The role of lactic acid bacteria in production of bioactive peptides in fermented milk with antioxidant and antidiabetic properties

Shirkhan, Faezeh; Mirdamadi, Saeed; Mirzaei, Mahta; Akbari‐adergani, Behrouz; Nasoohi, Nikoo

doi:10.1007/s11694-023-01968-8

Cited by 4 publications

(2 citation statements)

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“…The assessment of the antidiabetic potential of bioactive peptides relied on the IER (inhibitory efficiency ratio), suggesting that the lowest molecular weight peptide portions exhibited the most pronounced hypoglycemic effects. Shirkhan et al (2023) underscored the noteworthy impact of fraction size on enzyme inhibition ( p < 0.05) with the >10 kDa peptide fraction. Lower molecular-weight peptide fractions showed increased proteolysis and antidiabetic properties and displayed the lowest inhibition rate for both enzymes.…”

Section: Resultsmentioning

confidence: 97%

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Peptidomics-based identification of antihypertensive and antidiabetic peptides from sheep milk fermented using Limosilactobacillus fermentum KGL4 MTCC 25515 with anti-inflammatory activity: in silico, in vitro, and molecular docking studies

Pipaliya,

Basaiawmoit,

Sakure

et al. 2024

Front. Chem.

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This study investigated the synthesis of bioactive peptides from sheep milk through fermentation with Limosilactobacillus fermentum KGL4 MTCC 25515 strain and assessed lipase inhibition, ACE inhibition, α-glucosidase inhibition, and α-amylase inhibition activities during the fermentation process. The study observed the highest activities, reaching 74.82%, 70.02%, 72.19%, and 67.08% (lipase inhibition, ACE inhibition, α-glucosidase inhibition, and α-amylase inhibition) after 48 h at 37°C, respectively. Growth optimization experiments revealed that a 2.5% inoculation rate after 48 h of fermentation time resulted in the highest proteolytic activity at 9.88 mg/mL. Additionally, fractions with less than 3 kDa of molecular weight exhibited superior ACE-inhibition and anti-diabetic activities compared to other fractions. Fermentation of sheep milk with KGL4 led to a significant reduction in the excessive production of NO, TNF-α, IL-6, and IL-1β produced in RAW 267.4 cells upon treatment with LPS. Peptides were purified utilizing SDS-PAGE and electrophoresis on 2D gels, identifying a maximum number of proteins bands ranging 10–70 kDa. Peptide sequences were cross-referenced with AHTPDB and BIOPEP databases, confirming potential antihypertensive and antidiabetic properties. Notably, the peptide (GPFPILV) exhibited the highest HPEPDOCK score against both α-amylase and ACE.

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Section: Resultsmentioning

confidence: 97%

“…Remarkably, their study substantiates our findings, indicating that the most potent ACE-I peptides are present in the molecular weight fraction below 3 kDa. Shirkhan et al (2023) examined the effects of LAB strains ( L. paracasei ssp. paracasei , L. delbrueckii , and L. helveticus ) on UHT milk.…”

Section: Resultsmentioning

confidence: 99%