The role of macrophages in the removal of apoptotic B-cells in the sheep ileal Peyer's patch

Bhogal, Hardeep S.; Kennedy, Laurie J.; Babic, Kelly; Reynolds, John D.

doi:10.1016/j.dci.2003.12.006

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…Accordingly, B cell turnover in PP is a dynamic process characterized by high apoptotic rates to avoid excessive tissue expansion and to remove nonreactive B cells. Macrophages could be involved in this delicate process, as indicated by co-culturing experiments demonstrating phagocytosis of PP B cells by MHCII + macrophages (Bhogal et al, 2004). Consistently with these findings, we revealed that self-maintaining gMacs phagocytose apoptotic B cells within PP, as indicated by the engulfment of B cells and fragmented DNA and the expression of typical B cell markers within selfmaintaining gMacs.…”

Section: Galt-gmacs and Paneth Cell Niche-pasm Gmacssupporting

confidence: 87%

Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis

Schepper

Verheijden

Aguilera‐Lizarraga

et al. 2018

Cell

435

516

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Macrophages are highly heterogeneous tissue-resident immune cells that perform a variety of tissue-supportive functions. The current paradigm dictates that intestinal macrophages are continuously replaced by incoming monocytes that acquire a pro-inflammatory or tissue-protective signature. Here, we identify a self-maintaining population of macrophages that arise from both embryonic precursors and adult bone marrow-derived monocytes and persists throughout adulthood. Gene expression and imaging studies of self-maintaining macrophages revealed distinct transcriptional profiles that reflect their unique localization (i.e., closely positioned to blood vessels, submucosal and myenteric plexus, Paneth cells, and Peyer's patches). Depletion of self-maintaining macrophages resulted in morphological abnormalities in the submucosal vasculature and loss of enteric neurons, leading to vascular leakage, impaired secretion, and reduced intestinal motility. These results provide critical insights in intestinal macrophage heterogeneity and demonstrate the strategic role of self-maintaining macrophages in gut homeostasis and intestinal physiology.

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Section: Galt-gmacs and Paneth Cell Niche-pasm Gmacssupporting

confidence: 87%

Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis

Schepper

Verheijden

Aguilera‐Lizarraga

et al. 2018

Cell

435

516

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“…Mg-ATPase + dendritic cells are present at an early developmental stage [89] as are macrophages that stain with non-specific esterase and acid phosphatase [42,92]. The sheep ileal PP follicle also contains many tingible-body macrophages whose role it is to rapidly eliminate apoptotic cells [13].…”

Section: Follicle-associated Epithelium and Other Accessory Cells Of mentioning

confidence: 99%

“…Due to the extensive cell death occurring in the ileal PP the rapid clearance of these apoptotic cells is essential for tissue homeostasis. It is reported that macrophages resident in the ileal PP follicle mediate the phagocytosis and removal of discarded cells [13]. Using the DNA laddering assay, low molecular weight DNA is found in the BAQ44A -cells in the ileal PP but not detected in the BAQ44A + cells which are isolated from ileal PP [65].…”

Section: Apoptosis Of B-cell In Ileal and Jejunal Ppmentioning

confidence: 99%

The sheep and cattle Peyer’s patch as a site of B-cell development

et al. 2006

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-In sheep and cattle, the ileal Peyer's patch (PP), which extends one-two meters along the terminal small intestine, is a primary lymphoid organ of B-cell development. B-cell diversity in the ileal PP is thought to develop by combinatorial mechanisms, gene conversion and/or point mutation. These species also have jejunal PP that function more like secondary lymphoid tissues concerned with mucosal immune reactions. These two types of PP differ significantly in their histology, ontogeny and the extent of lymphocyte traffic. The prenatal development of follicles in the PP begins first in the jejunum during the middle of gestation and then in the ileum during late gestation. B-cells proliferate rapidly in the ileal PP follicle; up to five percent of these cells survive while the majority dies by apoptosis, perhaps driven by the influence of environmental antigen and/ or self-antigen. The surviving cells migrate from the ileal PP and populate the peripheral B-cell compartment. By adolescence, the ileal PP has involuted but the function of jejunal PP, compatible with a role as secondary lymphoid organ, continues throughout life. In this review, we focus on the development of PP as a site of B-cell repertoire generation, positive and negative B-cell selection, and the differences between ileal PP and jejunal PP.

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Macrophage heterogeneity in lymphoid tissues

Haan

Martı́nez-Pomares

2013

Semin Immunopathol

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Macrophages in lymphoid organs exhibit a wide variety of phenotypes and functions. These cells excel in the removal of apoptotic cells that arise during the generation of immune cells and are thereby essential for the prevention of auto-immune responses. In addition to this macrophages in the secondary lymphoid organs form an important barrier for spreading of infections by phagocytosis of pathogens and the activation of both innate and adaptive immune responses. Thus, the remarkable ability of macrophages to phagocytose and handle a wide range of self and non-self material and to produce immunomediators is effectively exploited within lymphoid organs to regulate immune activation.

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The role of macrophages in the removal of apoptotic B-cells in the sheep ileal Peyer's patch

Cited by 5 publications

References 31 publications

Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis

Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis

The sheep and cattle Peyer’s patch as a site of B-cell development

Macrophage heterogeneity in lymphoid tissues

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