tailing dams collapsed in the city of Mariana, Brazil. The dams' collapse generated a wave of approximately 50 million m 3 of a mixture of mining waste and water. It was a major environmental tragedy in Brazilian history, which damaged rivers, and cities 660 km away in the Doce River basin until it reached the ocean coast. Shortly after the incident, several reports informed that the concentration of metals in the water was above acceptable legal limits under Brazilian laws. Here the microbial communities in samples of water, mud, foam, and rhizosphere of Eichhornia from Doce River were analyzed for 16S and 18S rRNA-based amplicon sequencing, along with microbial isolation, chemical and mineralogical analyses. Samples were collected one month and thirteen months after the collapse. Prokaryotic communities from mud shifted drastically over time (33% Bray-Curtis similarity), while water samples were more similar (63% Bray-Curtis similarity) in the same period. After 12 months, mud samples remained with high levels of heavy metals and a reduction in the diversity of microeukaryotes was detected. Amoebozoans increased in mud samples, reaching 49% of microeukaryote abundance, with Discosea and Lobosa groups being the most abundant. The microbial communities’ structure in mud samples changed adapting to the new environment condition. The characterization of microbial communities and metal-tolerant organisms from such impacted environments is essential for understanding the ecological consequences of massive anthropogenic impacts and strategies for the restoration of contaminated sites such as the Doce River.

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“…Furthermore, copper toxicity may block functional groups on the cell surface, the substitution of essential metal ions through biomolecular modifications, denaturation, and inactivation of enzymes, along with the disruption of cellular and organellar membrane integrity (Gadd, 1993). Another possibility is that copper-binding sites on the bacterial cell membrane lack both the availability and capacity to accumulate copper at higher concentrations, most likely due to exhaustion from an extended overexposure to copper (Irawati et al, 2017b). Timkova et al (2018) defined bioaccumulation as the absorption of toxic pollutants that enter actively into the cell across the cell membrane and accumulate intracellularly.…”

Section: Impact Of Copper On the Growth Of Acinetobacter Sp Irc2 At Different Concentrationsmentioning

confidence: 99%

Optimizing Bioremediation: Elucidating Copper Accumulation Mechanisms of Acinetobacter sp. IrC2 Isolated From an Industrial Waste Treatment Center

et al. 2021

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Acinetobacter sp. IrC2 is a copper-resistant bacterium isolated from an industrial waste treatment center in Rungkut, Surabaya. Copper-resistant bacteria are known to accumulate copper inside the cells as a mechanism to adapt to a copper-contaminated environment. Periplasmic and membrane proteins CopA and CopB have been known to incorporate copper as a mechanism of copper resistance. In the present study, protein profile changes in Acinetobacter sp. IrC2 following exposure to copper stress were analyzed to elucidate the copper resistance mechanism. Bacteria were grown in a Luria Bertani agar medium with and without CuSO4 supplementation. Intracellular copper ion accumulation was quantified using atomic absorption spectrophotometry. Changes in protein profile were assessed using sodium dodecyl sulfate polyacrylamide gel electrophoresis. The results showed that 6 mM CuSO4 was toxic for Acinetobacter sp. IrC2, and as a response to this copper-stress condition, the lag phase was prolonged to 18 h. It was also found that the bacteria accumulated copper to a level of 508.01 mg/g of cells’ dry weight, marked by a change in colony color to green. The protein profile under copper stress was altered as evidenced by the appearance of five specific protein bands with molecular weights of 68.0, 60.5, 38.5, 24.0, and 20.5 kDa, suggesting the presence of CopA, multicopper oxidase (MCO), CopB, universal stress protein (Usp), and superoxide dismutase (SOD) and/or DNA-binding protein from starved cells, respectively. We proposed that the mechanism of bacterial resistance to copper involves CopA and CopB membrane proteins in binding Cu ions in the periplasm and excreting excess Cu ions as well as involving enzymes that play a role in the detoxification process, namely, SOD, MCO, and Usp to avoid cell damage under copper stress.

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The Role of Heavy Metals-Resistant Bacteria Acinetobacter sp. in Copper Phytoremediation using Eichhornia crasippes [(Mart.) Solms]

Cited by 14 publications

References 27 publications

Domestic wastewater treatment by Pistia stratiotes in constructed wetland

Domestic wastewater treatment by Pistia stratiotes in constructed wetland

Adaption of microbial communities to the hostile environment in the Doce River after the collapse of two iron ore tailing dams

Optimizing Bioremediation: Elucidating Copper Accumulation Mechanisms of Acinetobacter sp. IrC2 Isolated From an Industrial Waste Treatment Center

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