The role of long non-coding RNAs in genome formatting and expression

Angrand, Pierre‐Olivier; Vennin, Constance; Bourhis, Xuefen Le; Adriaenssens, Éric

doi:10.3389/fgene.2015.00165

Cited by 118 publications

(100 citation statements)

References 117 publications

(109 reference statements)

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“…The mammalian genome encodes thousands of lncRNAs, many of which participate in a series of biological processes ranging from normal embryonic development to disease (Angrand et al, 2015;Philippen et al, 2015;Yarmishyn and Kurochkin, 2015). Understanding the exact mechanisms of regulation pattern of lncRNAs could help to shed light on the role of the same lncRNAs in other contexts, particularly in diseases.…”

Section: Discussionmentioning

confidence: 99%

The Role of Long Intergenic Noncoding RNA p21 in Vascular Endothelial Cells

Ding

et al. 2015

DNA and Cell Biology

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Large intergenic long noncoding RNA p21 (lincRNA-p21) has recently shown to play an important role in biological functions. However, the biological role of lincRNA-p21 in vascular endothelial cells remains unclear. In the present study, we investigated the role of lincRNA-p21 in vascular endothelial cells through gain- and loss-of-function studies and found that lincRNA-p21 promoted cell apoptosis and induced cell cycle progression. Furthermore, lincRNA-p21 acted as an endogenous sponge by directly binding to miR-130b and decreased miR-130b expression. In addition, miR-130b reversed the inhibitory effect of lincRNA-p21 on the growth of vascular endothelial cells. Taken together, our data highlight the pivotal role of lincRNA-p21 in the growth of vascular endothelial cells.

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Section: Discussionmentioning

confidence: 99%

The Role of Long Intergenic Noncoding RNA p21 in Vascular Endothelial Cells

Ding

et al. 2015

DNA and Cell Biology

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“…in the regulation of gene transcription, cell differentiation, genetic and epigenetic and other cellular activities (10,25,26). lncRNAs are considered to be new tumor biomarkers for cancer diagnosis and prognosis (8). The latest studies have demonstrated that the lncRNA CCAT2 promotes the MYC and Wnt signaling pathways in CRC in a positive feedback loop model (15).…”

Section: Discussionmentioning

confidence: 99%

“…With the development of whole genome sequencing technology, it has been revealed that <2% of the mammalian genome is in protein-encoded regions and the remainder of the genome contains non-coding RNAs (ncRNAs) (8). Those ncRNAs >200 nucleotides in length are termed long non-coding RNAs (lncRNAs) (9).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of long non‑coding RNA colon cancer‑associated transcript 2 is associated with advanced tumor progression and poor prognosis in patients with colorectal cancer

et al. 2017

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Abstract. The aim of the present study was to explore the clinicopathological and prognostic significance of long non-coding RNA (lncRNA) colon cancer-associated transcript 2 (CCAT2) expression in human colorectal cancer (CRC). Expression levels of lncRNA CCAT2 in CRC, adjacent non-tumor and healthy colon mucosa tissues were detected by quantitative polymerase chain reaction. The disease-free survival and overall survival rates were evaluated using the Kaplan-Meier method, and multivariate analysis was performed using Cox proportional hazard analysis. The expression level of lncRNA CCAT2 in CRC tissues was increased significantly compared with adjacent normal tissues or non-cancerous tissues. CCAT2 expression was observed to be progressively increased between tumor-node-metastasis (TNM) stages I and IV. A high level of CCAT2 expression was revealed to be associated with poor cell differentiation, deeper tumor infiltration, lymph node metastasis, distance metastasis, vascular invasion and advanced TNM stage. Compared with patients with low levels of CCAT2 expression, patients with high levels of CCAT2 expression had shorter disease-free survival and overall survival times. Multivariate analyses indicated that high CCAT2 expression was an independent poor prognostic factor. Therefore, increased lncRNA CCAT2 expression maybe a potential diagnostic biomarker for CRC, and an independent predictor of prognosis in patients with CRC.

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“…Long non-coding RNAs (lncRNAs) is a non-coding RNA, length of more than 200 nt, exist in eukaryotic cells, and which is highly conserved among different species of mammals [7, 8]. Recently, increasing evidences demonstrated that lncRNAs are key elements of pathologic and physiologic processes, and are vital regulators of inflammatory response [9, 10].…”

Section: Introductionmentioning

confidence: 99%

Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis

Sun

Han

et al. 2018

Cell Physiol Biochem

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Background/Aims: Several long non-coding RNAs (lncRNAs) play vital roles in osteoarthritis (OA), whereas the role of lncRNA RP11-445H22.4 in OA remains unclear. The study aimed to investigate the effect of lncRNA RP11-445H22.4 on lipopolysaccharide (LPS)-induced cell viability, apoptosis and inflammatory injury of OA. Methods: The expression of RP11-445H22.4, miR-301a and CXCR4 in human cartilage ATDC5 cells were altered by transfection, and then cells were exposed to 5 µg/ml LPS for 12 h. Then cell viability, apoptosis, apoptosis-related factors and inflammatory cytokines were analyzed by CCK-8, flow cytometry, western blot, RT-qPCR and ELISA, respectively. Dual-luciferase reporter assay was performed to assess the binging sites of RP11-445H22.4 and miR-301a. The signal pathways of NF-κB and MAPK/ ERK were determined by western blot. Results: LPS reduced cell viability, increased apoptosis and stimulated release of IL-1β, IL-6, IL-8 and TNF-α. However, RP11-445H22.4 inhibition significantly rescued LPS-induced injuries by promoting cell viability, suppressing apoptosis and inflammatory cytokines secretions in ATDC5 cells. In addition, miR-301a directly bound to RP11-445H22.4, and suppression of miR-301a inversed the effects of RP11-445H22.4 inhibition. Furthermore, CXCR4 was a direct target of miR-301a, and CXCR4 silencing increased cell viability, decreased apoptosis and inflammatory cytokines secretions in LPS-treated ATDC5 cells. Besides, we found that CXCR4 silencing blocked LPS-activated NF-κB and MAPK/ERK pathways. Conclusions: The study indicated that lncRNA RP11-445H22.4-miR-301a-CXCR4 axis played an important role in cartilage ATDC5 cells and provided a theoretical basis of lncRNA RP11-445H22.4 in OA.

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The role of long non-coding RNAs in genome formatting and expression

Cited by 118 publications

References 117 publications

The Role of Long Intergenic Noncoding RNA p21 in Vascular Endothelial Cells

The Role of Long Intergenic Noncoding RNA p21 in Vascular Endothelial Cells

Overexpression of long non‑coding RNA colon cancer‑associated transcript 2 is associated with advanced tumor progression and poor prognosis in patients with colorectal cancer

Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis

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