The role of inflammatory miRNA–mRNA interactions in PBMCs of colorectal cancer and obesity patients

Abstract

IntroductionInflammation is a critical hallmark in obesity and colorectal cancer (CRC). This study aimed to investigate effective microRNA (miRNA)–messenger RNA (mRNA) interactions on inflammatory networks involved in obesity and CRC.MethodsThe literature searches were applied to identify genes expression reported on peripheral blood mononuclear cells (PBMCs) and/or blood of CRC subjects and to find inflammatory miRNA in blood samples. Furthermore, bioinformatics analysis was utilized to find inflammatory miRNA:mRNA interactions of the genes. Finally, a case‐control study was set to investigate the expression of LAMC1 and GNB3 genes besides miR‐10b, miR‐506‐3p, miR‐150‐5p, and miR‐124‐3p in CRC and control subjects.ResultsThe expression of LAMC1 gene in healthy control groups was associated with body mass index (BMI) (p < .05). The level of miR‐10b (p < .001), miR‐506 (p < .001), and miR‐124 (p <. 001) were significantly increased in PBMCs of CRC patients, while they were not associated with BMI. The level of miR‐150 was associated with BMI in healthy subjects (p < .05).ConclusionsThe changes in the level of miR‐506 and miR‐124 in CRC patients may be associated with the regulatory role of these miRNAs on LAMC1 expression. The LAMC1 may be related to BMI, however, more observational studies on other populations are needed.

miR-124 as a Liquid Biopsy Prognostic Biomarker in Small Extracellular Vesicles from NSCLC Patients

“…This study is the first to identify miR-124 as a liquid biopsy biomarker of poor prognosis in NSCLC without driver mutations, regardless of stage. While the potential role of miR-124 as a tumor suppressor miRNA in cancer has been previously shown in various tumor types such as lung, colorectal cancer, brain tumors, osteosarcoma, and breast cancer [107][108][109][110][111][112], none of them have established a correlation between elevated levels of this miRNA in circulation, free, or exosomal forms and worse prognosis in these pathologies. Our study highlights the potential specificity of miR-124 to lung cancer based on the current results and our previous screening, in which miR-124 showed altered levels specifically in lung cancer cell lines [6].…”

Nutritional and functional status as indices of short- and long-term prognosis in patients undergoing surgery due to colorectal cancer

“…On the other, there is strong evidence that both malnutrition [10][11][12][13][14] and excess body fat, visceral adiposity, and sarcopenia (reduced skeletal muscle mass, strength and functional performance), especially when it coexists with obesity (sarcopenic obesity), contribute to worse short-, medium-and long-term outcomes of CRC treatment [1, 2, 6-8, 11-13, 15, 16]. Numerous explanations for associations between CRC surgery outcomes and patients' baseline nutritional status are proposed, including (a) the effect of adipocytokines (hormonal substances secreted by adipocytes and that induce insulin and insulin-like growth factor-1 [IGF-1] secretion), changes in appetite and energy expenditure, promotion of colonic www.journals.viamedica.pl/medical_research_journal cell proliferation, immune response, and angiogenesis [8,[17][18][19][20][21][22]; (b) possibility to perform of radical operation [1,2,4]; (c) the risk of perioperative complications [1,2]; (d) the response to chemotherapy [23] and the risk of chemotherapy-associated hepatotoxicity due to liver steatosis [4]; and (e) the risk of post-surgery neoplasmatic cachexia and frailty [14,24].…”