The role of katanin p60 in breast cancer bone metastasis

Abstract

p60 is a subunit of katanin involved in microtubule-severing. Previous studies of p60 were primarily focused on microtubule regulation and cell cycle regulation. More recent research has demonstrated that katanin p60 possesses a function in prostate cancer bone metastasis; however, its role in breast cancer bone metastasis remains unclear. In the present study, immunohistochemistry was used to analyze the expression of katanin p60 in primary and bone metastatic breast cancer. The role of up- and downregulated katanin p60 was investigated using cell proliferation, and migration experiments. Overall, katanin p60 was highly expressed in breast cancer bone metastatic tissue compared with primary tumor tissue. In breast cancer cells, overexpression of katanin p60 inhibited cell proliferation, but promoted cell migration, whereas silencing katanin p60 expression promoted cell proliferation but inhibited cell migration. Overall, the present study indicated that katanin p60 serves a role in cell proliferation and migration, and thus may be a novel therapeutic target for prevention of breast cancer metastasis.

Katanin P80 correlates with larger tumor size, lymph node metastasis, and advanced TNM stage and predicts poor prognosis in non–small‐cell lung cancer patients

“…Katanin consists of p60 and p80 subunits, and katanin P60 is responsible for microtubule‐severing activity directly via phosphorylation, while this activity is enhanced by the katanin P80 . Previous studies have revealed the role of katanin P60 in the initiation and development of several cancers . For example, one study illustrates that katanin P60 is highly expressed in breast cancer bone metastatic tissues compared with primary tumor tissues, and its overexpression promotes cell migration but inhibits cell proliferation in breast cancer, suggesting the important role of katanin P60 in breast cancer metastasis .…”

“…Previous studies have revealed the role of katanin P60 in the initiation and development of several cancers . For example, one study illustrates that katanin P60 is highly expressed in breast cancer bone metastatic tissues compared with primary tumor tissues, and its overexpression promotes cell migration but inhibits cell proliferation in breast cancer, suggesting the important role of katanin P60 in breast cancer metastasis . Another study investigates the association of katanin P60 with clinicopathological features and survival profile in breast cancer patients, which demonstrates that katanin P60 positive expression is correlated with higher lymph node metastasis, and its high expression independently predicted worse prognosis in breast cancer patients .…”

“…[17][18][19] For example, one study illustrates that katanin P60 is highly expressed in breast cancer bone metastatic tissues compared with primary tumor tissues, and its overexpression promotes cell migration but inhibits cell proliferation in breast cancer, suggesting the important role of katanin P60 in breast cancer metastasis. 17 Another study investigates the association of katanin P60 with clinicopathological features and survival profile in breast cancer patients, which demonstrates that katanin P60 positive expression is correlated with higher lymph node metastasis, and its high expression independently predicted worse prognosis in breast cancer patients. 18 In addition, katanin P60 is aberrantly expressed during the prostate cancer progression, and its overexpression is responsible for cancer cell metastasis via promoting cell motility in prostate cancer.…”

Katanin P60: a potential biomarker for lymph node metastasis and prognosis for non-small cell lung cancer

“…In cervical cancer, katanin P60 causes G 2 /M arrest, affects cell mitosis and tumor metastasis [20]. Katanin P60 is highly expressed in breast cancer bone metastasis tissue compared with primary tumor tissue, and overexpression of katanin P60 promotes cell migration but downregulation of katanin P60 suppresses cell migration in breast cancer cells [5]. In addition, katanin P60-high expression is reported to be correlated with LYN metastasis and poor OS in breast cancer patients [11].…”

“…Katanin, belonging to the family of microtubule severing enzymes, is a heterodimer with AAA catalytic subunit katanin P60 and non-AAA subunit katanin P80 [4]. Previous studies mostly focus on the role of katanin in mitosis, meiosis, and microtubule release from centrosome in the neuron, whereas only a few of them investigate the implication of katanin in malignancies [5][6][7][8]. One existing study indicates that katanin P80 correlates with larger tumor size, lymph node (LYN) metastasis, and advanced TNM stage and serves as a potential biomarker for predicting poor survival in NSCLC patients [9].…”