The role of IGF1 in the in vivo production of bovine embryos from superovulated donors

Velazquez, Miguel A.; Zaraza, J.; Oropeza, A.; Webb, Robert I.; Niemann, Heiner

doi:10.1530/rep-08-0362

Cited by 74 publications

(47 citation statements)

References 257 publications

(157 reference statements)

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“…This is in agreement with Velazquez et al (24), who stated that higher doses of IGF-1 induce downregulation of IGF-1 receptors.…”

Section: Discussionsupporting

confidence: 93%

Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability

panahi¹,

Tajik²,

Movahedin³

et al. 2014

Turk J Vet Anim Sci

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In this survey, 5 male Holstein Friesian calves of 3 months old were used. The research was conducted in accordance with National Research Council guidelines. Calves were sedated with xylazine (0.1 mg/kg of body weight, i.m.) and received a 3-mL injection of 2% lidocaine 20 min before surgery. Respiration rate, heart rate, and rectal temperature were monitored before and after recovery from analgesia. Calves were given flunixin meglumine (2.2 mg/kg of body weight, i.m.) for 3 days after surgery.

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“…This is in agreement with Velazquez et al (24), who stated that higher doses of IGF-1 induce downregulation of IGF-1 receptors.…”

Section: Discussionsupporting

confidence: 93%

Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability

panahi¹,

Tajik²,

Movahedin³

et al. 2014

Turk J Vet Anim Sci

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“…In contrast, in a naturally occurring high IGF1 microenvironment such as with PCOS (Thierry van Dessel et al 1999), the embryos are continuously exposed to abnormally high levels of IGF1, which may exacerbate the phenotype observed in our in vitro model (high apoptosis and hypertrophic ICM). Furthermore, given that oestrogens regulate to a great extent IGF1 production in the uterus (Velazquez et al 2009), an increased paracrine action of IGF1 caused by the greater bioavailability of free oestrogens present in the endometrium of PCOS women (Leon et al 2008) would lead to an impaired endometrial function (Lathi et al 2002, Giudice 2006) that would further reduce chances of normal pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Increased apoptosis in bovine blastocysts exposed to high levels of IGF1 is not associated with downregulation of the IGF1 receptor

Velazquez¹,

Hermann²,

Kues³

et al. 2011

Reproduction

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The hypothesis that high concentrations of IGF1 can impair embryo development was investigated in a bovine in vitro model to reflect conditions in polycystic ovary syndrome (PCOS) patients. Embryos were either cultured in the absence or presence of a physiological (100 ng/ml) or supraphysiological (1000 ng/ml) IGF1 concentration. Cell allocation, apoptosis, transcript and protein expression of selected genes involved in apoptosis, glucose metabolism and the IGF system were analysed. Supraphysiological IGF1 concentration did not improve blastocyst formation over controls, but induced higher levels of apoptosis, decreased TP53 protein expression in the trophectoderm and increased the number of cells in the inner cell mass (ICM). The increase in ICM cells corresponded with an increase in IGF1 receptor (IGF1R) protein in the ICM. A small, but significant, percentage of blastocysts displayed a hypertrophic ICM, not observed in controls and virtually absent in embryos treated with physiological concentrations of IGF1. Physiological IGF1 concentrations increased total IGF1R protein expression and upregulated IGFBP3 transcripts leading to an increase in blastocyst formation with no effects on cell number or apoptosis. In conclusion, the results support the hypothesis of detrimental effects of supraphysiological IGF1 concentrations on early pregnancy. However, our results do not support the premise that increased apoptosis associated with high levels of IGF1 is mediated via downregulation of the IGF1R as previously found in preimplantation mouse embryos. This in vitro system with the bovine preimplantation embryo reflects critical features of fertility in PCOS patients and could thus serve as a useful model for in-depth mechanistic studies.

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“…Insulin bind to cell surface receptor and its action is receptor-mediated. It has been demonstrated that insulin stimulates glucose and amino acid uptake and protein synthesis (Harvey and Kaye, 1988;Kane et al, 1997) of mouse embryos (Velazquez et al, 2009;Kaye and Harveyt, 1995). Several studies have indicated that insulin increases the in vitro oocyte maturation and development of human (Dashtizad et al, 2003), mice (Demeestere et al, 2004) and porcine embryos .…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Insulin on <i>in vitro</i> Development of Immature Bovine Oocytes

Dashtizad¹,

Wahid²,

Yusoff³

et al. 2010

American Journal of Animal and Veterinary Sciences

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Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes and in vitro fertilized embryos. Approach: Grade one cumulus-oocyte-complexes harvested from slaughterhouse ovaries were selected and randomly allocated in each treatment groups. In experiment 1, in vitro maturation medium (Hepes-buffered medium 199 + fetal calf serum + gonadotrophins + antibiotics) supplemented with 0 (control), 1, 10, 20 and 100 µg mL −1 of insulin. In experiment 2, to eliminate the effect of serum and hormones, Hepesbuffered medium 199 was supplemented with 1 mg mL −1 polyvinyl alcohols (PVA) and same levels of insulin. In experiment 3, the effect of insulin on bovine in vitro embryo development was assessed. Presumptive zygotes were randomly cultured in synthetic oviductal fluid added with 0 (control), 1, 10, 20 and 100 µg mL −1 of insulin. Results: In experiment 1, nuclear maturation and embryo development rates were significantly higher in 1 and 10 µg mL −1 compared with other groups (P<0.05). In experiment 2, both maturation and cleavage rate significantly increased in 1 and 10 µg mL −1 insulin.The only treatment resulted in higher hatchability was 10 µg mL −1 insulin (17.1±2.34%) compared with control (11.34±3.94). In experiment 3, cleavage and morula rates were significantly greater in 1 and 10 µg mL −1 insulin compared with other groups; although the highest rates resulted by using 10 µg mL −1 . Conclusion: Obtained results show that inclusion of 10 µg mL −1 insulin in maturation and culture medium exerted beneficial effects on nuclear maturation of bovine oocytes and in vitro embryo development till morula stage.

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The role of IGF1 in the in vivo production of bovine embryos from superovulated donors

Cited by 74 publications

References 257 publications

Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability

Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability

Increased apoptosis in bovine blastocysts exposed to high levels of IGF1 is not associated with downregulation of the IGF1 receptor

Synergistic Effect of Insulin on <i>in vitro</i> Development of Immature Bovine Oocytes

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