The Role of LncRNA MALAT-1 and MiRNA-9 in Psoriasis

Elamir, Azza; Shaker, Olfat; El-Komy, Mohamed HM.; Aboraia, Nesreen M.; sharabi, Mai

doi:10.21203/rs.3.rs-283734/v1

Cited by 4 publications

(6 citation statements)

References 18 publications

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“…Psoriasis is a skin disease that can be stimulated by many environmental factors, controlled by polygenes, such as MEG3 and MALAT1 [17, 18], and mediated by immune mechanisms. According to research findings, XIST plays an important role in a variety of diseases through regulating the inflammatory response [17, 19].…”

Section: Discussion/conclusionmentioning

confidence: 99%

LncRNA XIST Engages in Psoriasis via Sponging miR-338-5p to Regulate Keratinocyte Proliferation and Inflammation

Wang¹,

Jiang

Chen

et al. 2022

Skin Pharmacol Physiol

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Introduction: Psoriasis is an immune-mediated polygenic inflammatory skin disease, keratinocyte proliferation is an important mechanism. The study investigated the role and regulatory relationship between lncRNA XIST and miR-338-5p in psoriatic patients and cell models. Methods: Serum samples were collected from 55 psoriasis patients. HaCaT was recruited for the cell experiments, and induced by M5 cytokines to mimic psoriasis in vitro. XIST and miR-338-5p levels were detected via qRT-PCR. Cell viability under different treatments was evaluated using CCK-8. ELISA was applied to measure the concentration of inflammatory cytokines. The regulatory relationship was confirmed using luciferase reporter gene assay. Results: Serum XIST was elevated in patients with psoriasis, and can distinguish the psoriasis patients from healthy controls according to the ROC curve. High level of XIST was positively correlated with with PASI score and serum TNF-α, IL-17A and IL-22 concentrations in psoriasis patients. XIST silencing suppressed M5-induced keratinocyte proliferation and restrained the discharge of inflammatory cytokines (TNF-α, IL-17A, IL-22) and chemokines (CXCL1, CXCL8, CCL20). XIST can sponge miR-338-5p, and miR-338-5p downregulation abolished the inhibitory effect of XIST silencing on cell proliferation and inflammation. miR-338-5p was at high expression in the clinical serum samples from psoriasis patients. The target relationship between miR-338-5p and IL-6 was proved. Conclusion: LncRNA XIST is highly expressed in the serum of patients with psoriasis, and was positively correlated with disease severity and inflammation. XIST may regulate keratinocyte proliferation and inflammation via regulating miR-338-5p/IL-6 axis.

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Section: Discussion/conclusionmentioning

confidence: 99%

LncRNA XIST Engages in Psoriasis via Sponging miR-338-5p to Regulate Keratinocyte Proliferation and Inflammation

Wang¹,

Jiang

Chen

et al. 2022

Skin Pharmacol Physiol

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“…Psoriasis is chronic common autoimmune disease of the skin which affects most populations 2 . PD‐1 and ANG‐2 are two proteins that are reported to have a potential role in regulating the immune system's response to the cells of the human body as general 4,10 .…”

Section: Discussionmentioning

confidence: 99%

“…Psoriasis is considered as a chronic, recurrent disease that is related to the activation of T cells, including T helper 1 cells and 17 (Th1 and Th17). The T cells promote cutaneous inflammation and keratinocyte hyper proliferation 1,2 . As well the dysfunctional regulatory T (Treg) cells disrupt immune tolerance which in return leads to the induction of autoimmunity and auto‐inflammatory diseases like psoriasis 3 …”

Section: Introductionmentioning

confidence: 99%

“…The T cells promote cutaneous inflammation and keratinocyte hyper proliferation. 1,2 As well the dysfunctional regulatory T (Treg) cells disrupt immune tolerance which in return leads to the induction of autoimmunity and auto-inflammatory diseases like psoriasis. 3 PD-1, a glycoprotein expressed on the surface of various immune cells, including T cells consequently inhibits the function of effector T cells and promotes Treg cell activity.…”

Section: Introductionmentioning

confidence: 99%

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Are programmed cell death protein‐1 and Angiopoietins‐2 effective biomarkers for detection the severity of psoriatic patients?

Khatery

Shaker

El‐Tahlawi

et al. 2022

J of Cosmetic Dermatology

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Background Early detection of psoriasis is still an open discussion. Psoriatic lesions are characterized by red/scaly plaques affecting different body‐sites. Objectives To evaluate the levels of programmed cell death protein‐1(PD‐1) and Angiopoietins‐2(Ang‐2) in serum, lesional, and perilesional of psoriatic patients and correlate them with controls and disease severity. Subjects and Methods Serum samples were obtained from 40 participants subdivided equally into psoriatic and healthy controls, 4 mm punch_biopsy equally from lesional and perilesional skin of individuals. PD‐1/ANG‐2 ELISA kits were used for determining the serum and tissue levels among groups. Results Serum and tissue levels of PD‐1 and Ang‐2 were overexpressed in psoriatic patients compared with controls. There was a statistical difference between patients and controls in level of PD‐1(serum and tissue) with p‐value 0.006 and 0.0001, respectively. There was a statistical difference between both groups for ANG‐2(serum and tissue) with p‐value 0.03 and 0.0001, respectively. There were positive correlations between PASI score and PD‐1 in tissue (r = 0.467, p = 0.038). Also, positive correlation between the level of PD‐1 in serum and tissue (r = 0.369, p = 0.019), the serum levels of PD‐1 and ANG‐2 (r = 0.78, p > 0.0001), PD‐1 and Ang‐2 in tissue (r = 0.583,p = 0.0001) were detected. Conclusion PD‐1 and ANG‐2 can be highly recommended to determine the severity of psoriasis.

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“…Metastasis-associated lung adenocarcinoma transcript-1 (MALAT1) is a kind of LncRNA that acts as a crucial part in regulating the process of Lupus erythematosus (SLE) [10,11]. Some researchers have shown that MALAT1 is positively correlated to the area severity of psoriasis patients [12]. MALAT1 may promote the progression of psoriasis via miR-330-5p/S100A7 axis [13].…”

Section: Introductionmentioning

confidence: 99%

Interleukin-17 Regulates Keratinocyte Proliferation in Psoriasis Through the MALAT1/miR-125b/BRD4 Axis

Wang,

Chen,

Zhu

et al. 2024

j biomed nanotechnol

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This study investigated the role of LncRNA MALAT1 in psoriasis development. Serum from psoriasis patients and healthy subjects was collected, and IL-17 levels were measured. HaCaT cells were exposed to IL-17 and transfected with shMALAT1 or treated with Secukinumab. Cell viability, proliferation, and cell cycle were assessed, along with the expression of key cell cycle regulators. Various LncRNAs were analyzed, and the interaction between MALAT1 and p65 was confirmed through luciferase assays and ChIP assays. Results showed that IL-17 treatment increased cell proliferation and MALAT1 expression. p65 binding to the MALAT1 promoter enhanced keratinocyte proliferation. Secukinumab mitigated IL-17-induced cell proliferation and rescued miR-125b expression, which was reduced by IL-17. IL-17 also elevated BRD4 expression, which Secukinumab attenuated. In summary, IL-17 promotes HaCaT cell proliferation by upregulating MALAT1, with p65 facilitating this process. Secukinumab effectively counteracts these effects, restoring miR-125b levels and reducing BRD4 expression. These findings provide new insights into potential treatments for psoriasis.

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The Role of LncRNA MALAT-1 and MiRNA-9 in Psoriasis

Cited by 4 publications

References 18 publications

LncRNA XIST Engages in Psoriasis via Sponging miR-338-5p to Regulate Keratinocyte Proliferation and Inflammation

LncRNA XIST Engages in Psoriasis via Sponging miR-338-5p to Regulate Keratinocyte Proliferation and Inflammation

Are programmed cell death protein‐1 and Angiopoietins‐2 effective biomarkers for detection the severity of psoriatic patients?

Interleukin-17 Regulates Keratinocyte Proliferation in Psoriasis Through the MALAT1/miR-125b/BRD4 Axis

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