The Role of microRNAs in Bovine Infection and Immunity

Lawless, Nathan; Végh, Péter; O’Farrelly, Cliona; Lynn, David J.

doi:10.3389/fimmu.2014.00611

Cited by 55 publications

(49 citation statements)

References 70 publications

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“…Although limited research has been conducted, microRNAs have been implicated in the immune response associated with BRD infection in cattle (Lawless et al . ). For example, four microRNAs ( bta‐miR‐24‐3p, bta‐miR‐92a, bta‐423‐5p and bta‐let‐7b ) were differentially expressed in beef cattle that were seropositive for M .…”

Section: Resultsmentioning

confidence: 97%

Gene set enrichment analysis of SNP data in dairy and beef cattle with bovine respiratory disease

2018

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Bovine respiratory disease (BRD) is a complex disease that is associated with infection by bacterial and viral pathogens when cattle fail to adequately respond to stress. The objective of this study was to use gene set enrichment analysis of SNP data (GSEA-SNP) and a network analysis (ingenuity pathway analysis) to identify gene sets, genes within gene sets (leading-edge genes) and upstream regulators associated with BRD in pre-weaned dairy calves and beef feedlot cattle. BRD cases and controls were diagnosed using the McGuirk health scoring system. Holstein calves were sampled from commercial calf-raising facilities in California (1003 cases and 1011 controls) and New Mexico (376 cases and 372 controls). Commercial feedlot cattle were sampled from Colorado (500 cases and 499 controls) and Washington (504 cases and 497 controls). There were 102 and 237 unique leading-edge genes identified in the dairy calf and beef cattle populations respectively. Six leading-edge genes (ADIPOQ, HTR2A, MIF, PDE6G, PRDX3 and SNCA) were associated with BRD in both dairy and beef cattle. Network analysis identified glucose as the most influential upstream regulator in dairy cattle, whereas in beef cattle, TNF was the most influential upstream regulator. The genes, gene sets and upstream regulators associated with BRD have common functions associated with immunity, inflammation and pulmonary disease and provide insights into the mechanisms that are critical to BRD susceptibility in cattle.

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Section: Resultsmentioning

confidence: 97%

Gene set enrichment analysis of SNP data in dairy and beef cattle with bovine respiratory disease

2018

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“…More identifications of the bovine miRNA expression atlas in different tissues and cells are necessary to further fill in the gap (Lawless et al . ,). These newly identified miRNAs could significantly expand the bovine miRNA spectrum and refine their annotations.…”

Section: Resultsmentioning

confidence: 99%

“…Using next‐generation sequencing and RT‐PCR approaches, several studies have identified differentially expressed bovine miRNAs, target genes, KEGG pathways and related regulatory networks and pointed out their roles in regulating host response to S. aureus or Escherichia coli , in vivo and in vitro , at early stages of infection (Lawless et al . , ,; den Breems et al . ; Jin et al .…”

Section: Introductionmentioning

confidence: 99%

Solexa sequencing and custom microRNA chip reveal repertoire of microRNAs in mammary gland of bovine suffering from natural infectious mastitis

Jiang

Liu

et al. 2018

Animal Genetics

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Identification of microRNAs (miRNAs), target genes and regulatory networks associated with innate immune and inflammatory responses and tissue damage is essential to elucidate the molecular and genetic mechanisms for resistance to mastitis. In this study, a combination of Solexa sequencing and custom miRNA chip approaches was used to profile the expression of miRNAs in bovine mammary gland at the late stage of natural infection with Staphylococcus aureus, a widespread mastitis pathogen. We found 383 loci corresponding to 277 known and 49 putative novel miRNAs, two potential mitrons and 266 differentially expressed miRNAs in the healthy and mastitic cows' mammary glands. Several interaction networks and regulators involved in mastitis susceptibility, such as ALCAM, COL1A1, APOP4, ITIH4, CRP and fibrinogen alpha (FGA), were highlighted. Significant down-regulation and location of bta-miR-26a, which targets FGA in the mastitic mammary glands, were validated using quantitative real-time PCR, in situ hybridization and dual-luciferase reporter assays. We propose that the observed miRNA variations in mammary glands of mastitic cows are related to the maintenance of immune and defense responses, cell proliferation and apoptosis, and tissue injury and healing during the late stage of infection. Furthermore, the effect of bta-miR-26a in mastitis, mediated at least in part by enhancing FGA expression, involves host defense, inflammation and tissue damage.

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“…MiRNAs have been shown to play roles in bovine infection and immunity in a wide range of tissues [54,[106][107][108][109][110][111][112][113]. For mammary gland, Naeem et al [114] studied the expression of 14 miRNAs (miR-10a, miR-15b, miR-16a, miR-17, miR-21, miR-31, miR-145, miR-146a, miR146b, miR-155, miR-181a, miR-205, miR-221 and miR-223) in MG tissue challenged with Streptococcus uberis and identified three downregulated miRNAs (miR-181a, miR-16 and miR-31) and one upregulated miRNA (miR-223) in infected versus healthy tissue.…”

Section: Microrna Functions In Mammary Gland Healthmentioning

confidence: 99%

Non-Coding RNA Roles in Ruminant Mammary Gland Development and Lactation

Ngoc¹,

Ibeagha‐Awemu²

2017

Current Topics in Lactation

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The ruminant mammary gland (MG) is an important organ charged with the production of milk for young and human nourishment. ) are a class of untranslated RNA molecules that function to regulate gene expression, associated biochemical pathways and cellular functions and are involved in many biological processes. This chapter presents a review of the current state of knowledge on the role of ncRNAs (particularly miRNAs and lncRNAs) in the MG and lactation processes, lactation signalling pathways, lipid metabolism, MG health of ruminants as well as miRNA roles in milk recipients. Finally, the potential application of new genome editing technology for ncRNA studies in MG development, the lactation process and milk components is presented.

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The Role of microRNAs in Bovine Infection and Immunity

Cited by 55 publications

References 70 publications

Gene set enrichment analysis of SNP data in dairy and beef cattle with bovine respiratory disease

Gene set enrichment analysis of SNP data in dairy and beef cattle with bovine respiratory disease

Solexa sequencing and custom microRNA chip reveal repertoire of microRNAs in mammary gland of bovine suffering from natural infectious mastitis

Non-Coding RNA Roles in Ruminant Mammary Gland Development and Lactation

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