The role of lncRNA XIST/miR-211 axis in modulating the proliferation and apoptosis of osteoarthritis chondrocytes through CXCR4 and MAPK signaling

Li, Lei; Lv, Guohua; Wang, Bing; Kuang, Lei

doi:10.1016/j.bbrc.2018.07.015

Cited by 68 publications

(49 citation statements)

References 37 publications

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“…demonstrated that lncRNA MEG3 was downregulated and miR‐16 was upregulated in cartilage tissues of rat OA model, and this signaling pathway is involved in the initiation and development of OA. In contrast, another study demonstrated that lncRNA XIST can promote the proliferation of OA chondrocytes and promote apoptosis through the miR‐211/CXCR4 axis …”

Section: Introductionmentioning

confidence: 92%

“…In contrast, another study demonstrated that lncRNA XIST can promote the proliferation of OA chondrocytes and promote apoptosis through the miR-211/CXCR4 axis. 8 In the present study, we evaluated the possible miRNAs and lncRNAs that could regulate GPR120 by informatics assay, and further investigated these miRNAs/lncRNAs could regulate GPR120 and inflammation. We found that GPR120 is regulated by LINC00662 and miR-15b-5p, thereby contributing OA.…”

Section: Introductionmentioning

confidence: 99%

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Long noncoding RNA LINC00662‐miR‐15b‐5p mediated GPR120 dysregulation contributes to osteoarthritis

Lü¹,

Zhou

2020

Pathology International

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Previous studies demonstrated that dysregulation of G protein‐coupled receptor 120 (GPR120) plays a protective role in osteoarthritis (OA). However, the mechanism underlying how GPR120 is downregulated remains largely unknown. In the present study, we evaluated whether GPR120 is regulated by microRNAs (miRNAs) and long noncoding RNAs (lncRNAs). Our results show that GPR120 was negatively regulated by miR‐15b‐5p through targeting 3′ untranslated region (3′UTR), and that miR‐15b‐5p was negatively regulated by LINC00662. Further luciferase assay shows that LINC00662‐miR‐15b‐5p signaling pathway contributed the regulation of GPR120 expression. Functionally, the decreased of LINC00662 caused increased miR‐15b‐5p, thereby leading to decreased GPR120. The decreased GPR120 then contributes to increased expression of inflammatory factors including tumor necrosis factor α (TNF‐α), interleukin (IL)‐6 and IL‐8, cell apoptosis, and decreased apoptosis‐related protein levels including cleaved caspase‐3, cleaved caspase‐9, and Bax in cultured rat chondrocytes. In summary, the present study shows that LINC00662‐miR‐15b‐5p signaling pathway is involved in the regulation of GPR120, thereby contributing to arthritis.

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Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA LINC00662‐miR‐15b‐5p mediated GPR120 dysregulation contributes to osteoarthritis

Lü¹,

Zhou

2020

Pathology International

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“…miR‐211 (the sequence is same as miR‐204) was shown to hinder cell proliferation through the downregulation of SOX4 (Wang et al, ). Another group also reported that miR‐211 could modulate cell proliferation through CXCR4 and MAPK signalling (Li, Lv, Wang, & Kuang, ). miR‐204 was reported to inhibit cell proliferation and promote apoptosis through the downregulation of Bcl‐2 and Sirt1 (Ding & Lu, ), and was also associated with adipogenesis (He et al, ).…”

Section: Discussionmentioning

confidence: 97%

Small RNA sequencing of pectoral muscle tissue reveals microRNA‐mediated gene modulation in chicken muscle growth

Zhang

Qiu

et al. 2020

Animal Physiology Nutrition

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Sichuan mountainous black‐bone (SMB) chicken is a small‐sized black‐feathered chicken breed with low amount of meat, while Dahen (DH) chicken has a larger body size and a faster growth rate. MicroRNAs (miRNAs) are involved in various physiological processes, but their role in chicken muscle growth remains unclear. We aimed to investigate the miRNAs and pathways participating in the muscle growth of chicken. MiRNA profiles of four SMB chickens and four DH chickens were detected by small RNA sequencing. A total of 994 known miRNAs were identified, among which gga‐miR‐1a‐3p, gga‐miR‐148‐3p and gga‐miR‐133a‐3p exhibited the highest enrichment in both breeds of chickens. Thirty‐two miRNAs were differently expressed between SMB and DH chickens. The differently expressed miRNAs were mainly associated with fatty acid metabolism, immunity and MAPK activation‐related processes. Kyoto encyclopaedia of genes and genomes (KEGG) analysis showed that miRNAs were involved in the immunity‐related and MAPK signalling pathways. Moreover, miR‐204 was downregulated in DH chicken compared with SMB chicken, and significantly inhibited the expression of MAP3K13, which is involved in the MAPK pathway. It was confirmed through luciferase reporter assays that miR‐204 specifically inhibited the activity of MAP3K13. Our results helped demonstrate the potential molecular mechanisms of muscle growth in chickens and provide valuable information for chicken breeding.

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“…We studied the expression and the function of the cluster-specific DEGs of the new BM-MSCs subpopulations ( Supplementary Table 1: Sheet 3) with interesting results: 1) besides known markers or functional genes such as ALPL and collagen 1, some novel genes were also highly expressed in the osteoblast precursor cells. For instance, XIST, a long non-coding RNA (lncRNA) that regulates chondrocyte proliferation and apoptosis through MAPK signaling 30 , was highly expressed in the osteoblast precursor, suggesting that XIST might also be a novel regulator for osteogenesis. In addition, MCAM (CD146) was also differentially expressed in osteoblast precursor when compared with other cell subtypes.…”

Section: Cellular Taxonomy Of Bm-mscsmentioning

confidence: 99%

Single-cell RNA sequencing deconvolutes thein vivoheterogeneity of human bone marrow-derived mesenchymal stem cells

Wang

Li²,

Yang

et al. 2020

Preprint

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Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells, which have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat found in bone marrow. In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differentiate into various mesodermal lineages including osteoblast/osteocyte, chondrocyte, and adipocyte cells that are crucial for bone metabolism. While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. Biological functional annotations of transcriptomes suggest that osteoblast precursors may induce angiogenesis coupled with osteogenesis, and chondrocyte precursors may have the potential to differentiate into myocytes. We discovered transcripts for several cluster of differentiation (CD) markers that were highly expressed (e.g., CD167b, CD91, CD130 and CD118) or absent (e.g., CD74, CD217, CD148 and CD68) in BM-MSCs and could be novel markers for human BM-MSC purification. This study is the first systematic in vivo dissection of human BM-MSCs cell subtypes at the single-cell resolution, revealing insight into the extent of their cellular heterogeneity and bone homeostasis.

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The role of lncRNA XIST/miR-211 axis in modulating the proliferation and apoptosis of osteoarthritis chondrocytes through CXCR4 and MAPK signaling

Cited by 68 publications

References 37 publications

Long noncoding RNA LINC00662‐miR‐15b‐5p mediated GPR120 dysregulation contributes to osteoarthritis

Long noncoding RNA LINC00662‐miR‐15b‐5p mediated GPR120 dysregulation contributes to osteoarthritis

Small RNA sequencing of pectoral muscle tissue reveals microRNA‐mediated gene modulation in chicken muscle growth

Single-cell RNA sequencing deconvolutes thein vivoheterogeneity of human bone marrow-derived mesenchymal stem cells

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