The role of light-harvesting complex II in excess excitation energy dissipation: An in-vivo fluorescence study on the origin of high-energy quenching

Lokstein, Heiko; Härtel, Heiko; Hoffmann, P.; Woitke, Peter; Ренгер, Г.

doi:10.1016/1011-1344(94)07032-6

Cited by 47 publications

(44 citation statements)

References 33 publications

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“…Room temperature Chl fluorescence was measured as previously described (Lokstein et al, 1994) using a pulse amplitude modulation fluorometer (PAM 101-103; Walz, Effeltrich, Germany). The coefficient for qP was calculated as described by Bilger and Schreiber (1986).…”

Section: Chl Fluorescence Measurementsmentioning

confidence: 99%

“…Indeed, in mature leaves, qN, when calculated according to the Stern-Volmer formalism, has been correlated with Z accumulation upon light exposure (for reviews, see Demmig-Adams, 1990;Pfiindel and Bilger, 1994). The correlation appears to be less strict in plants possessing a reduced antenna complement (Lokstein et al, 1994;Jahns and Krause, 1994;Hartel and Lokstein, 1995). This raises the question about the mode of involvement of Z in the qN mechanism in these plants.…”

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confidence: 99%

“…There is mounting evidence that this proyortion of qN arises from the LHCs (for a recent review, see Horton et al, 1994). Studies of plants with altered antenna composition have served to substantiate this idea (Lokstein et al, 1993(Lokstein et al, , 1994 Briantais, * This work was supported in part by the Deutsche Forschungsgemeinschaft (grants Nos. Ho 1757/1 and Gr 936/4) and by a doctoral fellowship of the "Studienstiftung des deutschen Volkes"…”

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Kinetic Studies on the Xanthophyll Cycle in Barley Leaves (Influence of Antenna Size and Relations to Nonphotochemical Chlorophyll Fluorescence Quenching)

et al. 1996

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Xanthophyll-cycle kinetics as well as the relationship between the xanthophyll de-epoxidation state and Stern-Volmer type nonphotochemical chlorophyll (Chl) fluorescence quenching (qN) were investigated i n barley (Hordeom vulgare L.) leaves comprising a stepwise reduced antenna system. For this purpose plants of the wild type (WT) and the Chl b-less mutant chlorina 3613 were cultivated under either continuous (CL) or intermittent light (IML). Violaxanthin (V) availability varied from about 70% in the WT up to 97 to 98% in the mutant and IML-grown plants. I n CL-grown mutant leaves, de-epoxidation rates were strongly accelerated compared to the WT. This is ascribed to a different accessibility of V to the de-epoxidase due to the existence of two V pools: one bound to light-harvesting Chl a/b-binding complexes (LHC) and the other one not bound. Epoxidation rates ( k ) were decreased with reduction in LHC protein contents: kWT > k,,,,,,,, >> k,(IML This supports the idea that the epoxidase activity resides on certain LHC proteins. lrrespective of huge zeaxanthin and antheraxanthin accumulation, the capacity to develop qN was reduced stepwise with antenna sim. l h e qN leve1 obtained in dithiothreitol-treated CL-and IML-grown plants was almost identical with that in untreated IML-grown plants. l h e findings provide evidence that structural changes within the LHC proteins, mediated by xanthophyll-cycle operation, render the basis for the development of a maior proportion of qN.Higher plants are able to nonradiatively dissipate energy absorbed in excess to what can be utilized in photosynthesis and thus protect themselves from photodamage. This is reflected as qN. The buildup of qN was found to be influenced in a complex manner. The major (rapidly reversible) proportion of qN is associated with acidification of the thylakoid lumen in response to light exposure (for a review, see Horton and Ruban, 1992). There is mounting evidence that this proyortion of qN arises from the LHCs (for a recent review, see Horton et al., 1994). Studies of plants with altered antenna composition have served to substantiate this idea (Lokstein et al., 1993(Lokstein et al., , 1994 Briantais, * This work was supported in part by the Deutsche Forschungsgemeinschaft (grants Nos. Ho 1757/1 and Gr 936/4) and by a doctoral fellowship of the "Studienstiftung des deutschen Volkes"

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Section: Chl Fluorescence Measurementsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Kinetic Studies on the Xanthophyll Cycle in Barley Leaves (Influence of Antenna Size and Relations to Nonphotochemical Chlorophyll Fluorescence Quenching)

et al. 1996

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“…Natural systems have evolved light-harvesting complexes, comprising multiprotein ensembles embedded with pigment molecules to enhance photon absorption for photosynthesis (8). These pigment-rich sites are arranged such that excitation of a distal chromophore will ultimately result in energy transfer to a reaction center via a series of migration and transfer processes (9). Strategies to replicate natural light-harvesting complexes necessarily demand the organization of multiple chromophores (10), a requirement that makes self-assembly and supramolecular chemistry particularly well suited for such efforts (11)(12)(13).…”

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confidence: 99%

Synthesis and photophysical studies of self-assembled multicomponent supramolecular coordination prisms bearing porphyrin faces

Shi

Sánchez‐Molina

Cao

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Multicomponent self-assembly, wherein two unique donor precursors are combined with a single metal acceptor instead of the more common two-component assembly, can be achieved by selecting Lewis-basic sites and metal nodes that select for heteroligated coordination spheres. Platinum(II) ions show a thermodynamic preference for mixed pyridyl/carboxylate coordination environments and are thus suitable for such designs. The use of three or more unique building blocks increases the structural complexity of supramolecules. Herein, we describe the synthesis and characterization of rectangular prismatic supramolecular coordination complexes (SCCs) with two faces occupied by porphyrin molecules, motivated by the search for new multichromophore complexes with promising light-harvesting properties. These prisms are obtained from the self-assembly of a 90°Pt(II) acceptor with a meso-substituted tetrapyridylporphyrin (TPyP) and dicarboxylate ligands. The generality of this self-assembly reaction is demonstrated using five dicarboxylate ligands, two based on a rigid central phenyl ring and three alkyl-spaced variants, to form a total of five free-base and five Zn-metallated porphyrin prisms. All 10 SCCs are characterized by 31 P and 1 H multinuclear NMR spectroscopy and electrospray ionization mass spectrometry, confirming the structure of each self-assembly and the stoichiometry of formation. The photophysical properties of the resulting SCCs were investigated revealing that the absorption and emission properties of the free-base and metallated porphyrin prisms preserve the spectral features associated with free TPyP. supramolecular chemistry | metallacages T he use of sunlight is ubiquitous as the input for carbonneutral, renewable energy schemes (1). Every strategy that relies on solar energy conversion, ranging from direct conversion to electricity in photovoltaics (2) to the generation of fuels via electrocatalysis (3), photoanode (4, 5), or photocathode devices (6), or photocatalysis (7) requires that photons be absorbed by a molecule or material as the first step in providing the driving force for subsequent transformation. Natural systems have evolved light-harvesting complexes, comprising multiprotein ensembles embedded with pigment molecules to enhance photon absorption for photosynthesis (8). These pigment-rich sites are arranged such that excitation of a distal chromophore will ultimately result in energy transfer to a reaction center via a series of migration and transfer processes (9). Strategies to replicate natural light-harvesting complexes necessarily demand the organization of multiple chromophores (10), a requirement that makes self-assembly and supramolecular chemistry particularly well suited for such efforts (11-13). As such, a variety of approaches have been applied toward the development of new materials that exhibit broadband absorption and efficient energy transfer (14-16). The subsequent studies of such materials span investigations of the fundamental science behind energy migration and transfer, t...

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“…He also initiated fruitful collaboration and personal contacts among the authors of this obituary, which resulted in several joint publications (see e.g., Höxtermann et al 1982Höxtermann et al , 1986Lokstein et al 1993Lokstein et al , 1994Lokstein et al , 1995. Based on his communicative competence combined with high scientific reputation, the ''International Photosynthesis Workshops'', which were organized by him and his team in the 1970s and 1980s, became important platforms for international scientific exchange between researchers from Eastern and Western Europe and helped to surmount political boundaries.…”

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confidence: 99%

A tribute: Professor Dr. Paul Hoffmann (March 28, 1931–July 10, 2008), a scientist with a great collaborative spirit

Lokstein

Höxtermann²,

Leupold

et al. 2009

Photosynth Res

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The role of light-harvesting complex II in excess excitation energy dissipation: An in-vivo fluorescence study on the origin of high-energy quenching

Cited by 47 publications

References 33 publications

Kinetic Studies on the Xanthophyll Cycle in Barley Leaves (Influence of Antenna Size and Relations to Nonphotochemical Chlorophyll Fluorescence Quenching)

Kinetic Studies on the Xanthophyll Cycle in Barley Leaves (Influence of Antenna Size and Relations to Nonphotochemical Chlorophyll Fluorescence Quenching)

Synthesis and photophysical studies of self-assembled multicomponent supramolecular coordination prisms bearing porphyrin faces

A tribute: Professor Dr. Paul Hoffmann (March 28, 1931–July 10, 2008), a scientist with a great collaborative spirit

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