The role of IFITM3 in the growth and migration of human glioma cells

Abstract

BackgroundInterferon induced transmembrane protein 3 (IFITM3) is transcribed in most tissues and highly interferon-inducible. However, the role of IFITM3 in cancer is still poorly understood.MethodsExpression levels ofIFITM3were analyzed in 60 glioma patients by immunohistochemistry (IHC). Following closely, we investigated the phenotype of IFITM3 knockdown on glioma cell growth and tumorigenesis in vitro using lentivirus-mediated loss-of-function strategy.ResultsDepletion of IFITM3in U251 cells dramatically inhibited cell proliferation and colony formation, which demonstrated that reduced IFITM3 protein levels could cause inhibition of tumorigenesis. Knockdown of IFITM3 also induced cell cycle arrest in G0/G1 phase, especially in the sub-G1 phase representing apoptotic cells. In addition, the migration of U251 cells was visibly weakened after IFITM3 knockdown, as determined by Transwell assay.ConclusionsOur findings provide new evidence that IFITM3 plays an important role in glioma cell growth and migration, suggesting that silencing of IFITM3 by RNA interference (RNAi) may be a potential approach to suppress glioma growth.

The role of FoxJ2 in the migration of human glioma cells

“…To date, the underlying molecular mechanisms that determine the biologic behavior of glioma remain poorly understood. The identification of useful markers for the diagnosis of human glioma is a major goal in cancer research, which also provides valuable information in tumor pathogenesis [20]. The present study is the first to report that FoxJ2 was down-regulated in glioma, and it may suppress cell mobility in the development of glioma cells.…”

Overexpression of IFITM3 predicts poor prognosis in stage IIA esophageal squamous cell carcinoma after Ivor Lewis esophagectomy

“…IFITM3, also known as 1‐8U, is an important member of the interferon (IFN)‐inducible transmembrane protein family . It is reported to be upregulated and is associated with carcinogenesis and progression in in many human malignancies, such as gastric and breast cancers, colorectal tumors, and gliomas . IFITM3 likely exerts a profound influence on cell proliferation, migration, and invasion by modulating the Wnt/β‐catenin signaling pathway and is implicated in the G0/G1 checkpoint to control the cell cycle of tumors .…”

Transcriptome sequencing of neurologic diseases associated genes in HHV-6A infected human astrocyte

“…Elevated expression of chitinase 3-like 1 (CHI3L1, YKL-40) in glioma was correlated with decreases in disease survival [67, 68]. YKL-40 serum values were markedly higher in glioma patients than in healthy subjects [69], and as potential serum biomarker for patients with high-grade glioma [70]. TNF alpha induced protein 3 (TNFAIP3, A20) is a tumor enhancer in glioma [71] and inhibits apoptosis in glioblastoma [72].…”