The Role of Mitochondria in Syncytiotrophoblast Cells: Bioenergetics and Steroidogenesis

Martı́nez, Federico; Milán, Rebeca; Flores-Herrera, Óscar; Olvera-Sánchez, Sofía; Gómez-Chang, Erika; Espinosa-García, María Teresa

doi:10.5772/32938

Cited by 2 publications

(1 citation statement)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By 22 weeks of gestation, the mean placental weight is one third of that of the fetus while the mid-gestation fetus consumes only 25% of the oxygen required by the placenta [ 29 ]. The placenta has also been shown, like for the brain, to mainly use glucose to support its high pace metabolism [ 30 , 31 ]. The placenta acts like the effector of the intrauterine environment to drive embryonic development [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Gene Expression Profiles Are Associated with Maternal Psychosocial Stress in Pregnancy and Infant Temperament

2015

View full text Add to dashboard Cite

BackgroundGene-environment interactions mediate through the placenta and shape the fetal brain development. Between the environmental determinants of the fetal brain, maternal psychosocial stress in pregnancy has been shown to negatively influence the infant temperament development. This in turn may have adverse consequences on the infant neurodevelopment extending throughout the entire life-span. However little is known about the underlying biological mechanisms of the effects of maternal psychosocial stress in pregnancy on infant temperament. Environmental stressors such as maternal psychosocial stress in pregnancy activate the stress response cascade that in turn drives the increase in the cellular energy demand of vital organs with high metabolic rates such as, in pregnancy, the placenta. Key players of the stress response cascade are the mitochondria.ResultsHere, we tested the expression of all 13 protein-coding genes encoded by the mitochondria in 108 placenta samples from the Stress in Pregnancy birth cohort, a study that aims at determining the influence of in utero exposure to maternal psychosocial stress in pregnancy on infant temperament. We showed that the expression of the protein-coding mitochondrial-encoded gene MT-ND2 was positively associated with indices of maternal psychosocial stress in pregnancy including Prenatal Perceived Stress (β = 0.259; p-regression = 0.004; r2-regression = 0.120), State Anxiety (β = 0.218; p-regression = 0.003; r2-regression = 0.153), Trait Anxiety (β = 0.262; p-regression = 0.003; r2-regression = 0.129) and Pregnancy Anxiety Total (β = 0.208; p-regression = 0.010; r2-regression = 0.103). In the meantime MT-ND2 was negatively associated with the infant temperament indices of Activity Level (β = -0.257; p-regression = 0.008; r2-regression = 0.165) and Smile and Laughter (β = -0.286; p-regression = 0.036; r2-regression = 0.082). Additionally, MT-ND6 was associated with the maternal psychosocial stress in pregnancy index of Prenatal Perceived Stress (β = -0.231; p-regression = 0.004; r2-regression = 0.120), while MT-CO2 was associated with the maternal psychosocial stress in pregnancy indices of State Anxiety (β = 0.206; p-regression = 0.003; r2-regression = 0.153) and Trait Anxiety (β = 0.205; p-regression = 0.003; r2-regression = 0.129).ConclusionsOur data support the role of mitochondria in responding to maternal psychosocial stress in pregnancy, as assessed in placenta, while also suggesting an important role for the mitochondria in the infant temperament development.

show abstract

Section: Introductionmentioning

confidence: 99%

Mitochondrial Gene Expression Profiles Are Associated with Maternal Psychosocial Stress in Pregnancy and Infant Temperament

2015

View full text Add to dashboard Cite

show abstract

Use of Glucose, Glutamine, and Fatty Acids for Trophoblast Respiration in Lean Women, Women With Obesity, and Women With Gestational Diabetes

Wang

Bucher

Myatt

2019

The Journal of Clinical Endocrinology &Amp; Metabolism

View full text Add to dashboard Cite

Objective Maternal obesity and gestational diabetes mellitus (GDM) are associated with adverse outcomes, particularly with a male fetus. The composition and amount of substrate supplied to the placenta are altered in these conditions. We hypothesized that there are sexually dimorphic differences in utilization of glucose, fatty acids, and glutamine between trophoblast of lean women, women with obesity, and women with GDM. Design Trophoblasts were isolated from term male or female placentas from lean women, women with obesity, or women with GDM (n = 4 to 6 per group), and syncytiotrophoblast formed during 72 hours before measuring mitochondrial respiration by a fuel flex assay (Seahorse XF96 analyzer). Dependency, capacity, and flexibility for use of glucose, glutamine, and fatty acids were measured with western blot of glucose transporter GLUT1, glutaminase, and carnitine palmitoyltransferase 1A. Results Sexual dimorphism in syncytiotrophoblast fuel utilization was seen in women with GDM vs lean women with a significant increase in glucose dependency in males and glucose capacity in females, whereas for glutamine, capacity was significantly decreased in males and females but dependency significantly decreased only in females. Fatty acid dependency and capacity significantly increased in male trophoblast and capacity in female trophoblast of women with GDM vs either lean women or women with obesity. In male but not female trophoblast, flexibility to use all three fuels significantly decreased from lean women to women with obesity and women with GDM. In male trophoblast there were significant associations between GLUT1 and glucose dependency (positive) and flexibility (negative). Conclusions Human syncytiotrophoblast utilizes glutamine for mitochondrial respiration. Utilization of glucose, fatty acids, and glutamine changes in a sexually dimorphic manner with obesity and GDM, predominantly with a male placenta.

show abstract

The Role of Mitochondria in Syncytiotrophoblast Cells: Bioenergetics and Steroidogenesis

Cited by 2 publications

References 135 publications

Mitochondrial Gene Expression Profiles Are Associated with Maternal Psychosocial Stress in Pregnancy and Infant Temperament

Mitochondrial Gene Expression Profiles Are Associated with Maternal Psychosocial Stress in Pregnancy and Infant Temperament

Use of Glucose, Glutamine, and Fatty Acids for Trophoblast Respiration in Lean Women, Women With Obesity, and Women With Gestational Diabetes

Contact Info

Product

Resources

About